Probiotic fermentation product capable of dispelling melancholy and calming heart as well as preparation method and application of probiotic fermentation product
The technology of probiotics and fermented product is applied in the field of probiotics fermented product for relieving depression and calming heart and its preparation field, which can solve the problems of insufficient taking effect, low bioavailability, large liver damage and the like, so as to improve bioavailability and relieve immunity. Stress, the effect of regulating immune function
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Embodiment 1
1. Preparation of culture medium
1) Basal medium
6.0g yeast hydrolyzate, 2.0g corn steep liquor, 3.0g peanut flour, 10.0g molasses, 3.0g KCl, 0.1g ZnSO 4 ·8H 2 O, 2g FeSO 4 ·4H 2 O, 8.0g CaCO 3 Dissolve in 1L of purified water, adjust pH to 5.8, and prepare basal medium (when preparing solid medium, add 15g of agar).
[0063] 2) Fermentation medium
Put the fruit Chinese medicine composition into a CO 2 The immersion temperature is 20 ℃, the time is 3d, and then crushed and squeezed to obtain the raw material juice base; the purified water is added to the raw juice base to adjust the size, and the volume of the raw juice base and purified water is controlled. The ratio is 1:4, adding S0 2 80ml / L and 0.5% baking soda to prepare the raw material juice of fruit traditional Chinese medicine composition.
[0064] Add 50g molasses, 6g yeast hydrolyzate, 2g ferric sulfate and 8.0g CaCO to 1L of the above raw material juice 3 , adjust the pH value to 5.8 to obtain the ferme...
Embodiment 2
1. Preparation of culture medium
1) Basal medium
10.0g yeast hydrolyzate, 3.0g corn steep liquor, 4.0g peanut flour, 10.0g molasses, 2.0g KCl, 0.3g ZnSO 4 ·8H 2 O, 2.2g FeSO 4 ·4H 2 O, 10.0g CaCO 3 Dissolve in 1 L of purified water, adjust the pH to 6.0, and prepare a basal medium (when preparing a solid medium, add 15 g of agar).
[0074] 2) Fermentation medium
Put the fruit Chinese medicine composition into a CO 2 The immersion temperature is 22 ℃, the time is 4d, and then crushed and squeezed to obtain the raw material juice base material; purified water is added to the raw material juice base material for slurrying, and the volume of the raw material juice base material and purified water is controlled. The ratio is 1:3, adding S0 2 80ml / L and 0.5% baking soda to prepare the raw material juice of fruit traditional Chinese medicine composition.
[0075] Add 60g molasses, 10g yeast hydrolyzate, 2.2g ferric sulfate and 10.0g CaCO to 1L of the above raw material ju...
Embodiment 3
1. Preparation of culture medium
1) Basal medium
10.0g yeast hydrolyzate, 3.0g corn steep liquor, 4.0g peanut flour, 10.0g molasses, 2.0g KCl, 0.3g ZnSO 4 ·8H 2 O, 2.2g FeSO 4 ·4H 2 O, 10.0g CaCO 3 Dissolve in 1 L of purified water, adjust the pH value to 6.0, and prepare a basal medium (when preparing a solid medium, add 15 g of agar).
[0085] 2) Fermentation medium
Put the fruit Chinese medicine composition into a CO 2 The immersion temperature is 24 ℃, the time is 5d, and then crushed and squeezed to obtain the raw juice base material; purified water is added to the raw juice base material for slurrying, and the volume of the raw juice base material and purified water is controlled. The ratio is 1:2, adding S0 2 80ml / L and 0.5% baking soda to prepare the raw material juice of fruit traditional Chinese medicine composition.
[0086] Add 60g molasses, 10g yeast hydrolyzate, 2.2g ferric sulfate and 10.0g CaCO to 1L of the above raw material juice 3 , adjust the p...
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