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Preparation and application method of radioactive decontamination strippable film biodegradable microbial agent

A biodegradation and radioactive technology, applied in microorganism-based methods, biochemical equipment and methods, radioactive purification, etc., can solve problems such as the limitations of photocatalytic degradation technology

Pending Publication Date: 2022-04-15
SOUTHWEAT UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in places where sunlight cannot be directly irradiated, such as a large number of synthetic polymer wastes such as landfills, composting sites, and plastic film buried parts, the photocatalytic degradation technology is greatly limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Bacillus licheniformis LS04 ( Bacillus licheniformis ), Bacillus pumilus LCOl ( Bacillus pumilus ), Bacillus amyloliquefaciens LC02 ( Bacillus amyloliquefaciens ), Bacillus subtilis LS02 ( Bacillus subtilis ) were inoculated in glucose 10.0g / L, peptone 10.0g / L, CaCl 2 1.5g / L, MgSO 4 1.5g / L, H 2 O 1L, pH 7.0 basal medium, inoculum size 2.0%, 180r / min, 37°C for 36 hours to obtain the fermentation stock solution.

[0018] Preparation of biodegradable bacterial agent: the first step, stock solution mixing ingredients: Bacillus licheniformis LS04 ( Bacillus licheniformis ), Bacillus pumilus LCOl ( Bacillus pumilus ), Bacillus amyloliquefaciens LC02 ( Bacillus amyloliquefaciens ), Bacillus subtilis LS02 ( Bacillus subtilis ) The 4 kinds of fermentation stock solution are fully mixed according to the volume ratio of 2:2:3:3. The second step, inoculation: inoculate the mixed stock solution in (NH 4 ) 2 SO 4 2.0g / L, NaH 2 PO 4 3.0g / L, 2....

Embodiment 2

[0020] Embodiment 2: Bacillus licheniformis LS04 ( Bacillus licheniformis ), Bacillus pumilus LCOl ( Bacillus pumilus ), Bacillus amyloliquefaciens LC02 ( Bacillus amyloliquefaciens ), Bacillus subtilis LS02 ( Bacillus subtilis ) were inoculated in glucose 10.0g / L, peptone 10.0g / L, CaCl 2 1.5g / L, MgSO 4 1.5g / L, H 2 O 1L, pH 7.0 basal medium, inoculum size 2.0%, 180r / min, 37°C for 36 hours to obtain the fermentation stock solution.

[0021] Preparation of biodegradable bacterial agent: the first step, stock solution mixing ingredients: Bacillus licheniformis LS04 ( Bacillus licheniformis ), Bacillus pumilus LCOl ( Bacillus pumilus ), Bacillus amyloliquefaciens LC02 ( Bacillus amyloliquefaciens ), Bacillus subtilis LS02 ( Bacillus subtilis ) The 4 kinds of fermentation stock solution are fully mixed according to the volume ratio of 2:2:3:3. The second step, inoculation: inoculate the mixed stock solution in (NH 4 ) 2 SO 4 3.0g / L, NaH 2 PO 4 2.0g / L, 2.0%...

Embodiment 3

[0023] Embodiment 3: Bacillus licheniformis LS04 ( Bacillus licheniformis ), Bacillus pumilus LCOl ( Bacillus pumilus ), Bacillus amyloliquefaciens LC02 ( Bacillus amyloliquefaciens ), Bacillus subtilis LS02 ( Bacillus subtilis ) were inoculated in glucose 10.0g / L, peptone 10.0g / L, CaCl 2 1.5g / L, MgSO 4 1.5g / L, H 2 O 1L, pH 7.0 basal medium, inoculum size 2.0%, 180r / min, 37°C for 36 hours to obtain the fermentation stock solution.

[0024] Preparation of biodegradable bacterial agent: the first step, stock solution mixing ingredients: Bacillus licheniformis LS04 ( Bacillus licheniformis ), Bacillus pumilus LCOl ( Bacillus pumilus ), Bacillus amyloliquefaciens LC02 ( Bacillus amyloliquefaciens ), Bacillus subtilis LS02 ( Bacillus subtilis ) The 4 kinds of fermentation stock solution are fully mixed according to the volume ratio of 2:2:3:3. The second step, inoculation: inoculate the mixed stock solution in (NH 4 ) 2 SO 4 4.0g / L, NaH 2 PO 4 1.5g / L, 2....

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Abstract

The invention discloses a preparation method and an application method of a radioactive decontamination strippable film biodegradation bacterial agent. The preparation method comprises the following steps: respectively inoculating bacillus licheniformis LS04, bacillus pumilus LCOl, bacillus amyloliquefaciens LC02 and bacillus subtilis LS02 into a basic culture medium, and fermenting at the speed of 180r / min and the temperature of 37 DEG C for 36 hours to obtain fermentation stock solutions; fully and uniformly mixing the four fermentation stock solutions according to the volume ratio of 2: 2: 3: 3; inoculating the mixed stock solution into the fermentation culture solution according to the inoculation amount of 10.0% of the total amount; and ventilating, stirring and fermenting at 28 DEG C for 48 hours until the content of microorganisms in the culture solution is not less than 4.0 * 10 < 8 > CFU / mL, thereby obtaining the radioactive decontamination strippable film biodegradable microbial agent. After the radioactive decontamination peelable film is used and peeled, 10%-15% by weight of the additive is added and uniformly mixed, natural stockpiling is performed for 30-60 days, and the degradation rate is larger than or equal to 90%.

Description

technical field [0001] The invention relates to a radioactive decontamination strippable film biodegradable bacterial agent, which belongs to the technical field of radioactive waste treatment and disposal in the field of nuclear science and technology. Background technique [0002] Since the 1980s, with the decommissioning of a large number of nuclear facilities and the treatment of radioactive waste at home and abroad, the surface decontamination technology of peelable decontamination membranes has been developed and formed. The decontamination of the peelable decontamination film is to spray or brush the decontamination agent on the surface of the nuclear facility substrate. The force and adhesion are concentrated on the membrane. After the membrane liquid dries, a thin film can be formed on the surface of the facility substrate, and then the decontamination membrane is peeled off, so as to achieve the purpose of decontamination, sealing and fixing pollutants. Compared w...

Claims

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Application Information

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IPC IPC(8): C12N1/20G21F9/18C12R1/10C12R1/125C12R1/07
Inventor 张宇习海玲张丽李占国罗学刚赖金龙
Owner SOUTHWEAT UNIV OF SCI & TECH
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