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Thermal inactivation clostridium butyricum for preventing or treating asthma and preparation method of thermal inactivation clostridium butyricum

A technology of live Clostridium butyricum and Clostridium butyricum, which is applied in the field of medicine, can solve the problems of unclear anti-asthma efficacy of Clostridium butyricum, and achieve the effect of no risk of infection, good stability and outstanding effect

Pending Publication Date: 2022-05-06
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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Problems solved by technology

Both Clostridium butyricum and heat-inactivated Clostridium butyricum have immune regulation function, but whether heat-inactivated Clostridium butyricum has anti-asthma effect is unclear, and there is no research about the effect of heat-inactivated Clostridium butyricum on the prevention and treatment of asthma. Related Research Reports

Method used

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  • Thermal inactivation clostridium butyricum for preventing or treating asthma and preparation method of thermal inactivation clostridium butyricum
  • Thermal inactivation clostridium butyricum for preventing or treating asthma and preparation method of thermal inactivation clostridium butyricum
  • Thermal inactivation clostridium butyricum for preventing or treating asthma and preparation method of thermal inactivation clostridium butyricum

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Embodiment Construction

[0019] 1. Materials and methods

[0020] 1.1 Material: Clostridium butyricum CGMCC NO: 0313-1 provided by Shandong Kexing Biopharmaceutical Co., Ltd.;

[0021] 1.2 Experimental method

[0022] 1.2.1 Heat inactivation of Clostridium butyricum: First measure the bacterial content of Clostridium butyricum powder, then weigh a certain weight of Clostridium butyricum powder and heat inactivate at 110kpa and 121°C for 20 minutes to obtain heat inactivation Clostridium butyricum. Store at -20°C after naturally cooling to room temperature.

[0023] 1.2.2 Preparation of heat-inactivated Clostridium butyricum bacteria solution: before use, heat-inactivated Clostridium butyricum is added to phosphate buffered saline (PBS) to prepare the bacteria content (the bacteria content before inactivation) for 5X10 8 CFU / ml of suspension. Ultrasonic cell disruptor with 40% power was crushed 4 times to promote dissolution, and the duration of each ultrasound was 15s with an interval of 20s. Th...

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Abstract

The invention discloses heat-inactivated clostridium butyricum for preventing or treating asthma and a preparation method of the heat-inactivated clostridium butyricum, and belongs to the technical field of medicines. The thermal inactivation clostridium butyricum is obtained by performing thermal inactivation on clostridium butyricum powder at high temperature of 110 kpa and 121 DEG C and high pressure for 20 minutes. The heat-inactivated clostridium butyricum can well inhibit airway inflammation and airway mucus secretion in an asthma mouse model experiment, has an immune regulation function, can effectively prevent and treat asthma, can also be used for prevention or treatment of other allergic diseases, and has a good application prospect.

Description

technical field [0001] The invention relates to a heat-inactivated Clostridium butyricum used for preventing or treating asthma and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Currently inhaled glucocorticoids, β-adrenergic receptor agonists, leukotriene receptor antagonists and theophylline and other commonly used asthma treatment drugs can only control symptoms, but cannot cure asthma. These drugs take a long time to treat, have certain side effects, poor compliance, and some patients are not sensitive to these drugs. Therefore, there is an urgent need to find effective new drugs for the prevention and treatment of asthma. [0003] Probiotics are a class of microorganisms that confer health benefits on the host when ingested in appropriate amounts. Probiotics have the effects of regulating immunity, anti-tumor, anti-infection, anti-oxidation, anti-aging, etc., and have been widely used in animal husbandry, f...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61K41/10A61P11/06A61P11/00A61P29/00A61P37/08A61P37/02C12R1/145
CPCC12N1/20A61K41/10A61K35/742A61K9/0053A61K9/0073A61P11/06A61P11/00A61P29/00A61P37/08A61P37/02Y02A50/30
Inventor 李超乾李劳冬
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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