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Dendrobium officinale extract and preparation method thereof

A technology for extracting iron sheet fenugreek, which is applied in the field of biological active substance extraction and can solve the problems of low yield and purity of polysaccharides.

Active Publication Date: 2022-05-06
浙江长典药物技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, existing methods still have the defects of low polysaccharide yield and purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The dried Tiepi Fengdou is crushed and passed through a 150-mesh sieve to obtain Tiepi Fengdou fine powder. According to the solid-liquid ratio of 1:3 (g / mL), the Tiepifengdou fine powder was mixed with 95% ethanol, and refluxed at 60°C for 8 hours to obtain the Tiepifengdou ethanol dispersion. Hydrotalcite (Mg 6 Al 2 (CO 3 )(OH) 16 4H 2 O, with an average particle size of about 3 μm; CAS: 11097-59-9, Merck Company) was added to the Tiepifengdou ethanol dispersion and mixed well; wherein the weight ratio of hydrotalcite to Tiepifengdou fine powder was 1.4:1 . The solvent was distilled off under reduced pressure to obtain Tiepifengdou hydrotalcite dispersion. Put 500g of Tiepi Fengdou hydrotalcite dispersion in a supercritical extraction tank for supercritical extraction and separation. The supercritical fluid is 15wt% absolute ethanol + 85wt% CO 2 . The extraction and separation conditions are as follows: the extraction pressure is 30MPa, the extraction temperat...

Embodiment 2

[0031] The dried Tiepi Fengdou is crushed and passed through a 150-mesh sieve to obtain Tiepi Fengdou fine powder. According to the solid-liquid ratio of 1:3 (g / mL), the Tiepifengdou fine powder was mixed with 95% ethanol, and refluxed at 60°C for 8 hours to obtain the Tiepifengdou ethanol dispersion. Hydrotalcite (Mg 6 Al 2 (CO 3 )(OH) 16 4H 2 O, with an average particle size of about 3 μm; CAS: 11097-59-9, Merck Company) was added to the Tiepifengdou ethanol dispersion and mixed well; wherein, the weight ratio of hydrotalcite to Tiepifengdou fine powder was 1.2:1 . The solvent was distilled off under reduced pressure to obtain Tiepifengdou hydrotalcite dispersion. Put 500g of Tiepi Fengdou hydrotalcite dispersion in a supercritical extraction tank for supercritical extraction and separation. The supercritical fluid is 20wt% absolute ethanol + 80wt% CO 2 . The extraction and separation conditions are as follows: the extraction pressure is 30MPa, the extraction tempera...

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Abstract

The preparation method comprises the following steps: putting a dendrobium candidum hydrotalcite dispersion into a supercritical extraction tank, and carrying out supercritical extraction separation; further purifying the crude product by using a cellulose column, sequentially carrying out gradient elution by using distilled water and salt solutions with different concentrations, and collecting and combining eluents; the dendrobium candidum extract is high in polysaccharide yield, high in DPPH free radical scavenging rate and high in polysaccharide purity.

Description

technical field [0001] The invention belongs to the technical field of extraction of biological active substances; it relates to a Tiepifengdou extract and a preparation method thereof; more specifically, it relates to a method for extracting Tiepifengdou biological active substances using supercritical technology and the Tiepifengdou obtained by the method Fengdou Bioactives. Background technique [0002] Tiepi Fengdou is the dried stem of Dendrobium officinale. The medicinal uses of Tiepi Fengdou are recorded in many classics of traditional Chinese medicine. Tiepi Fengdou has the effect of benefiting the stomach and promoting body fluid, nourishing yin and clearing away heat. It is used for yin deficiency and body fluid deficiency, dry mouth and polydipsia, insufficient stomach yin, lack of food and vomiting, persistent deficiency and heat after illness, hyperactivity of fire due to yin deficiency, and fatigue and heat due to bone steaming. , dim eyesight, muscle and bon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61P19/08
CPCA61K36/8984A61P19/08A61K2800/805A61K2800/84Y02P20/54
Inventor 王初李繁荣陈启宏
Owner 浙江长典药物技术开发有限公司