Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Long-period fiber bragg grating modified based on toxic strain imprinting artificial antibody combined with bacteriophage and preparation method and application of long-period fiber bragg grating

A fiber grating and artificial antibody technology, which is applied in the measurement of phase influence characteristics, instruments, measurement devices, etc., can solve the problems of high price of antibody proteins and low detection efficiency.

Pending Publication Date: 2022-07-05
NINGBO UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antibody proteins are expensive and the analysis time of this method is generally 20-40 minutes. For actual emergency detection, the detection efficiency is still low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Long-period fiber bragg grating modified based on toxic strain imprinting artificial antibody combined with bacteriophage and preparation method and application of long-period fiber bragg grating
  • Long-period fiber bragg grating modified based on toxic strain imprinting artificial antibody combined with bacteriophage and preparation method and application of long-period fiber bragg grating
  • Long-period fiber bragg grating modified based on toxic strain imprinting artificial antibody combined with bacteriophage and preparation method and application of long-period fiber bragg grating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041](1) Preparation of long-period fiber grating: Using femtosecond laser direct writing technology, a high-sensitivity long-period fiber grating was fabricated in the laboratory with a single-mode fiber. Burn the fiber using an optical parametric amplifier system with a center wavelength of 800nm. Femtosecond laser pulses with a repetition rate of 1 kHz and an energy of 0.8 μJ were focused into the fiber core by using a 40x objective with an NA (numerical aperture) of 0.6. A long-period fiber grating with a grating period Λ of 360 μm, a duty cycle of 50% and a total of 500 grating elements is inscribed along the fiber core, and the total grating length is 18 mm. During LPFG inscription, the transmission spectrum evolution from 1100 nm to 1700 nm was monitored in real time using a supercontinuum source and spectrum analyzer. The diameter of the fabricated long-period fiber grating is 114 μm, and the loss peak intensity is 30 dB.

[0042] (2) Surface silanization of the lon...

Embodiment 2

[0049] Using the long-period fiber grating modified by the imprinted artificial antibody of Aeromonas tempera and phage-modified prepared in Example 1 to draw the standard curve with the concentration of Aeromonas tempera as the abscissa and the offset of the resonance wavelength as the ordinate, and detect The content of Aeromonas Temperate in the sample.

[0050] (1) Preparation of the standard mild Aeromonas viable bacteria solution: the concentration of the prepared mild Aeromonas viable bacteria solution is 10 2 , 10 3 , 10 4 , 10 5 , 10 6 , 10 7 CFU / mL, the preparation solvent is PBS buffer solution.

[0051] (2) Recording of initial signal: Immerse the long-period fiber grating sensing area based on the imprinting of artificial antibody and phage modified by Aeromonas mildew obtained in Example 1 in PBS buffer solution, and record the transmission spectrum position, which is recorded as the initial signal position (λ 0 ).

[0052] (3) Target adsorption: immerse ...

Embodiment 3

[0063] The anti-interference ability test of the long-period fiber grating based on the imprinted artificial antibody of Aeromonas mildew combined with phage-modified prepared in Example 1 against other live toxin strains, including Staphylococcus aureus, Salmonella, Listeria bacteria. The concentration of each virulent live strain is 10 7 CFU / mL, the results are as Figure 5 shown. Depend on Figure 5 It can be seen that the long-period fiber grating based on the imprinted artificial antibody of Aeromonas militaris combined with phage-modified long-period fiber grating can only produce obvious resonance wavelength shift for the live Aeromonas solutes, and almost no shift for the other live virulent strains. , indicating that the invention based on Aeromonas mildew imprinted artificial antibody combined with phage-modified long-period fiber grating has strong specificity, and other virulent live strains will not interfere with the detection system.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Diameteraaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a long-period fiber bragg grating modified by combining a toxic strain imprinting artificial antibody with a bacteriophage and a preparation method and application of the long-period fiber bragg grating, and belongs to the technical field of toxic live strain detection. The preparation method of the long-period fiber bragg grating based on toxic strain imprinting artificial antibody combined with bacteriophage modification comprises the following steps: sequentially carrying out surface silanization treatment, polydopamine coating modification, toxic strain imprinting artificial antibody modification, bacteriophage modification and sealing on the long-period fiber bragg grating; and obtaining the long-period fiber bragg grating modified by combining the toxic strain imprinting artificial antibody with the bacteriophage. The toxic strain imprinting artificial antibody prepared by the method is combined with the bacteriophage modified long-period fiber bragg grating, so that the detection time can be shortened to 5-10 minutes, the sensitivity of detecting specific toxic live strains is improved, and the method has the advantages of high sensitivity, high accuracy, strong specificity, low detection limit and environmental protection.

Description

technical field [0001] The invention relates to the technical field of detection of live toxins, in particular to a long-period fiber grating modified by artificial antibodies imprinted on toxins combined with phage, and a preparation method and applications thereof. Background technique [0002] Microorganisms are ubiquitous in nature, they are present in drinking water, fruits and other foods. The widespread presence of pathogenic strains can lead to major epidemics and diseases. For example, staphylococcal disease is an acute or chronic infectious disease caused by Staphylococcus aureus, and clinically it has various types, such as arthritis, tenosynovitis, swelling of foot pads, umbilicus, and staphylococcal sepsis. Bacteria-contaminated water and food are a major source of microbial-mediated infections in both developed and developing countries. Therefore, it is necessary to develop a sensitive, rapid, and on-site detection method for virulent live strains to achieve ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/41G01N33/53G01N33/531
CPCG01N21/41G01N33/53G01N33/531
Inventor 朱珊珊干宁张培晴戴世勋曹玉廷李天华
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com