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Composition for improving skin barrier damage and/or alleviating skin inflammation, containing 3,5-dicaffeoylquinic acid as active ingredient

A technique for caffeoylquinic acid and skin inflammation, which is applied in the field of compositions for improving skin barrier damage and/or skin inflammation alleviation, can solve the problem that the effect of alleviating skin inflammation is not yet understood, and can improve skin barrier damage and alleviate skin inflammation. Effects of skin inflammation

Pending Publication Date: 2021-06-18
KOREA UNIV RES & BUSINESS FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, 3,5-dicaffeoylquinic acid (3,5-dicaffeoylquinic acid) isolated from the extract of flax (Aster glehni) has not been known to improve skin barrier damage or relieve skin inflammation

Method used

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  • Composition for improving skin barrier damage and/or alleviating skin inflammation, containing 3,5-dicaffeoylquinic acid as active ingredient
  • Composition for improving skin barrier damage and/or alleviating skin inflammation, containing 3,5-dicaffeoylquinic acid as active ingredient
  • Composition for improving skin barrier damage and/or alleviating skin inflammation, containing 3,5-dicaffeoylquinic acid as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1: preparation of flax extract

[0095] Purchased flax vegetables (Asterglehni: AG) that were peeled off and dried in Ulleungdo (N37°30', E130°52') in November 2012, and received the honorary title of Prof. Luk Chang-soo (Department of Pharmacology, Kyung Hee University, Seoul) Confirmation, the voucher specimen (971-12A-P) is kept in the plant specimen box of the Korea Institute of Science and Technology.

[0096] To obtain a methanol-soluble extract, minced 12 kg AG was extracted 3 times with 70 L of methanol at room temperature. 2.6 kg of the dry extraction residue were suspended in water and partitioned successively with ethyl acetate. The ethyl acetate fraction was evaporated under reduced pressure to obtain 41.0 g of a residue. The ethyl acetate fraction of AG was analyzed by reversed-phase high-speed liquid chromatography (Waters 1500 series systems) using a 2996 PDA detector (254 nm, Waters, Worcester, MA, USA).

[0097] For separation, 10 μL of sa...

Embodiment 2

[0100] Example 2: Culture of Skin Keratinocytes (HaCaT)

[0101] HaCaT cells (human keratinocytes) were incubated at 37°C in 5% CO 2 In an incubator, culture was carried out in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% antibiotic-antibacterial solution. Replace the cell culture medium with fresh DMEM medium every 48-72 hours. HaCaT cells from passage 5 to 17 were placed in 8-well chamber slides at 1x 10 per well 4 Density of cells, or in 6-well culture plates in DMEM containing 10% fetal bovine serum and 1% antibiotic-antimicrobial, at 1x 10 per well 6 Cell density, spread in well plate. Cells were incubated at 37°C, 5% CO 2 After culturing in the incubator for 24 hours to 48 hours, the medium was replaced with DMEM containing 1% fetal bovine serum. Cells were then treated for 24 hours with DNCB 5 μM (Sigma-Aldrich, Louis, MO) or SDS 30 μM (Sigma-Aldrich) containing AG extract (50 μg) and the PPARδ antagonist GSK0660 (Sigma-A...

Embodiment 3

[0102] Example 3: When treated with flaxseed extract, observe the protein expression of PPARδ, AMPK, SPTLC2 and TRPV4 in HaCaT cells treated with DNCB or SDS

[0103] In order to evaluate the protein expression levels of PPARδ, AMPK and SPTLC2 in HaCaT cells treated with AG and SDS or DNCB, Western blot was performed after treatment according to the treatment conditions in Example 2.

[0104] The protein concentration of the samples was evaluated according to the Coomassie brilliant blue method (Bradford method). 10 μg of the extracted protein was loaded on a 10% sodium dodecyl sulfate (SDS) polyacrylamide gel, and Western blotting was performed on a nitrocellulose membrane for 90 minutes. The membrane was blocked overnight with 5% skim milk and washed 3 times with TBS-T during 10 minutes. The primary antibody was allowed to bind to the membrane for 2 hours at room temperature. The primary antibody to PPARδ was purchased from Abcam. Primary antibodies to whole and phosphory...

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Abstract

The present invention relates to a composition for improving skin barrier damage and / or alleviating skin inflammation, and enables the improvement of skin barrier damage and the alleviation of skin inflammation through the application of Aster glehni extract-isolated 3,5-dicaffeoylquinic acid as an active ingredient of a cosmetic composition and a pharmaceutical composition, thereby having effects of preventing and improving atopic dermatitis.

Description

technical field [0001] The present invention relates to a composition for improving skin barrier damage and / or alleviating skin inflammation, and more specifically, to a composition for skin containing 3,5-dicaffeoylquinic acid (3,5-dicaffeoylquinic acid) Composition for improving barrier damage and / or alleviating skin inflammation. Background technique [0002] The skin, also known as the integument, is made up of three main layers: the epidermis, the dermis, and the subcutaneous tissue (hypodermis). In particular, the epidermis is the outer area of ​​the skin, and its structure is composed of 4 layers, which can be divided into the basal layer, spinous layer, granular layer, and stratum corneum (the outermost thin layer of the skin). Keratinocytes are the main cells that make up the epidermis and contribute to the body's defensive barrier through keratinization. In the spinous layer, keratinocytes produce keratin 1 and 10 (keratin 1 and 10), which are major components of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61K36/28A61K9/00A61P17/00A61P29/00A61K8/368A61K8/9789A61Q19/00A23L33/10
CPCA61P17/00A61P29/00A61K8/9789A61Q19/00A61K9/00A61K36/28A61K31/192A23L33/10A61K8/368A61K31/216A23L33/105A23L2/52A23L2/02A61K9/0014A23V2002/00A23V2250/21A23V2200/318A61K2236/333A61K2236/39
Inventor 徐汞锡李庸稙金亨子秦昶培
Owner KOREA UNIV RES & BUSINESS FOUND
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