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Application of bacillus coagulans BC99 in preparation of products for relieving colitis or immunomodulatory products

A technology of bacillus coagulans and immune regulation, applied in the field of microbiology, can solve the problems of unspecified strains in alleviating digestive tract diseases, unreported inhibition of bacillus coagulans BC99, etc.

Active Publication Date: 2022-05-13
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned prior art does not report the inhibitory effect of the Bacillus coagulans BC99 on other pathogenic bacteria, nor does it provide the use of this bacterial strain in alleviating digestive tract diseases

Method used

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  • Application of bacillus coagulans BC99 in preparation of products for relieving colitis or immunomodulatory products
  • Application of bacillus coagulans BC99 in preparation of products for relieving colitis or immunomodulatory products
  • Application of bacillus coagulans BC99 in preparation of products for relieving colitis or immunomodulatory products

Examples

Experimental program
Comparison scheme
Effect test

experiment example 3

[0054] Enterotoxigenic Escherichia coli (ETEC), enteropathogenic Escherichia coli (EPEC), Shigella flexneri and Listeria monocytogenes used in Experimental Example 3 were purchased from Guangdong Microbial Culture Collection Center.

[0055] The mice used in Experimental Examples 4-9 were purchased from the Animal Center of Huazhong Agricultural University.

[0056] Embodiments of the present invention provide Bacillus coagulans BC99 in inhibiting Shigella flexneri and / or utilizing Ellman's reagent or ketone propionate or ancient sugar and / or preparing products for relieving diarrhea or products for relieving colitis or products for immune regulation or regulation For use in intestinal flora preparations, the preservation number of the Bacillus coagulans BC99 is CGMCC No.21801.

[0057] Anyone who cultivates, multiplies, ferments, produces, prepares, or utilizes Bacillus coagulans BC99 with the preservation number CGMCC No. 21801 to inhibit Shigella flexneri or prepare product...

experiment example 1

[0068] Experimental Example 1, Physiological and biochemical experiments of Bacillus coagulans BC99

[0069] The physiological and biochemical characteristics of Bacillus coagulans strain BC99 were identified by automatic microbial analysis system and VITEK BCL identification card. The results of physiological and biochemical characteristics are as follows: the strain can utilize D-mannose, L-rhamnose, maltotriose, D-melezitose, D-glucose, D-galactose, N-acetyl-D-glucosamine, D-tagatose, D-ribose, ancient sugar, D-trehalose, α-galactosidase, phenylalanine arylaminease, alanine-phenylalanine-proline arylaminease, L -Proline aromatase, methyl-α-D-glucopyranidase, β-glucosidase, α-glucosidase, β-galactosidase, tyrosine arylase, L- Pyrrolidone arylamase. Pyruvate, Ellman's reagent, and escin hydrolysis were positive.

[0070] Bacillus coagulans BC99 can specifically use the following substances to carry out the following experiments:

[0071] (1) Ability to utilize carbohydrat...

experiment example 2

[0080] Experimental example 2, the tolerance of Bacillus coagulans BC99

[0081] (1) Preparation of simulated artificial gastric juice: prepare 0.5% sodium chloride solution, add 0.3% pepsin, adjust the pH value to 2.5 with 1mol / L HCL, then fully dissolve and filter with a 0.22μm microporous membrane for use.

[0082] (2) Bile salt culture medium: 0.3% bile salt, 0.2% sodium thioglycolate, MRS liquid medium, pH 6.0-6.2, sterilized at 118°C for 15 minutes.

[0083] (3) Tolerance test: prepare Bacillus coagulans BC99 bacterial suspension, take 1mL respectively, centrifuge and collect the bacteria, then add 1mL of the above-mentioned different treatment solutions, incubate at 37°C for 2.5h, and measure the treatment by dilution plate method The total number of viable bacteria (CFU / mL) before and after was calculated to calculate the survival rate. Among them, survival rate%=Nt / N0×100%, where N0 represents the number of viable bacteria (CFU / mL) of the strain without tolerance, an...

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Abstract

The invention discloses application of bacillus coagulans BC99 in preparation of products for relieving colitis or immunoregulation products, and belongs to the technical field of microorganisms. The invention provides application of bacillus coagulans BC99 with the preservation number of CGMCC No.21801 in inhibiting shigella flexneri and / or utilizing an Ellman reagent or ketone propionate or ancient sugar and / or preparing a product for relieving diarrhea or a product for relieving colitis or an immunoregulation product or a product for regulating intestinal flora. The bacillus coagulans BC99 is proved to have remarkable effects in the aspects of promoting the growth of beneficial microorganisms such as bifidobacteria, inhibiting the growth of harmful microorganisms such as shigella flexneri, improving the immunity of intestinal tracts, improving the expression level of IL-10, effectively relieving colitis, restoring the length of colon and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the use of bacillus coagulans BC99 in preparing products for relieving diarrhea / colitis or immune regulation products. Background technique [0002] Bacillus coagulans ( Bacillus coagulans ), Gram-positive, belonging to the Firm (or Firmicutes) phylum. Bacillus coagulans belongs to the genus Bacillus taxonomically, the cells are rod-shaped, Gram-positive bacteria, terminal spores, and no flagella. Decompose sugar to produce L-lactic acid, which is a homolactic acid fermenting bacteria. The optimum growth temperature is 45-50°C, and the optimum pH is 6.6-7.0. [0003] A strain of probiotic Bacillus coagulans BC99 recorded in patent application 202110285874.6 has been proven to have a strong ability to inhibit pathogenic bacteria, is sensitive to a variety of antibiotics, has high biological safety, and has potential probiotic functions. The bacterial powder ...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L2/39A23L2/52A23G3/36A23C9/16A23C9/152A23C9/12A61K35/742A61P31/04A61P1/00A61P37/02
CPCA23L33/135A23L2/39A23L2/52A23G3/366A23C9/16A23C9/152A23C9/1203A61K35/742A61P31/04A61P1/00A61P37/02A23V2002/00A23V2200/3204A23V2200/324Y02A50/30
Inventor 方曙光朱明明孔素芬刘欢项丽娟黄琴琴
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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