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Behcet disease marker microorganisms and uses thereof

A technology for microorganisms and Behcet's disease, which is applied in the direction of microorganisms, microorganism-based methods, and detection/testing of microorganisms, and can solve problems such as activation and decreased response of regulatory T cells

Pending Publication Date: 2022-04-12
上海锐翌医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This study is the first to demonstrate that butyrate production is significantly reduced in patients with Behcet's disease. However, butyrate can promote the differentiation of regulatory T cells, so the obtained results suggest that defects in butyrate production may lead to regulatory T cell differentiation. Decreased response and activation of immunopathological T effector responses

Method used

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  • Behcet disease marker microorganisms and uses thereof
  • Behcet disease marker microorganisms and uses thereof
  • Behcet disease marker microorganisms and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Identification of biomarkers

[0073] In this example, the inventors studied the fecal samples of 16 Behcet's disease patients and 36 healthy controls to obtain the characteristics of the microbial community and functional components of the intestinal flora. In general, the inventors obtained 334Gb high-quality sequencing data through experimental sequencing. Metagenomic analysis showed that 11 microbial species were closely related to Behcet's disease, 4 of which were enriched in the gut microbes of healthy people, and 7 were enriched in the gut microbes of Behcet's patients.

[0074] 1. Sample collection and DNA extraction

[0075] All the samples were from the First Affiliated Hospital of Chongqing Medical University. A total of 16 Chinese Behcet’s patients’ stool samples and 36 healthy individuals’ stool samples were collected in the experiment. The fresh stool samples of each individual were divided into 200mg / parts, a total of 5 immediately stored in a...

Embodiment 2

[0109] Verification of Example 2 Microbial Species Marker

[0110] In order to verify the findings in Example 1, the inventors further analyzed the abundance of the 17 bacterial genera in the stool samples of 18 healthy people and 8 Behcet's patients in the verification group, and according to the presence of each species in the healthy group The 17 microbial species markers were deleted, and the DNA extraction, sequencing and species abundance analysis of the verified population were performed with reference to Example 1.

[0111] The verification results are as follows: Among the 6 species enriched in the healthy population, 4 of them were verified with high quality in the verification set (p_values<0.05), the mean and P value results of the verification of the microbial species markers enriched in healthy people As shown in Table 2, they are Candidatus_Methanomethylophilus_alvus, Clostridium_sp_CAG349, Coprococcus_sp_CAG782, and Methanoculleus_sp_CAG1088, respectively. Met...

Embodiment 3

[0120] The detection of embodiment 3 individual state

[0121] In this example, the inventors used 36 stool samples to detect the individual status of the samples.

[0122] Determine Atkinsonella_texensis, Paspalum ergot bacteria (Claviceps_paspali), Corynebacterium argentoratense (Corynebacterium_argentoratense), Epichloe_aotearoae (Epichloe_aotearoae), Shell coccidium (Macrophomina_phaseolina), maltophilia shown in Table 3 in each stool sample with reference to the method of embodiment 2 The abundance of Stenotrophomonas_maltophilia and Stenotrophomonas_rhizophila, to determine whether the abundance of these 7 strains in each sample falls within the respective abundance of the disease control group or the healthy control group With a 95% confidence interval, it is determined that the abundance of these 7 bacterial species falls into the corresponding interval of the disease group. The individual corresponding to the sample is a Behçet disease patient, and it is determined th...

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Abstract

The invention provides a behcet disease marker microorganism and application thereof, and the behcet disease marker microorganism comprises a first microorganism set, so that the invention further provides a kit which comprises a reagent suitable for detecting at least one strain in the first microorganism set, the first microorganism set is prepared from the following strains: Atkinsonlatexis, Claviceppaspali, Corynebacterium glutamicum, Epichia pastoris (Epichia pastoris), Macrophaphaeolina, Stenotrophomonas maltophilia and Stenotrophomonas rhizophila, and the first microorganism set is prepared from the following strains: Atkinsonlatexis, Claviceppaspali, Corynebacterium glutamicum, Epichia pastoris (Epichia pastoris), Stenotrophomonas maltophilia, Stenotrophomonas rhizophila, Stenotrophomonas sp., Stenotrophomonas sp., Stenotrophomonas sp., Stenotrophomonas sp. And Stenotrophomonas sp. The marking microorganism provided by the invention has significant difference in abundance between healthy people and behcet disease patients, and can be used as a marker for detecting and / or treating behcet disease.

Description

technical field [0001] The present invention relates to the field of biotechnology, specifically, the present invention relates to Behcet's disease marker microorganisms and their uses, more specifically, the present application relates to a kit, the use of the reagent in the preparation of the kit, and the method for preventing or treating Behcet's disease A pharmaceutical composition or a food composition for the disease, a method for determining whether an individual suffers from Behcet's disease, a device for determining whether an individual has Behçet's disease, a device, and a method for screening drugs. Background technique [0002] Behcet's disease (BD) is an autoinflammatory disease involving multiple organs, with recurrent oral and genital ulcers, skin lesions, and uveitis as typical clinical manifestations. BD mostly occurs in young and middle-aged people, with repeated inflammation and difficult treatment, and the resulting blindness is mostly irreversible blind...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6895G16B30/00G16B30/20G16B40/00G16B50/30A23L33/135A61K35/74A61P29/00A61P1/02A61P17/00A61P17/02C12R1/645C12R1/15C12R1/01
Inventor 吴春燕谢秋瑾沈秀萍
Owner 上海锐翌医学检验实验室有限公司
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