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Nucleic acid functionalized MOF (Metal Organic Framework) material as well as preparation and application thereof

A functionalized, nucleic acid technology, applied in the field of nanomaterials and biosensing, to achieve the effect of enhanced fluorescence signal, excellent stimuli-responsive release performance, and broad application prospects

Active Publication Date: 2022-05-06
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation and application of a nucleic acid functionalized MOF material aiming at the existing technical problems

Method used

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  • Nucleic acid functionalized MOF (Metal Organic Framework) material as well as preparation and application thereof
  • Nucleic acid functionalized MOF (Metal Organic Framework) material as well as preparation and application thereof
  • Nucleic acid functionalized MOF (Metal Organic Framework) material as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Taking the determination of ATP as an example to prepare nucleic acid functionalized MOF:

[0037] 1) 488mg ZrCl 4 and 218mg of 2-amino-4,4'-biphenyldicarboxylic acid were added to 30mL of DMF solution, ultrasonically dissolved for 15min to obtain a mixed solution, and then 3.6mL of acetic acid was added to the mixed solution and ultrasonically dissolved for 5min. Then, the reaction mixture was hydrothermally reacted at 100°C for 24 hours to generate UiO-67-NH 2 ; The resulting MOF product (UiO-67-NH 2 ) were washed 3 times with DMF and absolute ethanol respectively, and then vacuum-dried at 50°C (see figure 2 A).

[0038] 2) Mix 250 μL complementary strand DNA (100 μM, 5’COOH-GCAACAACGTACCCAATG3’) with 125 μL EDC (5 mgmL -1 ), 125μL NHS (5mg mL -1 ) stirred and reacted for 20min, then 5mg of the above-mentioned obtained UiO-67-NH 2 Added to the reaction mixture and stirred for 15 hours to obtain modified UiO-67, then washed the modified UiO-67 with PBS (10 mM, p...

Embodiment 2

[0042] Taking the determination of ATP as an example to prepare nucleic acid functionalized MOF:

[0043] 1) 488mg ZrCl 4 and 188 mg of terephthalic acid were added to 30 mL of DMF solution, ultrasonically dissolved for 15 minutes to obtain a mixed solution, and then 3.6 mL of acetic acid was added to the mixed solution for ultrasonically dissolved for 5 minutes. Then, the reaction mixture was hydrothermally reacted at 100°C for 24 hours to generate UiO-66-NH 2 . The resulting MOF product (UiO-66-NH 2 ) were washed 3 times with DMF and absolute ethanol respectively, and then vacuum-dried at 50°C (see image 3 A).

[0044] 2) Mix 250 μL complementary strand DNA (100 μM, 5’COOH-GCAACAACGTACCCAATG3’) with 125 μL EDC (5 mgmL -1 ), 125μL NHS (5mg mL -1 ) stirred and reacted for 20min, then 5mg of the above-mentioned obtained UiO-66-NH 2 Added to the reaction mixture and stirred for 15 hours to obtain modified UiO-66, then washed the modified UiO-66 with PBS (10 mM, pH=7.4), ...

Embodiment 3

[0048] Utilize above-mentioned embodiment to obtain material to carry out the detection of ATP:

[0049] Add 40 μL of the above to 300 μL PBS (10 mM, pH=7.4) to obtain Rho 6G@UiO-66 (250 μgmL -1 ) or Rho 6G@UiO-67 (250μg mL -1 ). Then add 20 μL exonuclease I (800 U mL -1 ) (purchased from Thermo Fisher Scientific) and 40 μL of ATP solution (10 nM). Take 100 μL of reaction solution at different times, centrifuge at 10,000 rpm for 3 minutes, measure the fluorescence intensity of the supernatant at an excitation wavelength of 525 nm and an emission wavelength of 552 nm; record the dye release of Rho 6G@UiO-66 and Rho 6G@UiO-67 obtained from the above examples Curve, at the same time with no exonuclease I, only added ATP sample as a control, no exonuclease I and no added ATP sample as a blank (see Figure 4 ).

[0050] Such as Figure 4 shown. The curve shows that within 30 min, the fluorescence of the sample without adding ATP changes very little. Once the target is prese...

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Abstract

The invention belongs to the field of nano materials and biosensing, and particularly relates to preparation and application of a nucleic acid functionalized MOF (Metal Organic Framework) material. According to the method, 2-amino-4, 4 '-biphenyl dicarboxylic acid and ZrCl4 are subjected to a hydrothermal reaction to generate UiO-67-NH2, single-stranded DNA with carboxyl is connected to the surface of the UiO-67-NH2 through an amidation reaction, an aptamer chain and the single-stranded DNA are partially hybridized, fluorescent dye is wrapped in MOF, a nucleic acid functionalized MOF material is formed, and it is found that the prepared nucleic acid functionalized MOF has excellent stimuli-responsive release performance. The nucleic acid functionalized MOF prepared by the invention has good stability and biocompatibility, and has a wide application prospect in the aspects of drug delivery and biosensing.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biosensing, and in particular relates to a nucleic acid functionalized MOF material and its preparation and application. Background technique [0002] Molecular diagnostics are fundamental tools for the prevention, identification and treatment of many diseases, and the diagnostic process is the basis for effective treatment of various diseases. Therefore, the development of robust diagnostic tools is of paramount importance. So far, various nanomaterials such as magnetic nanoparticles, quantum dots, and gold nanoparticles have been used as signal sensors in biosensing structures. The advantages of these biosensors include strong signal strength, multiple efficient signaling mechanisms, and switchable optical properties of nanoparticles, enabling molecular detection. [0003] Metal-organic frameworks (MOFs) have been widely used in biomedical fields due to their ultra-high surface area, unique po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06G01N33/53G01N33/68C08G83/00C09B67/02B82Y30/00B82Y40/00G01N21/64
CPCC08G83/008G01N33/68G01N33/53C09K11/025C09K11/06C09B67/0097B82Y30/00B82Y40/00G01N21/6428G01N21/6486Y02A50/30
Inventor 张盾王英文戚鹏
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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