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Magnetic core-shell structure porous silicon dioxide carrier for laccase immobilization as well as preparation method and application of magnetic core-shell structure porous silicon dioxide carrier

A technology of porous silica and core-shell structure, applied in the directions of silica, silica, etc., which are fixed on or in an inorganic carrier, can solve the problems of inability to achieve repeated use, poor long-term preservation, and easy shedding of enzymes, etc. Achieving the effect of wide pH and temperature applicable range, excellent performance, and simple preparation method

Active Publication Date: 2022-07-08
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these carriers have a high loading capacity and are relatively stable after immobilization, they also have some disadvantages: (1) poor long-term storage; (2) easy to fall off after enzyme immobilization; (3) unable to realize repeated use, etc.

Method used

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  • Magnetic core-shell structure porous silicon dioxide carrier for laccase immobilization as well as preparation method and application of magnetic core-shell structure porous silicon dioxide carrier
  • Magnetic core-shell structure porous silicon dioxide carrier for laccase immobilization as well as preparation method and application of magnetic core-shell structure porous silicon dioxide carrier
  • Magnetic core-shell structure porous silicon dioxide carrier for laccase immobilization as well as preparation method and application of magnetic core-shell structure porous silicon dioxide carrier

Examples

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preparation example Construction

[0040] A preparation method of magnetic silica microspheres with core-shell structure, the method comprises the following steps:

[0041] (1) The magnetic Fe 3 O 4 Disperse in a mixed solution consisting of ethanol, deionized water and concentrated ammonia, and ultrasonicate for 30 min to obtain a suspension. TEOS was then added dropwise to the suspension and stirring was continued for 6 h in a 30°C water bath. After the reaction, the product was separated with a magnet, washed and dried to obtain Fe 3 O 4 @d-SiO 2 . TEOS and Fe 3 O 4 The mass ratio of (0.1-0.3) g: (0.05-0.15) g.

[0042] (2) Fe 3 O 4 @d-SiO 2 Disperse in a mixed solution containing CTAB, ethanol, deionized water and concentrated ammonia, and stir for 30 min to form a homogeneous solution. Under continuous stirring, TEOS was added dropwise to the above solution and stirred continuously for 6 h in a 30 °C water bath. After the reaction, the product was separated with a magnet and washed several tim...

Embodiment 1

[0047] A preparation method of a magnetic core-shell structure porous silica carrier, the method comprises the following steps:

[0048] (1) 0.1g of magnetic Fe 3 O 4 Dispersed in a mixed solution consisting of 120 mL of ethanol, 40 mL of deionized water and 4 mL of concentrated ammonia water, and ultrasonicated for 30 min to obtain a suspension. Then 0.2 g of TEOS was added dropwise to the suspension, and stirring was continued for 6 h in a 30° C. water bath. After the reaction, the product was separated with a magnet, washed and dried to obtain Fe 3 O 4 @d-SiO 2 .

[0049] (2) 0.15g Fe 3 O 4 @d-SiO 2 Disperse in a mixed solution containing 0.3 g CTAB, 60 mL ethanol, 80 mL deionized water and 1 mL concentrated ammonia water, and stir for 30 min to form a homogeneous solution. Under continuous stirring, 0.4 g of TEOS was added dropwise to the above solution, and stirred continuously for 6 h in a water bath at 30 °C. After the reaction, the product was separated with ...

Embodiment 2

[0057] A preparation method of a magnetic core-shell structure porous silica carrier, the method comprises the following steps:

[0058] (1) 0.05g magnetic Fe 3 O 4 Dispersed in a mixed solution consisting of 120 mL of ethanol, 40 mL of deionized water and 4 mL of concentrated ammonia water, and ultrasonicated for 30 min to obtain a suspension. Then 0.1 g of TEOS was added dropwise to the suspension, and stirring was continued for 6 h in a 30°C water bath. After the reaction, the product was separated with a magnet, washed and dried to obtain Fe 3 O 4 @d-SiO 2 .

[0059] (2) 0.1g Fe 3 O 4 @d-SiO 2 Disperse in a mixed solution containing 0.3 g CTAB, 60 mL ethanol, 80 mL deionized water and 1 mL concentrated ammonia water, and stir for 30 min to form a homogeneous solution. Under continuous stirring, 0.3 g of TEOS was added dropwise to the above solution, and stirred continuously for 6 h in a 30 °C water bath. After the reaction, the product was separated with a magnet...

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Abstract

The invention relates to a magnetic core-shell structure porous silicon dioxide carrier for laccase immobilization and a preparation method and application thereof.Magnetic Fe3O4 microspheres serve as a core of the carrier, then the surface of the magnetic Fe3O4 microspheres is coated with a thin layer of compact SiO2 (d-SiO2) through a sol-gel method, finally the surface of the magnetic Fe3O4 microspheres is continuously coated with a layer of porous SiO2 (p-SiO2) through a template method, and the magnetic core-shell structure porous silicon dioxide carrier for laccase immobilization is obtained. The magnetic porous silicon dioxide (Fe3O4 (at) d-SiO2 (at) p-SiO2) microspheres with the core-shell structure are obtained. Porous Fe3O4 (at) d-SiO2 (at) p-SiO2 is used as a carrier, and laccase is immobilized through an adsorption method. Compared with free laccase, the immobilized laccase (Fe3O4 (at) d-SiO2 (at) p-SiO2-Lac) is better in stability, has higher catalytic activity, can be repeatedly used, and has a wide application prospect in the aspect of bioremediation of organic contaminated soil.

Description

technical field [0001] The invention relates to the field of laccase immobilization carriers, in particular to a magnetic core-shell structure porous silica carrier for laccase immobilization and a preparation method and application thereof. Background technique [0002] In recent years, along with the relocation and reconstruction of a large number of industrial enterprises, the remediation of organically polluted soil has become a problem that people pay close attention to. Compared with traditional physical and chemical remediation techniques, microbial remediation has many advantages such as low cost, good effect, and no harmful residues. Compared with microbial remediation, enzymatic remediation is less restricted by soil environmental factors and is not sensitive to competition between microorganisms. [0003] Among them, laccase is a copper ion-containing polyphenol oxidase, which exhibits excellent properties in the degradation of various organic pollutants and is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08C01B33/12C12N9/02C12N11/14
CPCC01G49/08C01B33/12C12N11/14C12N9/0061C12Y110/03002C01P2004/82C01P2006/42C01P2004/62C01P2006/12C01P2004/04C01P2004/03C01P2002/82C01P2002/72C01P2006/17Y02W10/10
Inventor 胡晓钧闫家琪董文雅杨曜宇
Owner SHANGHAI INST OF TECH
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