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Application of umbilical artery in preparation of graft blood vessel material for coronary artery bypass grafting and method for preparing graft blood vessel material for coronary artery bypass grafting

A technology of coronary artery bypass and umbilical artery, applied in the field of biomedicine, to prevent spasm, less impact on physical properties of blood vessels, and long-term preservation

Inactive Publication Date: 2022-05-17
李平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the clinical application research of umbilical artery is still in the exploratory stage.

Method used

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  • Application of umbilical artery in preparation of graft blood vessel material for coronary artery bypass grafting and method for preparing graft blood vessel material for coronary artery bypass grafting
  • Application of umbilical artery in preparation of graft blood vessel material for coronary artery bypass grafting and method for preparing graft blood vessel material for coronary artery bypass grafting
  • Application of umbilical artery in preparation of graft blood vessel material for coronary artery bypass grafting and method for preparing graft blood vessel material for coronary artery bypass grafting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Physiological and anatomical research of embodiment 1 umbilical artery

[0079] 1. Collection and preparation of umbilical artery materials. The collection of umbilical arteries was completed within 4-6 hours after fetal delivery, and all operations were performed under sterile conditions. Such as figure 1 shown.

[0080] Obtain blood vessels from the umbilical cord strictly following non-invasive techniques; after obtaining the umbilical cord, place it in sterile saline containing papaverine and 1‰ heparin, and use it to flush the vessel lumen to prevent vasospasm and thrombosis; keep the prepared solution at room temperature for 37 ℃; perfusion pressure <200mmHg. After fully freeing the umbilical artery, place it in normal saline with papaverine (30mg / 100ml) and 1‰ heparin.

[0081] 2. Detection of the physical properties of the umbilical artery--the compliance and elasticity of the umbilical artery and the pressure test.

[0082] Experimental method: Measure the...

Embodiment 2

[0121] Example 2 Antigen removal, anti-calcification treatment and related performance detection of umbilical artery after treatment

[0122] Purpose:

[0123] It can be known from Example 1 that the umbilical artery of newborns has the potential to be used as a material for coronary artery bypass grafting. However, if the neonatal umbilical artery is used clinically, it is an allogeneic blood vessel and has antigenicity. Therefore, it must be treated with anti-antigen and anti-calcification before using the umbilical artery. In this experiment, we will use the anti-antigenic and anti-calcification methods we have explored to treat the umbilical artery, and then test the effect of this method and the impact on the related properties of the umbilical artery, hoping to find a clear effect, little impact on the physical properties of the blood vessel, and safe , Simple and easy method, suitable for possible clinical use in the future.

[0124] Materials and Methods

[0125] 1....

Embodiment 3

[0213] The animal experiment research of embodiment 3 umbilical artery application

[0214] Purpose:

[0215] It can be known from Example 2 that after cryopreservation in liquid nitrogen + glutaraldehyde treatment of the umbilical artery, the anti-antigen and anti-calcification effects are good, and the physical properties of the blood vessels are less affected, and it is easy to implement, so it becomes our alternative for animal experiments. Vascular material. In order to further explore the applicability of the umbilical artery, we designed an animal model to conduct an in vivo study to observe the vascular patency and vascular morphological changes of the umbilical artery treated with cryogenic liquid nitrogen + glutaraldehyde, which provides a basis for the clinical application of the umbilical artery. Basic experimental data.

[0216] Materials and Methods

[0217] 1. Main materials and instruments:

[0218]

[0219] 2. Method:

[0220] (1) Rabbit carotid artery...

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Abstract

The invention relates to the field of biomedicine, in particular to application of an umbilical artery in preparation of a vascular graft material for coronary artery bypass grafting and a method for preparing the vascular graft material for coronary artery bypass grafting. The feasibility of applying the umbilical artery to coronary artery bypass grafting is discussed from the aspects of the physiological anatomical structure of the umbilical artery, the antigenic removal and anti-calcification treatment effect, animal experiments and the like, and a new vascular transplantation material source is found for coronary artery vascular reconstruction. Test results show that the umbilical artery can meet the requirements of being used as a blood vessel transplantation material for coronary artery bypass grafting in the aspects of physiological anatomical structure and physical performance; the diameter of the umbilical artery can be increased through hydraulic expansion, spasm is prevented, and obvious damage to the vascular structure is not caused. After the umbilical artery is treated by adopting a liquid nitrogen profound hypothermia and glutaraldehyde (GA) method, the antigenic and anti-calcification effects are good, the influence on the physical properties of blood vessels is relatively small, and the method is easy to implement and can be stored for a relatively long time.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of the umbilical artery in the preparation of grafted blood vessel materials for coronary artery bypass grafting and the method for preparing the grafted blood vessel materials for coronary artery bypass grafting. Background technique [0002] Since the 1990s, coronary artery bypass surgery has emerged in China. In the vascular grafts of coronary artery surgery, the grafting vessel replacement materials are usually autologous arteries such as internal mammary artery, radial artery, gastroepiploic artery, etc., or commonly used veins such as great saphenous vein, upper arm vein (basic vein), etc. ; however, the availability of native arteries or veins is often limited. In addition, the 15-year patency rate of autologous great saphenous vein graft is about 60%, and the 5-year patency rate of radial artery graft is 84%. Moreover, the graft vessels are affected by age, acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3625A61L27/507A61L27/3687A61L2430/40
Inventor 李平李玉琳尤斌李光叶钊飞
Owner 李平
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