Glucose detection reaction liquid and cell sap glucose content detection method

Abstract

The invention discloses a method for detecting the content of glucose in cell sap. The method comprises the following steps: a, adding a buffer agent, a cell sap sample and a standard substance into a dilution plate; b, preparing a glucose detection reaction solution; c, adding a glucose detection reaction solution into the detection plate; d, detecting the glucose concentration of the cell sap sample by a microplate reader, and calculating the glucose content of the cell sap sample; according to the method, glucose content detection can be completed by using a small amount of cell sap sample in the cell culture process, pretreatment work such as centrifugation is not needed, and detection steps are simplified; the determination can be completed by a microplate reader, and the operation is simple; meanwhile, a mixing device is adopted when the glucose detection reaction liquid is prepared, so that the mixing efficiency can be improved; the detection efficiency is further improved, and yield detection is facilitated.

Description

technical field [0001] The invention belongs to the technical field of detection of glucose content in cell fluid, and in particular relates to a detection method for glucose content in cell fluid. Background technique [0002] Glucose, an organic compound with the molecular formula C6H12O6, is the most widely distributed and most important monosaccharide in nature. Glucose plays an important role in the field of biology. It is the energy source and metabolic intermediate product of living cells, and it is also the main energy supply material of cells. In cell culture, especially in batch-fed experiments, the monitoring and timely supplementation of glucose is the basis for maintaining cell survival. [0003] At present, the glucose content in laboratory cells is mainly detected by ultraviolet spectrophotometer or automatic biochemical analyzer. The detection principle is mainly based on glucose oxidase method, hexokinase method, glucose oxygen electrode method, etc. Altho...

AI Technical Summary

Problems solved by technology

Although the instrument detection is highly automated and convenient, it is difficult to complete the detection of a large number of cell fluid samples at the same time due to the limited throughput of the instrument.

In addition, the use of biochemical analyzers to detect Glucose is limited by the instrument, and supporting reagents, calibrators, and quality controls are required, and the cost per unit cell fluid sample is relatively high

Although the cost of the ultraviolet method is relatively low, it requires a relatively large amount of cell fluid samples, which is not conducive to the use of small volume cell culture.

[0004] The disadvantages of these existing technologies are: the detection cost is high, the amount of cell fluid samples is large, and the detection of a large number of cell fluid samples cannot be completed at the same time, and the use is limited by instruments, consumables, resources, etc.; The cell fluid sample can quickly, accurately and massively complete the reaction solution and detection method for glucose content detection, and the present invention solves such problems;

[0005] Before checking, it is necessary to mix the reaction solution. In the existing technology, the test tube is placed on the microplate, and then the microplate is placed on a shaking machine to shake and mix; but when shaking, due to the shaking range, The direction and speed are single, but the mixing effect of the mixed solution is reduced; at the same time, due to the inertia, the liquid in the test tube will stick to the test tube cover when shaking, resulting in waste of reaction solution

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