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Carya illinoensis bacterial fertilizer and application thereof

A technology of thin-shell hickory nuts and bacterial fertilizers, applied in the biological field, can solve problems such as affecting cross-pollination, increasing food safety hazards, and reducing fruit quality

Pending Publication Date: 2022-05-10
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, production is mainly through the use of chemical agents and pesticides to increase yields and control pests and diseases. This method is mostly used in the flowering and fruiting stages. Although it plays a role in controlling diseases, it also affects cross-pollination and reduces fruit production. quality, while increasing food safety hazards

Method used

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  • Carya illinoensis bacterial fertilizer and application thereof
  • Carya illinoensis bacterial fertilizer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Mixed fermentation of sj18 bacteria and 15B1 bacteria and collection of filtrate

[0013] On the PDA plate inoculated with sj18, punch 2-3 holes with a 1cm hole puncher to obtain 2-3 bacterial blocks, inoculate the bacterial blocks into a 1L shake flask containing 500mL PDB medium, shake at 200rpm and culture at 32°C 4-5 days to obtain the initial sj18 strain;

[0014] At the same time, pick 15B1 single clone on the PDA plate of 15B1 bacteria, inoculate in a 50mL tube containing 20mL PDB medium, shake at 200rpm and culture at 32°C for 48h to obtain the initial 15B1 strain;

[0015] Take a small amount of sj18 and 15B1 bacterial liquid for drying and quantification, and determine that the ratio of mixed fermentation bacterial liquid is roughly between 10-100 of sj18:15B1, and inoculate these two kinds of bacteria in a 50L stirred fermenter containing 25L culture liquid for fermentation . Fermentation conditions: The fermentation substrate is 10% malt, the st...

Embodiment 2

[0016] Example 2: Bacterial content analysis of different preparations of the mixed fermentation filtrate of sj18 bacteria and 15B1 bacteria

[0017] Concentrate 3L of the mixed bacterial liquid fermentation product of sj18 bacteria and 15B1 bacteria into 300mL by centrifugation, add 300g of maltodextrin as a protective agent, and use YAMATO ADL311 fog dryer for spray drying. The spray parameters are inlet temperature 115°C, outlet temperature 50°C degree, to obtain dry powder 13.7g. Dissolve 0.01g of dry powder in 1mL of sterile water, and then take 100μL of the solution and smear it on a 9cm medium plate. After calculation, the average number of bacteria on the plate is about 1500, so that the number of live bacteria in the spray-dried powder is roughly 1.5×10 6 pcs / g. The dry powder obtained after spray drying is in a state that is convenient for the preservation and transportation of active bacteria;

[0018] figure 1 It is a live bacteria detection plate for bacterial...

Embodiment 3

[0019] Embodiment 3: the effect of the mixed fermentation filtrate of sj18 bacterium and 15B1 bacterium spraying in the Hickory Carya Forest

[0020] Spray test method of 15B1 bacteria: Hangzhou Jiande Pecan Park, select variety 'Jinhua No. 1' ( Carya illinoinensis ) 12 individual plants are grouped and paired according to their yields last year, and those with similar yields are divided into the same group, which are represented by numbers -1 and -2, and two groups of spraying and non-spraying are respectively set. When spraying, dissolve 20 g of the dry powder of the mixed fermentation filtrate of sj18 bacteria and 15B1 bacteria in 20 L of water, and spray once on May 10, June 10, and July 10, respectively. The output of the statistical year is shown in Table 1, and it can be seen that the output of pecans with thin shells has increased significantly after spraying the bacterial fertilizer. Some hybrid combinations that were previously difficult to bear fruit also appear...

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Abstract

The invention relates to a bacterial fertilizer for carya illinoensis, which is characterized in that the bacterial fertilizer for carya illinoensis is composed of a fermentation broth obtained by mixed fermentation of a xylariaceae fungus (Xylariaceae fungus) sj18 strain and a pichia fermentans (Pichia fermentans) 15B1 strain or a dry powder obtained by concentrating and drying the fermentation broth. The method comprises the following steps: spraying 10 times of diluent of fermentation liquor for 1-3 times within three months from the beginning of pollen scattering of male flowers of the carya illinoensis to the fruiting of female flowers of the carya illinoensis, with the spraying amount of 1-2 liters for each tree, so that the method has the capability of remarkably maintaining and prolonging the pollen viability of the carya illinoensis, effectively improving the flowering asynchronism and increasing the pollination rate; meanwhile, black disease spots in the carya illinoensis fruit forming process are inhibited, and the phenomena that in China, the carya illinoensis forest land yield is low, flower and fruit falling is serious, and the fruit quality is poor due to the fruit black spot phenomenon are remarkably improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the fungi of the family Xyloceraaceae ( Xylaria radix ) sj18 strain and Pichia fermentum ( Pichia fermentans ) The application of the mixed ferment of 15B1 strain in the aspect of pecan fruiting. Background technique [0002] Thin-shelled hickory is one of the most economically valuable tree species in the Juglandaceae Hickory, native to the United States. In the place of origin, there are more than 1,000 varieties of pecans with thin shells. The fruits are large, the shells are thin, the kernel yield is high (50%-70%), the kernels are easy to get, and the yield is very high (1500-2250 kg / ha, 100 ~150 kg / mu). The nuts are delicious and fragrant, without astringency, rich in nutrition (72% fat, 11% protein, 13% carbohydrates), high in amino acids, and rich in vitamins B1 and B2. Kernel oil contains 73% monounsaturated fatty acid and has a calorific value of 32 kcal / kg. After eati...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/60C05G5/23A01C21/00
CPCC05F11/08C05G3/60C05G5/23A01C21/005
Inventor 彭华正朱汤军金群英
Owner ZHEJIANG FORESTRY ACAD
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