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Extraction method of bovine achilles tendon collagen

A technology of tendon collagen and extraction method, applied in peptide preparation methods, chemical instruments and methods, animal/human protein, etc., can solve problems such as difficulty in removal, protein variability, long enzymatic hydrolysis time, etc., to save production costs , good biological activity, non-degradable effect

Pending Publication Date: 2022-05-10
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is: in order to solve the low efficiency of collagen extraction and the unstable physical and chemical properties of the extracted collagen, protein variability, large amount of enzyme addition, long enzymolysis time, and large energy consumption; some methods use ethanol, etc. in the extraction process Organic solvents make it remain in the final product and are difficult to remove; the requirements for experimental equipment are high, the experimental operation is complicated, and it is easy to cause pollution to the environment to varying degrees, and a bovine Achilles tendon collagen is proposed. Extraction Method

Method used

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  • Extraction method of bovine achilles tendon collagen

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Effect test

Embodiment 1

[0043] The invention provides a technical solution: a method for extracting bovine Achilles tendon collagen, which is characterized in that it specifically includes the following steps:

[0044] S1. Pretreatment of bovine Achilles tendon: Soak and thaw the "Y"-shaped bovine Achilles tendon at room temperature, soak the bovine Achilles tendon in a 1L beaker containing 800mL of purified water, change the water every 5 minutes, continuously Change the water to clean the Achilles tendon 5 times, wash it with purified water, and soak it in a 4°C refrigerator for 24 hours; gently scrape it with a scalpel to remove the fascia on the surface of the bovine Achilles tendon. During the process of removing the fascia, it should be continuously wiped with gauze Until the surface of the bovine Achilles tendon has no fascia rolled up;

[0045] S2. Pre-treatment: After immersing the fascia-removed Achilles tendon in a refrigerator at 4°C for 24 hours, place the Achilles tendon in a refrigerator...

Embodiment 2

[0056] The invention provides a technical solution: a method for extracting bovine Achilles tendon collagen, which is characterized in that it specifically includes the following steps:

[0057] S1. Pretreatment of bovine Achilles tendon: Soak and thaw the "Y"-shaped bovine Achilles tendon at room temperature, soak the bovine Achilles tendon in a 1L beaker containing 800mL of purified water, change the water every 5 minutes, continuously Change the water to clean the Achilles tendon 5 times, wash it with purified water, and soak it in a 4°C refrigerator for 24 hours; gently scrape it with a scalpel to remove the fascia on the surface of the bovine Achilles tendon. During the process of removing the fascia, it should be continuously wiped with gauze Until the surface of the bovine Achilles tendon has no fascia rolled up;

[0058] S2. Pre-treatment: After immersing the fascia-removed Achilles tendon in a refrigerator at 4°C for 24 hours, place the Achilles tendon in a refrigerat...

Embodiment 3

[0069] The invention provides a technical solution: a method for extracting bovine Achilles tendon collagen, which is characterized in that it specifically includes the following steps:

[0070] S1. Pretreatment of bovine Achilles tendon: Soak and thaw the "Y"-shaped bovine Achilles tendon at room temperature, soak the bovine Achilles tendon in a 1L beaker containing 800mL of purified water, change the water every 5 minutes, continuously Change the water to clean the Achilles tendon 5 times, wash it with purified water, and soak it in a 4°C refrigerator for 24 hours; gently scrape it with a scalpel to remove the fascia on the surface of the bovine Achilles tendon. During the process of removing the fascia, it should be continuously wiped with gauze Until the surface of the bovine Achilles tendon has no fascia rolled up;

[0071] S2. Pre-treatment: After immersing the fascia-removed Achilles tendon in a refrigerator at 4°C for 24 hours, place the Achilles tendon in a refrigerat...

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Abstract

The invention discloses an extraction method of bovine tendo calcaneus collagen, and belongs to the technical field of biomedical materials, bovine tendo calcaneus pretreatment: soaking and unfreezing Y-shaped bovine tendo calcaneus at room temperature, cleaning the Y-shaped bovine tendo calcaneus with purified water, and soaking the cleaned Y-shaped bovine tendo calcaneus in a refrigerator at 4 DEG C for 24 hours; the method comprises the following steps: gently scraping and removing fascia on the surface of the bovine achilles tendon by using a scalpel, and performing pretreatment: continuing to soak the bovine achilles tendon without fascia in a refrigerator at 4 DEG C for 24 hours, freezing the bovine achilles tendon in a refrigerator at minus 20 DEG C, then slicing the frozen and hard bovine achilles tendon by using a slicing machine, weighing, and finally continuing to soak the bovine achilles tendon slices in the refrigerator at 4 DEG C for 48 hours. According to the method, the collagen is extracted mainly by combining acetic acid and pepsase, the collagen can only be degraded by collagenase, and other proteases cannot cut off collagen peptide chains, so that the pepsase can only cut off terminal peptides of the collagen and cannot cut off the peptide chains of the collagen; therefore, the finally extracted collagen can retain the complete triple helix structure.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a method for extracting bovine Achilles tendon collagen. Background technique [0002] Collagen is a kind of biological macromolecular substance with many types, and it is also an important part of extracellular matrix. The protein with the most content in the body accounts for about 25%-30% of the total protein in the body; at present, the commonly used collagen extraction methods at home and abroad mainly include hot water extraction, acid extraction, alkali extraction and enzymatic hydrolysis. The hot water extraction method has a low yield of collagen; the acid method is completely hydrolyzed, the tryptophan is easily completely destroyed, and the serine and tyrosine are also partially destroyed; the extraction of collagen under alkaline conditions is easy to cause protein denaturation; enzymatic hydrolysis method The mild conditions for extracting collagen can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/30C07K1/34C07K1/36
CPCC07K14/78C12P21/06
Inventor 丁鹏刘建强魏强马保华
Owner NORTHWEST A & F UNIV
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