Colloidal gold test strip for rapidly detecting hyaluronic acid

Abstract

The invention relates to the technical field of immunochromatography detection, in particular to a colloidal gold test strip for rapidly detecting hyaluronic acid. Protein with the sequence shown in SEQ ID NO: 1 is used as a detection antigen, the kit specifically comprises a PVC (polyvinyl chloride) rubber plate, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad are sequentially connected onto the PVC rubber plate, a detection line and a quality control line are arranged on the nitrocellulose membrane, the gold-labeled pad contains hyaluronic acid specific binding protein which is labeled by colloidal gold particles and is provided with a GST (glutathione S-transferase) label, and the quality control line is provided with a quality control line. Hyaluronic acid is anchored in the detection line, and a GST monoclonal antibody is anchored in the quality control line and can be specifically combined with a protein with a GST tag. The colloidal gold test strip provided by the invention is convenient and rapid to use, and can complete a chromatographic reaction within 10 minutes to realize rapid detection of hyaluronic acid in a sample.

Description

technical field [0001] The invention relates to the technical field of immunochromatographic detection, in particular to a colloidal gold test strip for rapid detection of hyaluronic acid. Background technique [0002] Hyaluronic acid (HA) is produced by fermentation of Streptococcus equisubsp.zooepidemicus using glucose, yeast powder, peptone, etc. Glycosaminoglycan composed of N-acetyl-D-glucosamine disaccharide units is a linear macromolecular polysaccharide. Since the hyaluronic acid molecule is in the form of a rigid helical column in its spatial structure, the hydroxyl groups on the inside of the column are highly hydrophilic. At the same time, the continuous and directional arrangement of the hydroxyl groups forms a highly hydrophobic area on the hyaluronic acid molecular chain, even at high concentrations. When it is very low, hyaluronic acid can also form a three-dimensional network structure, so hyaluronic acid has strong hygroscopicity, water retention and viscoe...

AI Technical Summary

Problems solved by technology

Colloidal gold test strips are widely used in the field of on-site rapid detection because of their short detection cycle, easy storage, easy operation, and simple and clear results. At present, there are no products that can be used for rapid detection of HA on the market. It is necessary to develop a colloidal gold test strip suitable for HA in cosmetics or food to fill the gap, so as to better guide enterprises in production quality control and law enforcement supervision

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