Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of bifidobacterium bifidum B11 in preparation of product for inhibiting helicobacter pylori and repairing gastric mucosal barrier

A technology of Bifidobacterium bifidum and Helicobacter pylori, applied in the field of microbiology, can solve problems such as the increase of drug-resistant strains, adverse effects on the gastrointestinal micro-ecosystem, and poor inhibition of Helicobacter pylori

Active Publication Date: 2022-06-24
河北一然生物科技股份有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, when antibiotics are used to treat Helicobacter pylori infection, irregular abuse of antibiotics will have adverse effects on the gastrointestinal micro-ecological system, destroying the micro-ecological balance and microbial barrier of the gastrointestinal tract, and gradually reducing the antibiotic-sensitive strains in the intestinal tract , the increase of drug-resistant strains, and antibiotics can also cause many adverse reactions in some patients, such as abdominal pain, nausea, vomiting, diarrhea, etc., and the incidence tends to increase
Studies have shown that probiotics have the effect of inhibiting Helicobacter pylori, but the probiotics currently on the market have a poor inhibitory effect on Helicobacter pylori. Probiotics are necessary for clinical treatment of Helicobacter infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bifidobacterium bifidum B11 in preparation of product for inhibiting helicobacter pylori and repairing gastric mucosal barrier
  • Application of bifidobacterium bifidum B11 in preparation of product for inhibiting helicobacter pylori and repairing gastric mucosal barrier
  • Application of bifidobacterium bifidum B11 in preparation of product for inhibiting helicobacter pylori and repairing gastric mucosal barrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This example provides the inhibitory effect of Bifidobacterium bifidum B11 on Helicobacter pylori.

[0028] Bifidobacterium bifidum B11 was activated for 3 generations, inoculated in the modified MRS medium, and after anaerobic cultivation at 37 °C for 18 h, the density of the bacterial solution was adjusted to 1.0 × 10 8 About CFU / mL, centrifuge at 4°C and 5000r / min for 5min, collect the culture supernatant, filter and sterilize with a 0.22μm filter, and set aside.

[0029] The antibacterial test was carried out by the Oxford cup method, the solid medium was poured into the plate, and after solidification, the activated three-generation Helicobacter pylori stably passaged was suspended with sterile PBS, and the bacterial concentration was about (1×10 8 ), add 1ml to the plate, shake well, let stand for 3 minutes, and dry. Put the Oxford cup on the plate, add 100 μL of the above-filtered Bifidobacterium bifidum B11 culture supernatant to the well, cultivate it at 37°C ...

Embodiment 2

[0032] This example provides the adhesion effect of Bifidobacterium bifidum B11 on gastric cancer cells AGS.

[0033] The adherent gastric cancer cells AGS stably cultured for three consecutive generations were digested with trypsin to terminate the reaction. After centrifugation and washing, they were suspended with non-resistant F12 (10% fetal bovine serum) medium, and the concentration of the cell fluid was adjusted to 5×10 4 pcs / mL), were treated with climbing slices, and left to stand overnight for culture. After the cells were covered with glass slides, the slides were washed 3 times with PBS buffer; Bifidobacterium bifidum B11 was activated for 3 generations, inoculated in the modified MRS medium, and after anaerobic culture at 37 °C for 18 h, the density of the bacterial solution was adjusted to 1.0×10 8 About CFU / mL, centrifuge at 4°C and 5000r / min to collect bacterial cells, and adjust the concentration of bacterial liquid to 1×10 with non-resistant F12 (10% fetal b...

Embodiment 3

[0036] This example provides the application of Bifidobacterium bifidum B11 in inhibiting the adhesion of Helicobacter pylori to gastric cancer cells.

[0037] The adherent gastric cancer cells AGS stably cultured for three consecutive generations were digested with trypsin to terminate the reaction, and after centrifugation and washing, they were suspended with non-resistant F12 (10% fetal bovine serum) medium, and the concentration of the cell fluid was adjusted to 5 × 10 4cells / mL), 100 μL per well was inoculated into a 96-well plate, and cultured overnight at 37°C. After the cells covered the bottom of the well plate, washed three times with PBS buffer, and set up the following different groups to co-incubate with gastric cancer cell AGS.

[0038] Control group: Three generations of activated Helicobacter pylori were used to adjust the concentration of bacterial liquid to 1×10 with non-resistant F12 (10% fetal bovine serum) medium. 8 1 / mL, add 100 μL and then add 100 μL m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microorganisms, in particular to application of bifidobacterium bifidum B11 with the preservation number of CGMCC No.24381 in preparation of products for inhibiting helicobacter pylori and repairing gastric mucosal barriers. The bifidobacterium bifidum B11 provided by the invention has a remarkable inhibition effect on helicobacter pylori, has a strong adhesion effect with gastric cancer cells AGS, provides possibility for the bifidobacterium bifidum B11 to be adhered to gastric epithelial cells and peripheral mucous layers thereof, and has a potential of colonization in the stomach; the bifidobacterium bifidum B11 can obviously inhibit the adhesion of helicobacter pylori on gastric epithelial cells, and meanwhile, the bifidobacterium bifidum B11 can inhibit the transcriptional level of a proinflammatory factor IL-8, inhibit inflammation caused by the helicobacter pylori and play a role in protecting gastric mucosa. Meanwhile, the live strain of the strain can also promote transcription of mucous membrane protection factors TFF1, iNOS and COX-2, and has a positive effect on gastric mucosal barrier and cell repair.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of Bifidobacterium bifidum B11 in the preparation of products for inhibiting Helicobacter pylori and repairing gastric mucosal barrier. Background technique [0002] Helicobacter pylori, also known as Helicobacter pylori, Latin name Helicobacterpylori, referred to as Hp, is a Gram-negative, microaerophilic bacterium that lives in various regions of the stomach and duodenum. It can cause mild chronic inflammation of the gastric mucosa, or even lead to gastric and duodenal ulcers and gastric cancer. It has been identified as a class I carcinogen by the World Health Organization. Therefore, research on the prevention and treatment of Helicobacter pylori has great application value. . [0003] With the increase of strain variation, secondary drug resistance, cross drug resistance, and cross infection of different strains, the eradication rate of Helicobacter p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/745C12N1/20A23L33/135A61P1/04A61P31/04A61P35/00C12R1/01
CPCA61K35/745C12N1/20A23L33/135A61P1/04A61P31/04A61P35/00A23V2002/00A23V2400/517A23V2200/32
Inventor 赵林森杨玲路江浩高景伟孙策齐世华樊中利
Owner 河北一然生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com