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Method for separating eclipta saponin I from ovateleaf holly bark

A technology of Eclipta saponin and Jiubingying, which is applied in the field of separation of Eclipta saponin I, can solve the problem of complex separation methods of compounds, no separation of Eclipta saponin I, antibacterial mechanism and antibacterial components are not fully disclosed And other issues

Active Publication Date: 2022-06-21
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the complex chemical composition of Gyubing, the separation methods of each compound are also relatively complicated, and there are still many unisolated and identified compound components. The antibacterial mechanism and antibacterial components of Gyubing have not been fully disclosed, and no There are related reports on the isolation of Eclipta saponin I from Jiubing

Method used

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  • Method for separating eclipta saponin I from ovateleaf holly bark

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A method for separating Eclipta saponin I from Salvage Bing, comprising the following steps:

[0036] S1: Soak Savior in 75% ethanol solution, extract at room temperature for 2 days, filter to obtain filter residue, then soak in 45% ethanol solution, extract at room temperature for 2 days, filter again to obtain filter residue, soak in purified water, and extract at room temperature for 2 days , combined three filtrates, concentrated to obtain extract;

[0037] S2: the extract is extracted with petroleum ether, ethyl acetate, n-butanol and water successively, and each extraction layer is concentrated and dried;

[0038] S3: Dissolve the extract of the water extraction layer in water, then load the sample on the macroporous adsorption resin, and carry out gradient elution with 0-90% methanol solution, 30% is a gradient to obtain four fractions;

[0039] S4: Dissolve the fraction obtained by elution of 60% methanol solution in the above gradient elution in water, then lo...

Embodiment 2

[0042] A method for separating Eclipta saponin I from Salvage Bing, comprising the following steps:

[0043] S1: Soak Savior in 80% ethanol solution, extract at room temperature for 3 days, filter to obtain filter residue, then soak in 50% ethanol solution, extract at room temperature for 3 days, filter again to obtain filter residue, soak in purified water, and extract at room temperature for 3 days , combined three filtrates, concentrated to obtain extract;

[0044] S2-S5 are the same as Example 1.

Embodiment 3

[0046] A method for separating Eclipta saponin I from Salvage Bing, comprising the following steps:

[0047] S1: Soak Savior in 85% ethanol solution, extract at room temperature for 4 days, filter to obtain filter residue, then soak in 55% ethanol solution, extract at room temperature for 4 days, filter again to obtain filter residue, soak in purified water, and extract at room temperature for 4 days , combined three filtrates, concentrated to obtain extract;

[0048] S2-S5 are the same as Example 1.

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Abstract

The invention discloses a method for separating eclipta saponin I from ovateleaf holly bark, which comprises the following steps: sequentially soaking the ovateleaf holly bark with ethanol and water, merging filtrate, and concentrating to obtain extract; extracting the extract, concentrating and drying; dissolving the extract, loading a sample to macroporous adsorption resin, and carrying out gradient elution to obtain four fractions; a target fraction in the four fractions is sequentially loaded on a chromatographic column I, a chromatographic column II, a chromatographic column III and a chromatographic column IV for gradient elution, the obtained samples are dripped on thin-layer plates respectively, the fraction I, the fraction II, the fraction III and the fraction IV are collected according to the specific shift value, the fraction IV is prepared through a high performance liquid chromatograph, and the fraction V is collected to obtain the eclipta saponin I. According to the invention, the eclipta saponin I can be separated from the ovateleaf holly bark, a reference basis is provided for the material basic research of the ovateleaf holly bark and the antibacterial mechanism and clinical application research of the antibacterial part, and a new path is opened up for the preparation source of the eclipta saponin I.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of Chinese herbal medicines, and in particular relates to a method for separating Eclipta saponin I from Salvage. Background technique [0002] Salvage is the dried bark of Ilex rotunda Thunb, a plant of the holly family, which is mainly produced in Guangdong and Guangxi. , obtained after drying. It is cold in nature and bitter in taste, and belongs to the lung, stomach, large intestine, and liver meridians. It has the functions of clearing away heat and detoxifying, removing dampness and relieving pain. It is mainly used for colds and fever, tonsillitis, sore throat, acute and chronic pharyngitis, gastric and duodenal ulcers, rheumatism and joint pain, bruises, water and fire burns. [0003] With the continuous deepening of research on the traditional Chinese medicine Jiubing in recent years, its chemical components have been continuously separated, which can be roughly divide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61P31/04
CPCC07J63/008A61P31/04Y02A50/30
Inventor 司红彬钟昕柴贝贝廖荣游纯波严明王莉贞
Owner GUANGXI UNIV