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Exosome/honey compounded drug-loaded sustained-release microspheres and preparation method thereof

A technology for slow-release microspheres and exosomes, which can be used in pharmaceutical formulations, medical formulations with inactive ingredients, and medical formulations containing active ingredients, etc. , biocompatibility and immunogenicity need to be further improved to achieve the effect of improving biocompatibility and immunogenicity, maintaining drug delivery function, excellent drug loading function and drug sustained release function

Pending Publication Date: 2022-04-22
BIOISLAND LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the two are used as carrier materials, it is easy to cause adverse effects on the physiological balance of the microenvironment of the organism, and at the same time, when used on body surface wounds, it is not conducive to wound healing.
There is a method to prepare drug-loaded microspheres by adding honey to polylactic-co-glycolic acid (PLGA) or polylactic acid (PLA), but its biocompatibility and immunogenicity still need to be further improved

Method used

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  • Exosome/honey compounded drug-loaded sustained-release microspheres and preparation method thereof
  • Exosome/honey compounded drug-loaded sustained-release microspheres and preparation method thereof
  • Exosome/honey compounded drug-loaded sustained-release microspheres and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0040]In a specific example, the preparation method of exosomes includes the following steps:

[0041] The supernatant of the culture medium of neural stem cells and / or mesenchymal stem cells is collected, impurities are removed and concentrated, the obtained concentrated solution is extracted with an exosome extraction column, and the obtained extracted solution is concentrated.

[0042] Further, concentrating (including the concentrating step after the impurity removal step and / or concentrating the extract) refers to concentrating with an ultrafiltration membrane with a molecular weight cut-off of 50-150 kD. The exosomes obtained by concentrating the ultrafiltration membrane can effectively remove impurity components, and the enriched exosomes are conducive to the effective loading of drugs, and the drug loading capacity is relatively high. Specifically, the molecular weight cut-off of the ultrafiltration membrane includes but is not limited to the following molecular weight...

Embodiment 1

[0073] In this example, a drug-loaded sustained-release microsphere compounded with exosomes / honey, the preparation method is as follows:

[0074] (1) After taking out PLGA from the refrigerator, let it stand for about 10 minutes to evaporate the water on the surface of the bag, weigh 0.3g of PLGA, then take a 10mL measuring cylinder, and measure 10mL of N,N-dimethylformamide, Pour the weighed PLGA, 0.5mL honey and 200uL exosomes containing norfloxacin (the concentration of exosomes containing norfloxacin obtained is 50ug / mL) into the above N,N-dimethyl In the formamide organic solvent, after stirring for 1.5 hours, an electrostatic spray solution was obtained, which was placed at room temperature for later use;

[0075] (2) Perform electrostatic spraying on the electrostatic spray liquid: turn on the electrostatic spray machine, after preheating for 30 minutes, adjust the positive voltage to 20kV, the negative voltage to -1kV, and the nozzle and spray receiving distance to be...

Embodiment 2

[0078] In this example, a drug-loaded sustained-release microsphere compounded with exosomes / honey, the preparation method is as follows:

[0079](1) After taking out the PLGA material from the refrigerator, let it stand for about 10 minutes to evaporate the water on the surface of the bag, weigh 0.3g of PLGA, then take a 10mL measuring cylinder, and measure N,N-dimethylformamide / Each 5mL of dichloromethane, the weighed PLGA, 0.5mL of honey and 200uL exosomes containing norfloxacin (the concentration of exosomes containing norfloxacin obtained is 50ug / mL) were poured into the above N , in N-dimethylformamide / dichloromethane organic solvent, after stirring for 1.5h, an electrostatic spray solution was obtained, which was placed at room temperature for subsequent use;

[0080] (2) Perform electrostatic spraying on the electrostatic spray liquid: turn on the electrostatic spray machine, after preheating for 30 minutes, adjust the positive voltage to 20kV, the negative voltage to...

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Abstract

The invention relates to an exosome / honey composite drug-loaded sustained release microsphere and a preparation method thereof. The exosome / honey compounded drug-loaded sustained-release microsphere comprises an inner core and a carrier material wrapping the surface of the inner core, the inner core comprises an exosome loaded with a medicine and honey; the carrier material is a polylactic acid-glycolic acid copolymer and / or polylactic acid. The drug-loaded sustained-release microsphere has better biocompatibility and immunogenicity, can effectively carry drugs, and has a very good drug sustained-release effect.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to an exosome / honey composite drug-loaded sustained-release microsphere and a preparation method thereof. Background technique [0002] Exosomes refer to small membrane vesicles containing complex RNA and proteins, which were first discovered in sheep reticulocytes in 1983, and Johnstone named them "exosomes" in 1987. A variety of cells can secrete exosomes under normal and pathological conditions, which are mainly derived from the multivesicular bodies formed by the invagination of intracellular lysosomal particles, and are released into the extracellular matrix after the fusion of the multivesicular outer membrane and the cell membrane middle. At present, exosomes are widely used as pharmacologically active substances in the treatment of various diseases due to their stable properties, easy reaction, non-immunogenicity, easy acquisition, and easy transformatio...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/46A61K47/34A61K45/00A61K31/496A61P31/04
CPCA61K9/5068A61K9/5031A61K9/5063A61K45/00A61K31/496A61P31/04Y02A50/30
Inventor 杨军政罗浩郑辉孙佳琦苗晓莉
Owner BIOISLAND LAB
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