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Extraction method of polylysine

A polylysine, extraction method technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as limiting the development and application of polylysine, and achieve low extraction cost, purity and recovery. High rate and simple method

Pending Publication Date: 2022-05-06
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Polylysine is mostly obtained through the fermentation of Streptomyces albicans. In recent years, the hot spot is the breeding of high-yield strains and the optimization of fermentation process. However, with the gradual improvement of domestic polylysine fermentation level, the extraction process has gradually become The main obstacle that severely limits the development and application of polylysine

Method used

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  • Extraction method of polylysine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Take 1L of fermentation broth and centrifuge it at a speed of 8000r / min for 30min to remove mycelium.

[0041] 2. The supernatant was adjusted to pH 4.0, maintained at 65°C for 20 minutes, and centrifuged at 8000r / min for 20 minutes to remove protein flocs.

[0042] 3. The clarified fermented liquid obtained from pretreatment was ion-exchanged with weakly acidic cation exchange resin D152, wherein the adsorption pH was 7.5, the resin dosage was 160 g / L, the adsorption time was 15 hours, and the adsorption temperature was 25°C.

[0043] 5. After the resin is obtained by filtration, perform desorption. The desorption HCL concentration is 0.2 mol / L, the desorption time is 10 hours, and the desorption temperature is 30°C.

[0044] 6. Concentrate the ion-exchange solution 5 times, and then decolorize it with activated carbon, the decolorization pH is 4.0, the amount of activated carbon is 5%, and the decolorization is 75 minutes at 90°C.

[0045] 7. Filter paper to remov...

Embodiment 2

[0049] 1. Take 1L of fermentation broth and centrifuge it at a speed of 8000r / min for 30min to remove mycelium.

[0050] 2. The supernatant was adjusted to pH 4.0, maintained at 65°C for 20 minutes, and centrifuged at 8000r / min for 20 minutes to remove protein flocs.

[0051] 3. The clarified fermented liquid obtained from the pretreatment was ion-exchanged with weakly acidic cation exchange resin D152, wherein the adsorption pH was 8, the resin dosage was 150 g / L, the adsorption time was 10 h, and the adsorption temperature was 25°C.

[0052] 5. After the resin is obtained by filtration, perform desorption. The desorption HCL concentration is 0.2 mol / L, the desorption time is 10 hours, and the desorption temperature is 30°C.

[0053] 6. Concentrate the ion-exchange solution 5 times, and then decolorize it with activated carbon, the decolorization pH is 4.0, the amount of activated carbon is 5%, and the decolorization is 75 minutes at 90°C.

[0054] 7. Filter paper to remove ac...

Embodiment 3

[0058] 1. Centrifuge the fermentation broth at a speed of 8000r / min for 30min to remove the mycelium.

[0059] 2. The supernatant was adjusted to pH 4.0, maintained at 65°C for 20 minutes, and centrifuged at 8000r / min for 20 minutes to remove protein flocs.

[0060] 3. The clarified fermented liquid obtained from the pretreatment was ion-exchanged with weakly acidic cation exchange resin D152, wherein the adsorption pH was 7, the resin dosage was 180g / L, the adsorption time was 20h, and the adsorption temperature was 25°C.

[0061] 5. After the resin is obtained by filtration, perform desorption. The desorption HCL concentration is 0.2 mol / L, the desorption time is 10 hours, and the desorption temperature is 30°C.

[0062] 6. Concentrate the ion-exchange solution 5 times, and then decolorize it with activated carbon, the decolorization pH is 4.0, the amount of activated carbon is 5%, and the decolorization is 75 minutes at 90°C.

[0063] 7. Filter paper to remove activated ca...

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Abstract

The invention belongs to the field of biochemical engineering, and relates to an extraction method of polylysine. The method comprises the following steps: 1) centrifuging fermentation liquor of streptomyces albus for producing polylysine, and removing mycelia to obtain supernate; (2) adjusting the pH value of the obtained supernate, continuously centrifuging, and removing protein floccules to obtain clear fermentation liquor; (3) carrying out ion exchange on clarified fermentation liquor obtained by pulping through weakly acidic cation exchange resin D152, and filtering; 4) desorbing the filtered resin to obtain an ion exchange liquid; 5) concentrating the ion exchange liquid, and decolorizing with activated carbon; performing suction filtration with filter paper to remove activated carbon, precipitating filtrate with ethanol, and dissolving and precipitating with a small amount of water; and 6) after the organic solvent is completely volatilized, feeding residues for freeze drying to obtain powder, namely the polylysine. The method is simple and easy to implement, and the purity and the recovery rate of polylysine can be improved.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to a method for extracting polylysine, which can improve the purity and recovery rate of polylysine. Background technique [0002] Polylysine has the advantages of broad antibacterial spectrum, strong water solubility, good thermal stability, biodegradability, safety and non-toxicity, etc. The demand for polylysine is increasing year by year. [0003] Polylysine is mostly obtained through the fermentation of Streptomyces albicans. In recent years, the hot spot is the breeding of high-yield strains and the optimization of fermentation process. However, with the gradual improvement of domestic polylysine fermentation level, the extraction process has gradually become The main obstacle seriously limits the development and application of polylysine. The quality of the extraction process directly determines the yield, purity, content, and color quality of the final product. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/46C08G69/10C12P13/02C12R1/47
CPCC08G69/46C08G69/10C12P13/02
Inventor 李南臻曾志刚陈沿言
Owner CHENGDU UNIV
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