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Tissue culture medium and tissue culture method for rapid propagation of grape rootstock stem

A medium and subculture medium technology, applied in the field of plant cultivation, can solve the problems of unsatisfactory proliferation coefficient and rooting rate, etc.

Active Publication Date: 2022-07-05
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the multiplication coefficient and rooting rate of the current grape tissue culture technology are not ideal

Method used

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  • Tissue culture medium and tissue culture method for rapid propagation of grape rootstock stem
  • Tissue culture medium and tissue culture method for rapid propagation of grape rootstock stem
  • Tissue culture medium and tissue culture method for rapid propagation of grape rootstock stem

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The annual 5BB rootstock seedlings from the Sanping teaching practice base of Xinjiang Agricultural University were used as the material, and the parents were winter grapes × riparian grapes. Planted on the indoor balcony of the Characteristic Fruit Tree Research Center, School of Horticulture, Xinjiang Agricultural University, and cultivated potted seedlings. After about 60 days, the young shoots with a thickness of 1 to 2 mm were grown to 10 nodes for experimental treatment.

[0070] (1) Disinfection of explants: Cut young young shoots into single bud stem segments of 2 to 3 cm long, put them in a beaker, cover with gauze, rinse with running water for 1 hour, and remove surface stains. Then on the ultra-clean workbench, soak in 75% ethanol for 30s, rinse with sterile water for 5 times, then disinfect with 0.1% mercuric solution for 8 minutes, rinse with sterile water for 5 times, and place it in a sterilized inoculation tray for later use. .

[0071] (2) Primary cul...

Embodiment 2

[0075] The annual 1103P rootstock seedlings from the Sanping teaching practice base of Xinjiang Agricultural University were used as the material, and the parents were winter grapes × sand grapes. Planted on the indoor balcony of the Characteristic Fruit Tree Research Center, School of Horticulture, Xinjiang Agricultural University, and cultivated potted seedlings. After about 60 days, it grew to 10 nodes, and the young shoots were taken for experimental treatment.

[0076] Culture according to the method of Example 1, the difference is that MS medium is used as the basic medium in the subculture, and 30g / L sucrose, 7g / L agar, 1.25mg / L 6-BA, 1.0mg / L IBA and 0.5mg / L GA 3 . In the rooting culture, 1 / 2 MS medium was used as the basic medium, and 1.0 mg / L IBA, 0.1 g / LAc, 30 g / L sucrose and 7 g / L agar were added, and the cells were cultured in the dark for 7 days. The growth situation of gained clump bud after the subculture of embodiment 2 sees image 3 . The rooting situatio...

Embodiment 3

[0078] The annual SO4 rootstock seedlings from the Sanping teaching practice base of Xinjiang Agricultural University were used as materials, and their parents were winter grapes × riparian grapes. Planted on the indoor balcony of the Characteristic Fruit Tree Research Center, School of Horticulture, Xinjiang Agricultural University, and cultivated potted seedlings. After about 60 days, it grew to 10 nodes, and the young shoots were taken for experimental treatment.

[0079] Carry out in the manner of Example 1, the difference is that MS medium is used as the basic medium in the subculture, and 30g / L sucrose, 7g / L agar, 2.0mg / L 6-BA, 0.2mg / L IBA and 0.2mg / L GA 3 30g / L sucrose and 7g / L agar. In the rooting culture, 1 / 2 MS medium was used as the basic medium, and 1.5 mg / L IBA, 0.1 g / LAc, 30 g / L sucrose and 7 g / L agar were added, and the cells were cultured in the dark for 7 days. The growth situation of gained clump bud after the subculture of embodiment 3 sees Figure 5 . ...

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Abstract

The invention belongs to the technical field of plant cultivation, and particularly relates to a tissue culture medium and a tissue culture method for rapid propagation of grape rootstock stems. The invention provides a tissue culture medium for rapid propagation of grape rootstock stems. The tissue culture medium comprises a primary culture medium, a subculture medium and a rooting culture medium, the primary culture medium takes an MS culture medium as a basic culture medium, and further comprises 2.0 mg / L of 6-BA, 0.2 mg / L of IBA and 0.2 mg / L of GA3; the subculture medium takes an MS culture medium as a basic culture medium, and further comprises 1.25 mg / L to 2.5 mg / L of 6-BA, 0.2 mg / L to 1.0 mg / L of IBA and 0.2 mg / L to 0.5 mg / L of GA3. According to the invention, the technical problem that the multiplication coefficient and the rooting rate are not ideal during grape tissue culture seedling raising is solved through the matching of the components in the primary culture medium, the subculture medium and the rooting culture medium and the use amount and the relative proportion of the components.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a tissue culture medium and a tissue culture method for rapid propagation of grape rootstock stem segments. Background technique [0002] Grape (Vitis vinifera L.) is a woody vine of the Vitis genus and is one of the oldest fruit tree species in the world. It is native to western Asia and is now widely planted all over the world. . In the 1860s, a widespread pest - grape phylloxera was discovered in California, USA, which had a serious impact on the grape industry all over the world, leading to a fundamental change in the world's viticulture system, with Eurasian species taking root Cultivation is converted to grafting production of resistant rootstocks. To this end, the study found that the selection and utilization of resistant rootstocks have become the key factors to effectively solve the problem of grape production. [0003] The traditional grape pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02A40/22
Inventor 周龙李志慧刘春燕周伟权
Owner XINJIANG AGRI UNIV
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