Device and method for dispersing cell mass

Abstract

The invention relates to a device for dispersing a cell mass by utilizing a liquid flow shearing force, which comprises at least one dispersing structure component, and the dispersing structure component comprises at least one nozzle. The device can be used in a closed liquid path system, the cell mass is dispersed into single cell distribution through the shearing force provided by the liquid flow, the efficiency is higher than that of blowing away the cell mass through a pipette in an open environment, and the risk that a cell sample is polluted is greatly reduced.

Description

technical field [0001] The invention relates to a device for dispersing cell agglomerates, more specifically, the invention relates to a device for dispersing cell agglomerates into individual cells in a closed pipeline by utilizing the shearing force of liquid flow. Background technique [0002] The T cells activated by PBMC are in agglomerated / agglomerated state, but in order to achieve the highest electrotransfection efficiency during electrotransfection, it is necessary to form a single cell suspension. The traditional method often requires the experimenter to hold the pipette gun and the test tube or centrifuge tube to continuously press and blow off / flush away, and use the tip with a wide top and a narrow bottom to provide corresponding shearing force, and the cell mass passing through the tip It is time-consuming and labor-intensive to disperse the block several times to make it disperse into single cells; although the above-mentioned operations are completed on the u...

AI Technical Summary

Problems solved by technology

But: first, the beneficial effect of the whole device only shows that "the cell suspension can be blown and dispersed in a closed environment, and the cell suspension can be uniformly mixed", and it does not clearly indicate that a single-cell suspension can be finally obtained; second, An important application of this device is to enable the cell suspension to be directly used for subsequent spinner bottle culture and large-scale bioreactor culture, so on the whole, this invention is actually a cell culture device, and "cell blowing" is only one of them. One link; third, the whole device includes a first container, a second container, a third container, a first flexible pipe, a second flexible pipe, a third flexible pipe, a fourth flexible pipe, a fifth flexible pipe and some peristaltic pumps, The structure of the device and the operation process are relatively complicated; four, the whole device is still in an open working environment during the actual operation, even if the first container, the second container and the third container are respectively equipped with air filters, but in the For example, during operations such as adding 1L to 10L of cell culture medium to the third container 3 and adding digested cell suspension to the first container 1, there is still a risk of contamination of the cell fluid in the tube.

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