Method for simultaneously determining six related steroid hormones of CAH

A technology of steroid hormones and isotopes, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low positive predictive value, low specificity, and inability to detect effectively, so as to improve specificity, positive predictive value, and improve efficiency Effect

Pending Publication Date: 2022-07-01
普宁市人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the existence of cross-reactivity, the specificity of this technique is low, resulting in a high false positive rate and low posi

Method used

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  • Method for simultaneously determining six related steroid hormones of CAH
  • Method for simultaneously determining six related steroid hormones of CAH
  • Method for simultaneously determining six related steroid hormones of CAH

Examples

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Embodiment 1

[0032] A method for the simultaneous determination of six related steroid hormones in CAH, the six related steroid hormones being 17α-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and cortisol; comprising The following steps:

[0033] Step S1: prepare the mixed internal standard product, quality control product and standard curve product respectively;

[0034] The mixed internal standard is 17α-hydroxyprogesterone isotope internal standard, androstenedione isotope internal standard, 11-deoxycortisol isotope internal standard, 21-deoxycortisol isotope internal standard, and cortisol isotope internal standard. Mixture of standard and corticosterone isotope internal standard; the mixed internal standard includes 25ng of 17α-hydroxyprogesterone isotope internal standard, 25ng of androstenedione isotope internal standard, 12.5ng of 11-deoxycortisol isotope Internal standard, 12.5ng of 21-deoxycortisol isotope internal standard, 25ng of cortisol...

Embodiment 2

[0049] Using the method for simultaneous determination of six related steroid hormones of CAH in Example 1, the quantitative determination results of six related steroid hormones are obtained, which are used to screen two common deficient CAHs of 11-OHD and 21-OHD. The interpretation process is as figure 1 As shown in the figure, Y=Yes, N=No, Negative represents negative, that is, healthy people, Positive represents positive, that is, sick; F1=17α-OHP neonatal threshold is 1.0-2.0, less than F1 is healthy, and greater than F1 Carry out the next step of interpretation; F2=(17α-OHP+A4) / F, the threshold of F2 is 0.05-0.1, greater than F2 is 21-OHD, and less than F2, the next step is to be interpreted; F3=S, the threshold of F3 is 2.0-2.5 , greater than F3 is 11-OHD, less than F3 for next step interpretation; F4=CORT, F4 threshold is 1.0~2.0, less than F4 is 11-OHD, greater than F4 for next step interpretation; F5=21DOC, F5 threshold is 1.0 ~2.0, greater than F5 is 21-OHD, and le...

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Abstract

The invention relates to the field of screening of congenital adrenal hyperplasia, in particular to a method for simultaneously determining six related steroid hormones of CAH. The six related steroid hormones are 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone; the determination method comprises the following steps: S1, respectively preparing a mixed internal standard product, a quality control product and a standard curve product; s2, preparing a sample extracting solution; s3, extracting and incubating a sample; s4, concentrating the redissolved sample; and S5, determining the reconstitution fluid by using a liquid chromatography tandem mass spectrometer, drawing a calibration curve, and calculating the concentrations of 17 alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol and corticosterone in the sample to be detected. The CAH screening specificity and the positive predicted value can be effectively improved. And the detected analyte contains corticosterone, so that 11-OHD and 21-OHD are more effectively distinguished in CAH screening, and the CAH screening efficiency is improved.

Description

technical field [0001] The invention relates to the field of congenital adrenal hyperplasia screening, in particular to a method for simultaneously measuring six CAH-related steroid hormones. Background technique [0002] Congenital adrenal hyperplasia (CAH) is a relatively common autosomal recessive disorder caused by congenital defects in enzymes required for the synthesis of corticosteroids. Insufficient synthesis of cortisol reduces blood concentration. Due to negative feedback, it stimulates the pituitary to secrete adrenocorticotropic hormone, which leads to adrenal hyperplasia and excessive secretion of cortisol precursors, such as 11-deoxycortisol and adrenal androsterone, etc. , resulting in a series of clinical symptoms. The main clinical symptoms are: 1. Salt-losing type, which is the type with the most severe clinical manifestations, which can show severe hyponatremia, lethargy, vomiting and slow weight gain; 2. Simple masculinization, except for no In addition...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N2030/027
Inventor 方炳雄陈福蔡勉珊赵炜田沛润刘琪赵梅
Owner 普宁市人民医院
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