Method for culturing cartilage cells and cartilage in vitro to obtain material for treating articular cartilage defect
A technology of chondrocytes and culture medium, which is applied in the field of obtaining materials and damaged tissues for the treatment of damaged tissues, and can solve the problems of unfavorable patients, limited effects, and high prices for treatment benefits and prices
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0094] Example 1 - Attempt to grow only fragments of hyaline cartilage tissue according to the invention
[0095] To create a homogeneous flap of cartilage tissue using an in vitro method to obtain material for the treatment of articular cartilage defects. First, clinical material was obtained from adults in the form of fragments of hyaline cartilage tissue collected from the minimal weight-bearing part of the knee joint. Collect tissue from locations where the patient's body load is low. This tissue should be healthy. Therefore, the shape of the collected flap or flaps of cartilage may vary. One or more cartilage fragments may be collected. One or several fragments can be divided into smaller fragments by cutting across or along the cartilage-bone line. Next, where a large piece of articular cartilage is harvested, it is cut into small pieces under sterile conditions.
[0096] Immediately after harvesting the material from the patient, the harvested material was transf...
Embodiment 2
[0110] Example 2 - Attempt to grow only fragments of cartilage tissue according to the invention
[0111] The cultivation process was similar to that described in Example 1.
[0112] Transfer compatible tissue patches to wells of a 6-well sterile cell culture plate filled with 2.5 ml of cell culture medium 2 supplemented with 1X concentration of Glutamax supplement and glucose [4.5 g / l].
[0113] After five months of culture, no connection was observed between pairs of cartilage tissue fragments placed in one well. There is no new tissue growth on the surface of old tissue.
[0114] Example 3 - Attempt to grow only fragments of cartilage tissue according to the invention
[0115] The culture procedure was performed as described in Example 1 except that compatible tissue sections were transferred to a 6-well sterile cell culture plate containing 2.5 ml of Cell Culture Medium 1 containing: anhydrous Calcium chloride, ferric nitrate, potassium nitrate chloride, magnesium sul...
Embodiment 4
[0117] Example 4 - General description of the method according to the invention
[0118] To create a homogeneous flap of cartilage tissue using an in vitro method to obtain material for the treatment of articular cartilage defects. First, clinical material in the form of fragments of hyaline cartilage was obtained from the knee joint of an adult. Hyaline cartilage is best harvested from the treated joint (could be knee, hip, ankle, shoulder or any other joint). When the treated joint is small or difficult to extract hyaline cartilage, hyaline cartilage can be harvested from another joint. It should always be the part that bears the least weight. Tissue from the patient is collected from a place of low body load. This tissue should be healthy. Therefore, the shape of the harvested flap or flaps of cartilage may vary. One or more cartilage fragments may be harvested. One or several fragments can be divided into smaller fragments by cutting across or along the cartilage-b...
PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
| thickness | aaaaa | aaaaa |
| thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More