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Thin film transistor for ultralow-limit detection of C-reactive protein and preparation method thereof

A technology of thin film transistors and reactive proteins, which is applied in the field of biosensors, can solve the problems of high detection limit of C-reactive protein, and achieve the effects of preventing leakage, low cost, and saving preparation time

Active Publication Date: 2022-06-28
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Solve the problem of high detection limit of C-reactive protein

Method used

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  • Thin film transistor for ultralow-limit detection of C-reactive protein and preparation method thereof
  • Thin film transistor for ultralow-limit detection of C-reactive protein and preparation method thereof
  • Thin film transistor for ultralow-limit detection of C-reactive protein and preparation method thereof

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Effect test

Embodiment 1

[0034] A preparation method of a thin film transistor for ultra-low limit detection of C-reactive protein, specifically comprising the following steps:

[0035] 1) Substrate treatment: The PET / ITO film is fixed on the glass sheet, and the oxygen plasma hydrophilic treatment is performed for 1 min; wherein, the glass sheet is the substrate 1, and the PET / ITO film is the gate 2.

[0036] 2) Active layer material: drop 500 μl of a PVA aqueous solution with a concentration of 15 mg / ml on the gate electrode 2 , and naturally cure for 12 hours to form the PVA insulating layer 3 . After annealing in a 60° C. oven for 2 hours, drop-coating 500 μl of a PMMA anisole solution with a concentration of 5 mg / ml, and annealing in a 90° C. oven for 30 minutes to form a PMMA insulating layer 4 .

[0037] 3) Spin-coating of active layer: spin-coat 8mg / ml P3HT-dichlorobenzene solution on PVA / PMMA at 2000r / min, and anneal on a hot plate at 120°C for 10min to form active layer 5 .

[0038] 4) Sour...

Embodiment 2

[0043] The sensors prepared in Example 1 were placed in 5pM, 50pM, 5×10 2 pM, 5×10 3 pM, 5×10 4 pM, 5×10 5 pM, 5×10 6The transfer curve obtained in pM CRP solution is as Image 6 shown. In the figure it can be seen that there is still a significant current change at CRP concentrations as low as 5 pM.

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Abstract

The invention belongs to the technical field of biosensors, and relates to a thin film transistor for ultralow-limit detection of C-reactive protein and a preparation method thereof. Comprising a substrate, a grid electrode, an insulating layer, a P3HT active layer, a source electrode and a drain electrode, wherein the source electrode and the drain electrode are arranged on the active layer; the insulating layer comprises a PVA (Polyvinyl Alcohol) insulating layer arranged on the grid electrode and a PMMA (Polymethyl Methacrylate) insulating layer arranged on the PVA insulating layer; an anti-CRP is fixed on the P3HT active layer channel; the biosensor is prepared by combining a double-dielectric-layer structure and a 3D printing technology; high-selectivity detection of CRP as low as a pM level is realized through physical adsorption of the anti-CRP modified active layer, the anti-CRP does not need to be fixed by a complex modification method, the thin film transistor prepared by the invention is used for detecting C-reactive protein, and the detection limit is as low as 1.8 pmol / L.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a thin film transistor for ultra-low limit detection of C-reactive protein and a preparation method thereof. Background technique [0002] CRP is a protein (acute phase protein) that rises sharply in plasma when the body is injured or infected. It is a non-specific marker of acute diseases and can be used as an inflammatory marker to reflect the physiological state of the body. Studies have shown that when the body is infected and produces inflammation, the concentration of CRP will rise sharply in a short period of time, even reaching 200 μg / ml, but the normal level of CRP is almost 0. In addition, the content of CRP in plasma can also be used to predict the occurrence of cardiovascular disease. When the CRP concentration in serum is between 1-3 μg / ml, the probability of suffering from cardiovascular disease is low, and if it is higher than 3 μg / ml, the probability of sufferin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/414G01N33/68B82Y15/00B82Y40/00
CPCG01N27/4145G01N33/68B82Y15/00B82Y40/00G01N2333/4737
Inventor 李强桑胜波冀健龙张强韩丹乐杨帆李磊磊丁莉峰
Owner TAIYUAN UNIV OF TECH
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