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Biocontrol bacterial liquid for storage period of Yuluxiang pears as well as preparation method and application of biocontrol bacterial liquid

A bio-control bacteria and fragrant pear technology is applied in the field of bio-control bacteria liquid and its preparation during storage of Yulu fragrant pears, which can solve the problems such as drug resistance, pollution, microbial infection and disease caused by pathogenic bacteria, and achieve the goal of inhibiting harmful bacteria. The effect of breeding, ensuring long-term storage, and slowing down the decay process

Active Publication Date: 2022-04-29
山西农业大学食品科学与工程学院(山西省农业科学院农产品贮藏保鲜研究所)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of its thin peel and rich nutrients, it is susceptible to microbial infection and disease during storage and transportation. Common diseases include pear scab, pear flesh browning, pear ring spot, pear brown rot, pear Soft rot, etc., have seriously affected the commodity value of the fruit
[0003] At present, post-harvest pear diseases have become an important factor restricting the healthy development of the Yulu pear industry. In actual production, chemical pesticides are generally used to prevent and control, but excessive use of pesticides will lead to drug resistance of pathogenic bacteria, and bring pesticide residues and environmental pollution. , bringing great threats and risks to the green and safe production of pome fruit
Alternaria fungi are a group of fungi widely distributed in the natural environment. Some species of this genus can cause a variety of important crop diseases, seriously endanger crop production and cause huge losses.
In addition, the toxins produced by these Alternaria are also accumulated in agricultural products, endangering the food safety of agricultural products

Method used

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  • Biocontrol bacterial liquid for storage period of Yuluxiang pears as well as preparation method and application of biocontrol bacterial liquid

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Experimental program
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preparation example Construction

[0021] The present invention also provides a method for preparing biocontrol bacterial liquid during storage of Yulu Fragrant Pear, comprising the following steps: fermenting liquid of Bacillus subtilis, fermentation liquid of Bacillus megaterium, fermentation liquid of Sphingomonas noni, glucose The solution is mixed with 2,6-di-tert-butyl-p-cresol solution to obtain the biocontrol bacteria liquid during storage of Yulu pear.

[0022] The invention also provides the application of the biocontrol bacteria liquid during storage of Yulu fragrant pears in the biological control of Yulu fragrant pears during storage.

[0023] In the present invention, the application process is preferably: before the Yulu fragrant pear is stored, the Yulu fragrant pear is immersed in the biocontrol bacteria solution.

[0024] In the present invention, the immersion time is preferably 10-50 s, more preferably 20-40 s, even more preferably 38 s.

Embodiment 1

[0027] 5 parts of Bacillus subtilis fermentation broth, 8 parts of Bacillus megaterium fermentation broth, 10 parts of Noni Sphingomonas fermentation broth, 50 parts of glucose solution with a mass concentration of 5%, and 3 parts of a molar concentration of 0.2mmol L -1 2,6-di-tert-butyl-p-cresol solution mixed to obtain the biocontrol bacteria liquid during storage of Yulu Fragrant Pear, wherein the concentration of live bacteria in the Bacillus subtilis fermentation liquid was 1×10 6 CFU / mL, the concentration of live bacteria in the Bacillus megaterium fermentation broth is 1×10 9 CFU / mL, the concentration of live bacteria in the Noni Sphingomonas fermentation broth is 1×10 8 CFU / mL, the Bacillus subtilis in the Bacillus subtilis fermentation broth is ACCC 60364, the Bacillus megaterium in the Bacillus megaterium fermentation fluid is ACCC 02745, and the Bacillus megaterium in the Noni Sphingomonas fermentation fluid Sphingomonas noni is ACCC 60370.

Embodiment 2

[0029] 6 parts of Bacillus subtilis fermentation broth, 10 parts of Bacillus megaterium fermentation broth, 13 parts of Noni Sphingomonas fermentation broth, 55 parts of glucose solution with a mass concentration of 6%, and 4 parts of a molar concentration of 0.3mmol L -1 2,6-di-tert-butyl-p-cresol solution mixed to obtain the biocontrol bacteria liquid during storage of Yulu Fragrant Pear, wherein the concentration of live bacteria in the Bacillus subtilis fermentation liquid was 1×10 6 CFU / mL, the concentration of live bacteria in the Bacillus megaterium fermentation broth is 1×10 9 CFU / mL, the concentration of live bacteria in the Noni Sphingomonas fermentation broth is 1×10 8 CFU / mL, the Bacillus subtilis in the Bacillus subtilis fermentation broth is ACCC 60364, the Bacillus megaterium in the Bacillus megaterium fermentation fluid is ACCC 02745, and the Bacillus megaterium in the Noni Sphingomonas fermentation fluid Sphingomonas noni is ACCC 60366.

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Abstract

The invention belongs to the technical field of biocontrol bacterial liquid, and provides biocontrol bacterial liquid used in the storage period of Yuluxiang pears as well as a preparation method and application of the biocontrol bacterial liquid. The biocontrol bacterial liquid provided by the invention is prepared from the following raw materials in parts by weight: 5 to 7 parts of bacillus subtilis fermentation liquid, 8 to 12 parts of bacillus megatherium fermentation liquid, 10 to 15 parts of morinda citrifolia sphingomonas fermentation liquid, 50 to 60 parts of glucose solution and 3 to 5 parts of 2, 6-butylated hydroxytoluene solution. The biocontrol bacterial liquid disclosed by the invention is reasonable in proportion, all the components are synergistically matched, and breeding of harmful bacteria in the storage period of the Yuluxiang pears can be effectively inhibited, so that the rotting process of the Yuluxiang pears is slowed down, and long-term storage of the Yuluxiang pears is ensured. Technical guarantee is provided for biological prevention and control in the storage period of the Yulu bergamot pears.

Description

technical field [0001] The invention relates to the technical field of biocontrol bacteria liquid, in particular to a biocontrol bacteria liquid during storage of Yulu fragrant pears, a preparation method and application thereof. Background technique [0002] Yulu Fragrant Pear is the primary fruit for large-scale production by fruit farmers in Shanxi Province. It has many advantages such as thin skin, fine meat, small core, high edible rate, and high sugar content. It is recognized as "China's No. 1 Pear" by experts in the national pear industry system. . However, because of its thin peel and rich nutrients, it is susceptible to microbial infection and disease during storage and transportation. Common diseases include pear scab, pear flesh browning, pear ring spot, pear brown rot, pear Soft rot, etc. have seriously affected the commodity value of the fruit. [0003] At present, post-harvest pear diseases have become an important factor restricting the healthy development ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23B7/155A23B7/154C12R1/125C12R1/11C12R1/01
CPCC12N1/20A23B7/155A23B7/154A23V2002/00A23V2200/10A23V2250/61Y02A50/30
Inventor 张立新张晓宇王丽英秦一帆侯亚茹陈会燕陈园园
Owner 山西农业大学食品科学与工程学院(山西省农业科学院农产品贮藏保鲜研究所)