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A Lactobacillus plantarum that improves sensitive facial skin and repairs the skin barrier

A technology of Lactobacillus plantarum and viable count, applied in the field of microorganisms, can solve problems such as side effects, complex drug action mechanism, dependence, etc., and achieve the effects of improving survival rate, improving barrier damage, and promoting cell migration.

Active Publication Date: 2022-08-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on drugs for the treatment of various skin diseases, the complex mechanism of action of drugs will bring dependence and side effects

Method used

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  • A Lactobacillus plantarum that improves sensitive facial skin and repairs the skin barrier
  • A Lactobacillus plantarum that improves sensitive facial skin and repairs the skin barrier
  • A Lactobacillus plantarum that improves sensitive facial skin and repairs the skin barrier

Examples

Experimental program
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Effect test

preparation example Construction

[0060] 2. Preparation of Topical Probiotic Samples

[0061] (1) Probiotic fermentation supernatant: take the cultured probiotics by centrifugation at 8000r for 15 min to take the supernatant; adjust the pH to 7.2-7.4; filter and sterilize with a 0.22 μm disposable filter, and store in a -20°C refrigerator after packaging.

[0062] (2) Probiotic intracellular substance: take 10 mL of cultured probiotic bacteria to centrifuge to collect bacterial mud, add 1 mL of PBS solution to dissolve; add liquid nitrogen to grind and crush, collect bacterial mud suspension; add 1 mL of PBS solution to dissolve, ultrasonically crushed; centrifuge at 8000 r The supernatant was collected for 10 min; sterilized by filtration with a 0.22 μm disposable filter, and stored in a refrigerator at -20° C. after packaging (0.1 g of wet bacteria mud obtained 1 mL of intracellular supernatant).

[0063] Complete medium: 5% fetal bovine serum (FBS), 100 U / mL penicillin, 100 mg / mL streptomycin, 95% DMEM medi...

Embodiment 1

[0064] Example 1: Screening and identification of Lactobacillus plantarum

[0065] Specific steps are as follows:

[0066] (1) Isolation and screening of Lactobacillus plantarum

[0067] (1) collecting kimchi samples from different regions, and enriching the samples in MRS medium containing sorbitol for 12h;

[0068] (2) The enriched sample in step (1) is subjected to gradient dilution and then coated on the MRS solid plate added with 0.02% olfactory cresyl violet, and cultured for 24-48 hours;

[0069] (3) select the single colony with obvious discoloration circle on the MRS plate in step (2) and conform to the basic form of lactic acid bacteria to carry out streak purification on the MRS solid plate, and screen and isolate the lactic acid bacteria;

[0070] (4) The single colony on the plate in step (3) is picked and cultured in the liquid MRS medium for 24 hours, and then Gram-stained, and Gram-positive bacteria are selected for the subsequent test.

[0071] (2) Prelimin...

Embodiment 2

[0092] Example 2: Effect of Lactobacillus plantarum on SDS-induced HaCaT cell survival

[0093] Specific steps are as follows:

[0094] After rewarming the cryopreserved HaCaT cells at 37°C, centrifuge at 1000 rpm for 5 min to take the pellet; after washing the pellet once with complete medium, resuspend the cells in complete medium and count to obtain a cell resuspend; Inoculated into a 10cm petri dish at 37°C, gas phase containing 5% (v / v) CO 2 The cells were cultured in a cell incubator, and the complete medium was replaced the next day and continued at 37°C with 5% (v / v) CO in the gas phase. 2 cultured in a cell incubator. Cells were passaged when they had grown to 70% to 80% density of the dish.

[0095] HaCaT cells with good growth status were selected, the HaCaT cells were digested with trypsin at a concentration of 2.5 g / L, centrifuged, resuspended in complete medium, and counted to obtain a cell resuspension; the cell resuspension was 5× 10 3 Inoculate each well ...

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Abstract

The invention discloses a Lactobacillus plantarum capable of improving sensitive facial skin and repairing skin barrier, belonging to the technical field of microorganisms and the technical field of medicine. The present invention provides a strain of Lactobacillus plantarum CCFM1158. The Lactobacillus plantarum CCFM1158 can alleviate human cell barrier damage, and is embodied in: (1) significantly improving the survival rate of SDS-induced HaCaT cells; (2) significantly improving SDS Barrier damage caused by induction of HaCaT cells; (3) the ability to significantly promote cell migration; (4) can effectively promote the expression of natural moisturizing factor and barrier integrity factor FLG, therefore, Lactobacillus plantarum CCFM1158 in the preparation of prevention or treatment of skin barrier damage The products have huge application prospects.

Description

technical field [0001] The invention relates to a Lactobacillus plantarum capable of improving sensitive facial skin and repairing skin barrier, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] The human skin has various functions such as excretion, sensation, secretion, metabolism, barrier, absorption, immunity, thermoregulation, etc. The barrier is its most basic function. Once the skin barrier function is damaged, the risk of various skin diseases will increase significantly. Doing a good job in skin barrier function repair and treatment is an important measure to reduce the occurrence of skin diseases. Studies have found that abnormalities in the structure and function of the skin barrier are related to the pathological characteristics of different skin diseases. Doing a good job in skin barrier repair is an important method to prevent bacterial invasion, and also helps to reduce skin inflammation and r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K8/99A61Q19/00A61K35/747A61P17/18C12R1/25
CPCA61K8/99A61Q19/00A61K35/747A61P17/18A61K2800/10A61Q19/10A61Q19/08
Inventor 崔树茂毛丙永孙媭唐鑫杨波陆文伟赵建新
Owner JIANGNAN UNIV
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