Method for detecting diversity influence of pinkflower clover on constructed wetland substrate microorganisms and application of method
A technology of artificial wetlands and detection methods, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, biostatistics, etc., and can solve problems such as high construction costs, small hydraulic loads, and weak processing capabilities
Pending Publication Date: 2022-06-03
WENZHOU MEDICAL UNIV
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Problems solved by technology
Constructed wetlands with surface flow have the disadvantages of large area, small hydraulic load, weak treatment capacity, and easy breeding of mosquitoes in summer.
The water purification ability of the vertical subsurface flow constructed wetland is the strongest among the three types of constructed wetlands, but the hydraulic flow of the sewage is short, the denitrification effect is weak, and the construction cost is high and the operation management is complicated
Method used
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Embodiment 1
[0033] 1 Materials and methods
[0034] 1.1 Sample Preparation and Collection
[0035] Artificial horizontal subsurface wetlands are constructed of PVC boards and filled with gravel (diameter 4-6mm) to form a filler matrix, the size of which is as follows figure 1 shown. A perforated tube is installed on the side of the device to facilitate sampling, and the collected sample is a gravel surface matrix. The planting density of windmill grass is about 25 plants per square meter, and the water flow direction is from left to right. Four areas 1, 3, 7, and 9 were selected as the sampling sites for this experiment. At the same time, groups were formed according to whether the windmill grass was planted or not. The unplanted windmill grass group was named group B: B1, B3, B7, and B9. The group of planting windmill grass was named as C group: C1, C3, C7, C9.
[0036] 1.2 Detection process
[0037] Take 1 g of sample and use a DNA extraction kit (OMEGA Soil DNA Kit) according t...
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Abstract
The invention provides a method for detecting the diversity influence of pinkflower grass on constructed wetland matrix microorganisms and application. The method comprises the following steps: (1) preparing and collecting a sample; (2) extracting DNA of a sample, detecting the concentration and purity of the DNA, and then detecting the integrity of the DNA sample; (3) carrying out PCR (Polymerase Chain Reaction) amplification; (4) performing high-throughput sequencing; (5) performing quality control, filtering, splicing and chimera removal; performing taxonomic analysis on the OTU representative sequence, and calculating alpha diversity according to the OTU data to obtain a correlation index; according to the invention, by comparing the community composition difference of a gravel surface matrix biological membrane under the condition of planting or not planting pinkgrass, amplifying and sequencing the bacterium 16S rDNA gene by using a high-throughput sequencing technology, and comparing the diversity of microorganisms in different constructed wetland environments, the flora structure and dominant bacteria of the microorganisms can be researched, and the application of the bacteria 16S rDNA gene to the artificial wetland environment can be realized. And help is provided for finally designing a more scientific constructed wetland scheme.
Description
technical field [0001] The invention relates to a method for detecting the influence of plants on the microbes of a constructed wetland matrix, in particular to a method and application for detecting the influence of the windmill grass on the diversity of the microbes of the constructed wetland matrix. Background technique [0002] Wetlands are a transitional zone, between terrestrial and aquatic ecosystems. Constructed wetlands, on the other hand, refer to an engineered wetland system that can be adjusted by man-made means. It is widely used in the field of water purification because of its ecological adjustment, reliable effect, and environmental friendliness. [0003] Constructed wetlands are classified into surface flow constructed wetlands, vertical subsurface flow constructed wetlands and horizontal subsurface flow constructed wetlands according to different hydraulic modes in operation. Surface flow constructed wetlands have disadvantages such as large area, small hy...
Claims
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IPC IPC(8): C12Q1/6869C12Q1/689G16B30/00G16B40/00G16B50/00
CPCC12Q1/6869C12Q1/689G16B30/00G16B40/00G16B50/00C12Q2531/113C12Q2535/122Y02W10/10
Inventor 葛利云邓欢欢周俊威蔡贤雷
Owner WENZHOU MEDICAL UNIV
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