In-situ-crossly-linked electrospun fibrous membrane dressing made from collangen I and preparation method of in-situ crosslinking electrospun fibrous membrane dressing

A technology of electrospinning fibers and in-situ cross-linking, applied in fiber processing, plant fibers, animal fibers, etc., can solve the problems of differences and misunderstandings between collagen and gelatin, and achieve the effect of good biocompatibility

Active Publication Date: 2015-07-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, we take the liberty to speculate that the "collagen" dissolved by this environment-friendly solvent is actually gelatin, and gelatin is easily soluble in water compared with collagen. Therefore, although the disclosed content of this invention is related to the environment-friendly solvent, However, there are still some misunderstandings in the preparation of collagen by electrospinning, and there is no distinction between collagen and gelatin in terms of structure, which is worthy of the direction of follow-up scientific research workers
Moreover, the traditional cross-linking methods and methods have not been broken through for the obtained microfiber membrane, and the spinning and cross-linking are still separated.

Method used

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  • In-situ-crossly-linked electrospun fibrous membrane dressing made from collangen I and preparation method of in-situ crosslinking electrospun fibrous membrane dressing
  • In-situ-crossly-linked electrospun fibrous membrane dressing made from collangen I and preparation method of in-situ crosslinking electrospun fibrous membrane dressing
  • In-situ-crossly-linked electrospun fibrous membrane dressing made from collangen I and preparation method of in-situ crosslinking electrospun fibrous membrane dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) To prepare the collagen electrospinning component A solution, first dissolve LiCl in [EMIM][Ac] with a mass fraction of 0.1%, and then use this solvent system to dissolve cowhide type I collagen, where the collagen mass fraction is 5% , during the whole dissolution process, keep the temperature at 4°C, and the dissolution time is 24h;

[0039] (2) To prepare the cross-linking component B liquid, firstly, the commercially available oxidized sodium alginate was dialyzed, the molecular weight cut-off of the dialysis bag was 3500 Daltons, the dialysate was deionized water, and the dialyzing time was 3 days, after which it was dissolved In deionized water, prepared as a solution with a mass fraction of 3%, the dissolution time was 48 hours, and left to stand for defoaming;

[0040] (3) Mix Component A and Component B evenly at a volume ratio of 4:6. After ultrasonic defoaming, place them in the same injection device in the electrospinning device for electrospinning. The ...

Embodiment 2

[0044] (1) To prepare collagen electrospinning component A, firstly dissolve LiAc in [EMIM][Ac] with a mass fraction of 0.5%, and then use this solvent system to dissolve pigskin type I collagen, where the mass fraction of collagen is 9 %, during the whole dissolution process, keep the temperature at 4°C, the dissolution time is 24h, and stand for defoaming;

[0045] (2) To prepare the cross-linking component B liquid, firstly, the commercially available oxidized sodium alginate was dialyzed, the molecular weight cut-off of the dialysis bag was 5000 Daltons, the dialysate was deionized water, and the dialyzing time was 4 days. In deionized water, prepared as a solution with a mass fraction of 6%, the dissolution time was 48 hours, and ultrasonic defoaming;

[0046] (3) Component A and component B are ultrasonically degassed at a volume ratio of 5:5 and placed in an independent injection device in an electrospinning device for electrospinning. The positive high voltage is 20kv,...

Embodiment 3

[0050] (1) To prepare collagen electrospinning component A liquid, first LiClO 4 Dissolve in [EMIM][Ac] with a mass fraction of 1%, and then use this solvent system to dissolve bovine Achilles tendon type I collagen, wherein the collagen mass fraction is 15%, and keep the temperature at 4°C during the entire dissolution process. Dissolving time is 48h, stand still for defoaming;

[0051] (2) To prepare the cross-linking component B liquid, first, dialyze the commercially available oxidized sodium alginate, the molecular weight cut-off of the dialysis bag is 8000 Daltons, the dialysate is deionized water, and the dialyzing time is 3 days, then dissolve it In deionized water, prepared as a solution with a mass fraction of 13%, the dissolution time was 48h, and ultrasonic defoaming;

[0052] (3) Component A and component B are ultrasonically degassed at a volume ratio of 6:4 and then placed in an independent injection device in the electrospinning device for electrospinning. The...

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Abstract

The invention discloses a preparation method for preparing in-situ cross-linked type I electrospun fiber membrane dressing, which uses a novel process-friendly solvent system (1-ethyl-3-methylimidazole acetate and lithium salt Composite system) dissolves type I collagen, retains the triple helix structure of type I collagen to the greatest extent, and uses a natural cross-linking agent (sodium alginate oxide) with good biocompatibility to participate in the electrospinning process of collagen, And cross-linking occurs in situ on type I collagen. Then, after simple water washing to remove the salt, freeze-drying, sterilization and packaging, the in-situ cross-linked type I collagen electrospun fiber membrane dressing is obtained. The method is simple and quick, easy to realize industrial production, and effectively avoids the risk of potential biological toxicity brought about by the currently commonly used glutaraldehyde crosslinking. The in-situ cross-linked type I collagen electrospun fiber membrane dressing prepared by the invention has good biocompatibility, no cytotoxicity and high bioactivity.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a method for preparing an in-situ crosslinked type I collagen electrospun fiber membrane dressing. Background technique [0002] Since the 20th century, nanotechnology and materials have gradually become household names. At present, nanomaterials have appeared more and more widely in people's actual work and life, bringing about rapid changes in people's lives. Electrospinning technology, as a method of rapid preparation of nanofiber materials, has attracted extensive attention of researchers. Especially in the field of biological dressings, the electrospun fibers are light, soft and full of nano-pores, which can fully heal the wound surface. On the one hand, it can reduce the external stimulation of the wound tissue, protect the wound body fluid from evaporating in the air, and at the same time ensure the energy of the wound surface. Exposure to fresh air is conducive to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32D04H1/4382D04H1/728D06M11/05D06M101/10D06M101/04
Inventor 胡杨但卫华但年华
Owner SICHUAN UNIV
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