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Photoelectric response type nanoparticle composite oriented microfiber, cell-loaded photoelectric stimulation nerve scaffold and preparation method of photoelectric response type nanoparticle composite oriented microfiber and cell-loaded photoelectric stimulation nerve scaffold

A nanoparticle and photoelectric response technology, which is applied in the fields of biomedical materials and biomedical engineering, can solve the problems that are difficult to apply to nerve regeneration research, unfavorable cell growth, and limited cytocompatibility, and achieve good application prospects of neural tissue engineering. The effects of enhanced outgrowth and functional expression, simple and efficient preparation method

Active Publication Date: 2022-04-19
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method of preparing photoelectrically stimulated nerve scaffolds realizes the construction of oriented topology and promotes the oriented growth of cells, due to the limited cytocompatibility of P3HT and PCL, P3HT is extremely hydrophobic, and its cell adhesion sites are limited. detrimental to cell growth
In addition, P3HT raw materials are expensive, and electrospinning technology requires a large amount of raw materials, so its application in neural scaffold materials is limited
[0005] In summary, currently reported photoelectric stimulation nerve scaffolds are poor in biocompatibility, complicated in material preparation process, high in preparation cost or lack of oriented microstructure. and other defects, making it difficult to apply to nerve regeneration research

Method used

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  • Photoelectric response type nanoparticle composite oriented microfiber, cell-loaded photoelectric stimulation nerve scaffold and preparation method of photoelectric response type nanoparticle composite oriented microfiber and cell-loaded photoelectric stimulation nerve scaffold
  • Photoelectric response type nanoparticle composite oriented microfiber, cell-loaded photoelectric stimulation nerve scaffold and preparation method of photoelectric response type nanoparticle composite oriented microfiber and cell-loaded photoelectric stimulation nerve scaffold
  • Photoelectric response type nanoparticle composite oriented microfiber, cell-loaded photoelectric stimulation nerve scaffold and preparation method of photoelectric response type nanoparticle composite oriented microfiber and cell-loaded photoelectric stimulation nerve scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In this example, the steps for preparing bioactive photoelectrically responsive nanoparticle composite oriented microfibers are as follows:

[0044] (1) Preparation of hydrophilic P3HT nanoparticles

[0045] At room temperature, P3HT was dissolved in chloroform to form a first solution with a P3HT concentration of 5 mg / mL; SDS was dissolved in deionized water to form a second solution with a concentration of 5 mmol / L, and a cosurfactant was added to the second solution. Propanol forms the third solution (the volume ratio of co-surfactant to the second solution is 1:100); each takes 1 mL of the first solution and the third solution, mixes them, disperses ultrasonically at 125W for 20 minutes, and then evaporates at 65°C for 10 minutes Chloroform forms the P3HT nanoparticle aqueous dispersion, the resulting solution is lyophilized and weighed to determine the product concentration, and a certain amount of deionized water is added to make the nanoparticle concentration 0.2...

Embodiment 2

[0053] In this example, the steps for preparing bioactive photoelectrically responsive nanoparticle composite oriented microfibers are as follows:

[0054] (1) Preparation of hydrophilic P3HT nanoparticles

[0055] At room temperature, P3HT was dissolved in chloroform to form a first solution with a P3HT concentration of 10 mg / mL; SDS was dissolved in deionized water to form a second solution with a concentration of 10 mmol / L, and a cosurfactant was added to the second solution. Propanol forms the third solution (the volume ratio of co-surfactant to the second solution is 1:100); each takes 1 mL of the first solution and the third solution, mixes them, ultrasonically disperses at 125W for 30 minutes, and then evaporates at 65°C for 15 minutes Chloroform forms the P3HT nanoparticle aqueous dispersion, the resulting solution is lyophilized and weighed to determine the product concentration, and a certain amount of deionized water is added to make the nanoparticle concentration 0...

Embodiment 3

[0063] In this example, the steps for preparing bioactive photoelectrically responsive nanoparticle composite oriented microfibers are as follows:

[0064] (1) Preparation of hydrophilic P3HT nanoparticles

[0065] At room temperature, P3HT was dissolved in chloroform to form a first solution with a P3HT concentration of 5 mg / mL; SDS was dissolved in deionized water to form a second solution with a concentration of 5 mmol / L, and a cosurfactant was added to the second solution. Propanol forms the third solution (the volume ratio of co-surfactant to the second solution is 1:100); each takes 1 mL of the first solution and the third solution, mixes them, disperses ultrasonically at 125W for 20 minutes, and then evaporates at 65°C for 10 minutes Chloroform forms the P3HT nanoparticle aqueous dispersion, the resulting solution is lyophilized and weighed to determine the product concentration, and a certain amount of deionized water is added to make the nanoparticle concentration 0.2...

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Abstract

The invention discloses a photoelectric response type nanoparticle composite oriented microfiber with biological activity and a preparation method thereof. The composite oriented microfiber is composed of a PCL microfiber, collagen and hydrophilic P3HT nanoparticles, wherein the surface of the PCL microfiber is coated with the collagen and the hydrophilic P3HT nanoparticles in sequence. The hydrophilic P3HT nanoparticles are obtained by performing surface modification on a semiconductor polymer P3HT through lauryl sodium sulfate (SDS) and normal propyl alcohol. The microfiber is endowed with biological activity and photoelectric responsiveness while the microstructure of the microfiber is not changed. The invention further discloses a cell-loaded photoelectric stimulation nerve scaffold, the cell-loaded photoelectric stimulation nerve scaffold is obtained by inoculating cells on the surface of a formed photoelectric response type nanoparticle composite orientation microfiber material, the cell-loaded photoelectric stimulation nerve scaffold has high orientation, and the orientation structure can induce orientation growth of nerve cells and simulate arrangement of neurons in natural nerve cells in the direction of nerve fibers.

Description

technical field [0001] The invention belongs to the technical fields of biomedical materials and biomedical engineering, and relates to a bioactive photoelectric-responsive nano particle composite oriented microfiber, an oriented nerve support capable of photoelectric stimulation and its preparation. Background technique [0002] Natural nerve tissue has a multi-level directional structure, which is composed of oriented nerve bundles, and nerve bundles are composed of micron-scale nerve fibers. In nerve fibers, Schwann cells grow in a directional arrangement to form myelin sheath, and neurons Axons grow oriented along the myelin sheath. [0003] In nerve tissue, electrical signal is the main medium of communication between nerve cells, so it plays a vital role in the growth, differentiation and functional expression of nerve cells. The self-recovery ability of nerve tissue after damage is extremely limited. In recent years, a large number of literatures have reported the pr...

Claims

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Application Information

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IPC IPC(8): D06M15/15D06M15/63C12N5/079D06M101/32
CPCD06M15/15D06M15/63C12N5/0618D06M2101/32C12N2533/30C12N2533/54C12N2529/10C12N2529/00
Inventor 范红松吴承恒陈素萍陈露孙静罗红蓉
Owner SICHUAN UNIV
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