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Method for efficiently recovering protein in shrimp shells

A shrimp shell and protein technology, which is applied in the field of protein recovery, can solve the problems of high cost, large consumption of protease, and easy backlog, etc., and achieve the effect of reducing dosage and improving protein yield

Pending Publication Date: 2022-04-29
YANGZHOU RIXING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] What is incompatible with the industrial cluster is that the development of the supporting processing industry lags behind, the amount of waste is large, and it is easy to backlog and form secondary pollution; the traditional treatment method mainly uses the chitin component selectively, and the remaining organic waste accounts for more than 55%. Biomass is wasted in the process of processing, and high value has not been realized. It is urgent to develop green and biological deep processing technology, develop new high value deep processing products, and build a new industrial model of full value utilization of resources
[0004] The by-products of shrimp processing mainly include shrimp heads and shells, which contain chitin, protein, fat, organic calcium and other components. The current enzymatic hydrolysis treatment of shrimp shells to recover protein is relatively simple, the recovery rate of protein is not high, and it causes protease consumption The quantity is large, the cost is high, and it cannot be industrialized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for efficiently recovering protein from shrimp shells comprises the following steps:

[0024] (1) repeatedly washing shrimp shells with running water, and drying; Then crushing to a particle size of 0.125-0.15mm to obtain shrimp shell primary powder;

[0025] (2) adding the shrimp shell primary powder and water into an ultrasonic stirrer for ultrasonic treatment for 1.0h under the condition of 20KW power, and uniformly mixing the shrimp shell primary powder and water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder and water is 0.5: 1 during ultrasonic stirring and mixing; Directly transferring the slurry obtained above into a sand mill, sanding at a sanding rate of 1500r / min for 2 hours, and drying to obtain shrimp shell powder;

[0026] (3) adding water and alkaline protease into shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of shrimp shell powder to water is 0.5:1, and the dosage of alkaline protease is 0.5 IU / g; ...

Embodiment 2

[0029] A method for efficiently recovering protein from shrimp shells comprises the following steps:

[0030] (1) repeatedly washing shrimp shells with running water, and drying; Then crushing to a particle size of 0.125-0.15mm to obtain shrimp shell primary powder;

[0031] (2) adding the shrimp shell primary powder and water into an ultrasonic stirrer for ultrasonic treatment for 1.0h under the condition of 50KW power, and uniformly mixing the shrimp shell primary powder and water to obtain slurry, wherein the mass ratio of the shrimp shell primary powder and water is 0.8: 1 during ultrasonic mixing; Directly transferring the slurry obtained above into a sand mill, sanding at a sanding speed of 1500r / min for 3.5h hours, and drying to obtain shrimp shell powder;

[0032] (3) adding water and alkaline protease into shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of shrimp shell powder to water is 0.5:1, and the dosage of alkaline protease is 0.5 IU / g; After hyd...

Embodiment 3

[0035]A method for efficiently recovering protein from shrimp shells comprises the following steps:

[0036] (1) repeatedly washing shrimp shells with running water, and drying; Then crushing to a particle size of 0.125-0.15mm to obtain shrimp shell primary powder;

[0037] (2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and ultrasonically mixing the shrimp shell primary powder and water for 2.5h under the power of 85KW to obtain slurry, wherein the mass ratio of the shrimp shell primary powder and water is 1.0: 1 during ultrasonic mixing; The above-mentioned slurry was directly transferred into a sand mill, sand-milled for 4.0h at a sand-milling rate of 3000r / min, and dried to obtain shrimp shell powder;

[0038] (3) adding water and alkaline protease into shrimp shell powder for hydrolysis for 1.0h, wherein the mass ratio of shrimp shell powder to water is 0.5:1, and the dosage of alkaline protease is 0.5 IU / g; After hydrolysis, the temperature i...

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Abstract

The invention discloses a method for efficiently recovering protein in shrimp shells, and belongs to the technical field of protein recovery. The method comprises the following steps: (1) repeatedly washing shrimp shells with running water, and drying; then crushing the shrimp shells until the particle size is 0.125-0.150 mm to obtain shrimp shell primary powder; (2) adding the shrimp shell primary powder and water into an ultrasonic stirrer, and uniformly mixing to obtain slurry; adding the slurry into a sand mill for sanding treatment, and after sanding is finished, drying to obtain shrimp shell powder; (3) adding water and alkaline protease into the shrimp shell powder for hydrolysis, raising the temperature to 100 DEG C after hydrolysis, heating in a water bath for enzyme inactivation, and cooling to obtain protein hydrolysate; and (4) carrying out spray drying on the obtained protein hydrolysate to obtain protein powder. According to the method, the prawn shells are subjected to sanding treatment to obtain prawn shell powder, and then enzyme hydrolysis treatment is performed, so that the dosage of alkaline protease during enzyme hydrolysis can be reduced, and the protein yield is effectively improved.

Description

Technical field [0001] The invention belongs to the technical field of protein recovery, and particularly relates to a method for efficiently recovering protein from shrimp shells. technical background [0002] At present, China has become the world's largest country in the cultivation and processing of all kinds of shrimps, such as Macrobrachium, Penaeus and Crayfish, with 15 million mu of water surface as the main species and a total output of more than 2.5 million tons of shrimps. The shrimp processing level has also been continuously improved, the variety has been continuously enriched, the international competitiveness has been significantly enhanced, and the industrial chain and format have been gradually optimized. However, there are still many problems in the supporting development of by-product resources. [0003] What is incompatible with industrial clusters is that the development of supporting processing industry lags behind, the amount of waste is large, and it is ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 丁振中史劲松邓红兵孙剑龚劲松李瑞怡邱益彬管平海
Owner YANGZHOU RIXING BIO TECH