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Application of trichosanthes kirilowii Maxim and semen armeniacae amarae compound extract or refined product in preparation of cosmetics for improving inflammatory aging

A kind of bitter almond and extract technology, which is applied in the field of use of the compound extract or the refined product of Gualou bitter almond in the preparation of cosmetics for improving inflammatory aging, and can solve the limitation of lack of modern drug efficacy research, efficacy and safety data Preliminary research on rodents and other issues to achieve the effect of weak cytotoxicity, high safety and anti-aging effect

Pending Publication Date: 2022-07-01
上海小方制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the pharmacological effects of the two-drug compound on inflammation and aging, and there is a lack of systematic modern pharmacological research on the application of the compound in cosmetics.
[0003] Cannabinoids are widely used in cosmetics and have various activities such as treating acne, skin eczema, and wound healing, but the current effectiveness and safety data are almost entirely limited to preliminary studies on rodents

Method used

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  • Application of trichosanthes kirilowii Maxim and semen armeniacae amarae compound extract or refined product in preparation of cosmetics for improving inflammatory aging
  • Application of trichosanthes kirilowii Maxim and semen armeniacae amarae compound extract or refined product in preparation of cosmetics for improving inflammatory aging
  • Application of trichosanthes kirilowii Maxim and semen armeniacae amarae compound extract or refined product in preparation of cosmetics for improving inflammatory aging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Cell Proliferation Assay

[0054] Take macrophages (THP-1, PBMC, RAW264.7), adjust the cell concentration, and inoculate 5,000 cells per well into a 96-well plate respectively. After the cells are fully adherent, remove the upper medium, and add Fructus Gourmandii almonds. Medium for compound extract, compound extract-1 and compound extract-2 (1μg / mL, 2μg / mL, 4μg / mL, 8μg / mL, 16μg / mL, 32μg / mL, 80μg / mL), separate Blank control group (medium) and negative control group (cells, culture medium). After 24 hours of incubation, 10 μL of LCK-8 solution was added to each well. Continue to incubate for 4h in the cell incubator, and detect the absorbance value at 450nm.

[0055] Survival rate (%)=(absorbance value of administration group-absorbance value of blank group) / (absorbance value of negative control group-blank group)×100%.

[0056] The result is as figure 1 Shown: Gualou bitter almond compound extract and compound extract-1 (1-32 μg / mL) had no obvious effect...

Embodiment 2

[0057] Example 2: Immunoblotting experiment

[0058] Take macrophages (PBMC, THP-1, RAW264.7), adjust the cell concentration to 5 × 10 per well, respectively. 5 The cells were seeded into a 6-well plate, and after the cells were fully adherent, the upper medium was removed, and the compound extract (4μg / mL), compound extract-1 (4μg / mL) and compound extract- 2 (4 μg / mL) medium, and a negative control group and a model group (100 ng / mL LPS) were also set up. After 24 hours of incubation, the cells were collected, and the supernatant was collected by lysing the cells with RIPA strong cell lysis buffer containing PMSF under ice bath conditions. Use SDS-PAGE gel (separating gel 12%, stacking gel 5%) for electrophoresis, and use gel imaging system to acquire images after transfer to membrane.

[0059] The result is as figure 2 Shown: Compared with the control, LPS in the model group increased the expression of cannabinoid CB1 receptors in macrophages and decreased the expression...

Embodiment 3

[0060] Example 3: Calcium ion detection

[0061] Take macrophages (PBMC, THP-1, RAW264.7), adjust the cell concentration to 5 × 10 per well, respectively. 5 The cells were seeded into a 6-well plate, and after the cells were fully adherent, the upper medium was removed, and the compound extract (4μg / mL), compound extract-1 (4μg / mL) and compound extract- 2 (4 μg / mL) medium, and a negative control group and a model group (100 ng / mL LPS) were also set up. After 24 hours of incubation, cells were collected, and 200 μL of sample lysis buffer was added to each well to fully lyse. Centrifuge at 12,000g for 5 minutes at 4°C, and take the supernatant. Prepare the standard solution according to the kit instructions, make the standard curve, and detect the calcium ion content in the sample to be tested.

[0062] The result is as image 3 Shown: The compound extract of Gualou bitter almond, compound extract-1 and compound extract-2 can significantly inhibit LPS-induced calcium influx ...

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Abstract

The invention relates to application of a trichosanthes kirilowii Maxim and semen armeniacae amarae compound extract or a trichosanthes kirilowii Maxim and semen armeniacae amarae compound refined product in preparation of cosmetics and medicines for improving inflammatory aging. The trichosanthes kirilowii Maxim and semen armeniacae amarae compound extract and the compound refined product have weak cytotoxic effect on macrophages and are high in safety. The obvious anti-inflammatory and anti-aging effects are shown on an LPS (Lipopolysaccharide) and H2O2 induced macrophage inflammation and aging model.

Description

technical field [0001] The present invention relates to the use of an extract or a refined product in cosmetics or medicines, in particular, the present invention relates to the use of a compound extract or a refined product of Fructus Fructus Fructus, in the preparation of cosmetics and medicines for improving inflammatory aging . Background technique [0002] In ancient medical books of the past dynasties, melon and bitter almonds are often used as cosmetic formulations. Both are commonly used traditional Chinese medicines with a long history of drug use, and have application value as safe and effective cosmetic active raw materials. Bitter almond, as a medicinal material with the same origin as medicine and food, was first recorded in the Han "Shen Nong's Materia Medica". Although bitter almond is slightly poisonous, all generations have emphasized that bitter almond should be processed and used as medicine to reduce toxicity and enhance efficacy. The processing of almon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61K36/736A61P39/06A61P29/00
CPCA61K8/9789A61Q19/08A61K36/428A61K36/736A61P39/06A61P29/00A61K2800/78A61K2300/00
Inventor 王冰张宁方家辰邹纯朴
Owner 上海小方制药股份有限公司
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