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Composite water adjuvant vaccine of porcine circovirus type 2 (PCV2) and preparation method of composite water adjuvant vaccine

A porcine circovirus and vaccine technology, applied in antiviral agents, biochemical equipment and methods, viruses, etc., can solve problems such as inability to effectively respond to disease infection, improve non-specific immunity, reduce mortality, and enhance immunity. original effect

Pending Publication Date: 2022-05-13
成都依思康生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Porcine circovirus type 2 (PCV2) vaccines are generally immunized in the nursery stage after weaning piglets. After immunization, traditional oil adjuvant vaccines, due to insufficient immune stimulation and interference of maternal antibodies in piglets, have a period of time after immunization. The level of internal antibodies shows that it first suppresses and then rises, and it has been in a low-level antibody period for quite a period of time, unable to effectively respond to disease infections

Method used

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  • Composite water adjuvant vaccine of porcine circovirus type 2 (PCV2) and preparation method of composite water adjuvant vaccine
  • Composite water adjuvant vaccine of porcine circovirus type 2 (PCV2) and preparation method of composite water adjuvant vaccine
  • Composite water adjuvant vaccine of porcine circovirus type 2 (PCV2) and preparation method of composite water adjuvant vaccine

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Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1, determination and preparation of porcine circovirus type 2 (PCV2) compound water adjuvant vaccine preparation method

[0036] 1. Porcine circovirus type 2 (PCV2) compound water adjuvant vaccine formulation determined

[0037] Select turmeric saponin, panax notoginseng saponins, and Lycium barbarum polysaccharide as the main components of the water adjuvant, and use KollisolvPEG E 400 (US Sigma Company, article number: 06855, CAS number: 25322-68-3) as the nonionic surfactant to stabilize the water Mutually.

[0038] Orthogonal experiment was designed, three concentrations of turmeric saponin, panax notoginseng saponins, and Lycium barbarum polysaccharide were set respectively, and three groups of ratios were set for the proportion of PEG E 400 and saline, and mixed solutions were respectively configured according to the principle of orthogonal experiment. It conducts physical properties and stability index testing, and screens out the vaccine compound wat...

Embodiment 2

[0046] Embodiment 2, porcine circovirus type 2 (PCV2) composite water adjuvant vaccine quality evaluation

[0047] 1. Test the stability of the compound water adjuvant vaccine after centrifugation

[0048] After being centrifuged at 10000r / min for 30 minutes, it still maintained a uniform, transparent, and clear appearance. There was no material precipitation and stratification. Compared with before centrifugation, the color, clarity, and fluidity did not change. The porcine circovirus type 2 ( PCV2) complex water adjuvant vaccine has good stability.

[0049] 2. Determination of pH of compound water adjuvant and compound water adjuvant vaccine

[0050] Each take a certain amount of compound water adjuvant and compound water adjuvant vaccine into a clean beaker, the pH meter used is Mettler-Toledo (FE28), measure three times, take the average value, the compound water adjuvant and compound water adjuvant vaccine pH were 5.8 and 6.5, within the allowable range of vaccine pH.

...

Embodiment 3

[0053] Embodiment 3, porcine circovirus type 2 (PCV2) composite water adjuvant vaccine safety test

[0054] 1. Safety experiment of compound water adjuvant vaccine on mice

[0055] Twenty 6-week-old BALB / c mice with a body weight of 18-22 grams were randomly divided into 2 groups of 10 mice each, and injected subcutaneously with 500 μl of compound water adjuvant vaccine and 500 μl of normal saline respectively, and the health performance and survival of the mice were observed daily The situation was continuously observed for 7 days, and there were no adverse reactions or deaths caused by vaccine injection.

[0056] 2. Safety experiment of compound water adjuvant vaccine on piglets

[0057] 10 healthy piglets aged 28-35 days, no porcine circovirus type 2 (PCV2) virus and antibodies were detected by PCR and ELisa, were randomly divided into 2 groups of 5 piglets, and the body temperature data of 2 days before injection were detected and recorded. 2 times a day; the two groups ...

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Abstract

The invention relates to a composite water adjuvant vaccine of porcine circovirus type 2 (PCV2) and a preparation method of the composite water adjuvant vaccine. Specifically, the vaccine comprises: (1) a composite water adjuvant; (2) a porcine circovirus type 2 (PCV2) antigen; the compound water adjuvant is prepared from the following components in percentage by weight: 1 to 10 mg / ml of diosgenin, 1 to 10 mg / ml of panax notoginseng saponins, 1 to 10 mg / ml of lycium barbarum polysaccharide, 0.5 to 2 percent of PEG (Polyethylene Glycol) E 400, 0.5 to 2 percent of vitamin C, 0.5 to 2 percent of vitamin C and the balance of water. And the balance of water.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a composite water adjuvant vaccine of porcine circovirus type 2 (PCV2) and a preparation method thereof. Background technique [0002] Porcine circovirus disease is caused by porcine circovirus type 2 (PCV2). There is a wide range of porcine infectious diseases in my country's pig industry. Among them, weaned piglets are susceptible pigs, and the clinical symptoms are mostly multi-system in weaned piglets. The wasting syndrome has brought considerable economic losses to the pig industry in my country. [0003] A vaccine adjuvant is a substance that is used together with an antigen and has no immunogenicity itself, but can stimulate the body's non-specific and specific immunity, allowing the immune system to quickly recognize and present the antigen. At present, veterinary vaccine adjuvants are mainly divided into water-in-oil adjuvants, oil-in-water adjuvants, water-in-oi...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61K39/39C07K14/01A61P31/20
CPCA61K39/12A61K39/39C07K14/005A61P31/20A61K2039/55577A61K2039/55583A61K2039/55555A61K2039/575A61K2039/572C12N2750/10022C12N2750/10034
Inventor 杨豫蒙邹智坤陈强李菁侯钰
Owner 成都依思康生物科技有限公司
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