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Method for preparing cycloastragenol by fermenting and converting astragaloside IV by utilizing fusarium oxysporum

A technology of astragaloside IV and cycloastragaloside is applied in the field of medicine and chemical industry, can solve the problems of high conversion rate, pollute the environment, many by-products, etc., and achieve the effects of high conversion rate, low equipment requirements and low cost

Pending Publication Date: 2022-05-06
厦门北化生物产业研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to adopt the method for microbial conversion to overcome the shortcomings of many by-products, high cost, and environmental pollution in the existing chemical and enzymatic preparations of cycloastragenol, and to prepare cycloastragenol by fermentation than the existing microbial fermentation. high rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present embodiment prepares cycloastragenol according to the following steps:

[0025] (1) Seed cultivation

[0026] The preserved strain Fusarium proliferatum was inserted into a 100 mL Erlenmeyer flask filled with wort medium, and cultured at 25° C. and 180 r / min for 48 hours to obtain seed liquid. The composition of wort medium is: glucose 10g / L, peptone 4g / L, yeast extract powder 3g / L, malt powder 10g / L.

[0027] (2) Transformation culture

[0028] The seed liquid cultivated in step (2) was inserted into a 100 mL Erlenmeyer flask containing a fermentation medium according to an inoculum amount of 10%, and cultivated at 25° C. and 180 r / min for 6 days to obtain a fermentation liquid. The composition of the fermentation medium is: astragalus powder 2g / L, peptone 5g / L, yeast powder 2g / L, K2HPO4 1g / L, MgSO4 7H2O 0.5g / L, astragaloside IV substrate 0.2g / L of 50% mass percentage L.

[0029] (3) Separation and purification

[0030] Add 100 mL of water-saturated n-b...

Embodiment 2

[0033] The present embodiment prepares cycloastragenol according to the following steps:

[0034] (1) Seed cultivation

[0035] The strain Fusarium proliferatum was inserted into a 100mL Erlenmeyer flask filled with wort medium, and cultured at 30°C and 180r / min for 36h to obtain seed liquid. The composition of wort medium is: glucose 10g / L, peptone 4g / L, yeast extract powder 3g / L, malt powder 10g / L.

[0036] (2) Transformation culture

[0037] The seed liquid cultivated in step (2) was inserted into a 100 mL Erlenmeyer flask containing a fermentation medium according to a 10% inoculation amount, and cultivated at 30° C. and 180 r / min for 6 days to obtain a fermentation liquid. The composition of the fermentation medium is: astragalus powder 2g / L, peptone 5g / L, yeast powder 2g / L, K2HPO4 1g / L, MgSO4 7H2O 0.5g / L, astragaloside IV substrate 0.2g / L of 50% mass percentage L.

[0038] (3) Separation and purification

[0039] Add 100 mL of water-saturated n-butanol to the fermen...

Embodiment 3

[0042] The present embodiment prepares cycloastragenol according to the following steps:

[0043] (1) Seed cultivation

[0044] The strain Fusarium proliferatum was inserted into a 100mL Erlenmeyer flask filled with wort medium, and cultured at 30°C and 180r / min for 36h to obtain seed liquid. The composition of wort medium is: glucose 10g / L, peptone 4g / L, yeast extract powder 3g / L, malt powder 10g / L.

[0045] (2) Transformation culture

[0046] The seed liquid cultivated in step (2) was inserted into a 100 mL Erlenmeyer flask containing a fermentation medium according to a 10% inoculation amount, and cultivated at 30° C. and 180 r / min for 6 days to obtain a fermentation liquid. The composition of the fermentation medium is astragalus powder 2g / L, peptone 5g / L, yeast powder 2g / L, K2HPO4 1g / L, MgSO4 7H2O 0.5g / L, astragaloside IV substrate 0.2g / L of 100% mass percentage L.

[0047] (3) Separation and purification

[0048]Add 100 mL of water-saturated n-butanol to the ferment...

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Abstract

The invention relates to a method for preparing cycloastragenol by fermenting and converting astragaloside IV by using fusarium palustris 0821 (CGMCC (China General Microbiological Culture Collection Center) No.16482). The invention also relates to a method for preparing the cycloastragenol by using the fusarium palustris 0821). According to the method disclosed by the invention, the cycloastragenol is prepared by utilizing the screened Fusarium proliferum and taking astragaloside as a substrate through fermentation. According to the method, astragaloside is converted to obtain high-purity cycloastragenol by utilizing a Fusarium profermentum fermentation technology, the whole technological process is carried out at normal temperature and normal pressure, required equipment conditions are low, the method is suitable for mass production, the conversion rate of the cycloastragenol reaches 80% or above finally, and the purity of the cycloastragenol reaches 95% or above finally.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, and in particular relates to a method for preparing cycloastragenol by fermenting and transforming astragaloside IV with Fusarium stratumiformis. Background technique [0002] Astragalus is one of the most commonly used medicines in clinical treatment of traditional Chinese medicine. The active ingredients in Astragalus include flavonoids, saponins, polysaccharides and amino acids. Astragaloside IV is a representative compound of astragaloside, which has various functions such as improving immunity, scavenging free radicals, lowering blood pressure and antibacterial. Cycloastragenol is the aglycone of astragaloside, the molecular formula is C30H5OO5, and the relative molecular weight is 490.71. It is the main product of astragaloside IV metabolism in the intestine, and it has the effects of promoting wound healing, promoting nerve cell proliferation, and anti-aging. Moreover, studi...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12P33/06C07J53/00C12R1/77
CPCC12P33/20C12P33/06C07J53/004
Inventor 张鹏潘弘哲陈毅明
Owner 厦门北化生物产业研究院有限公司
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