Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano cellulose as well as preparation method and application thereof

A technology of nanocellulose and cellulose, applied in the field of enzyme engineering, can solve the problem that nanocellulose does not have functional groups, and achieve the effects of low cost, wide application prospects and mild reaction conditions

Pending Publication Date: 2022-07-08
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of preparing nanocellulose is to crush natural cellulose through high-power mechanical pulverization and strong acid-base treatment, but the nanocellulose obtained by this method does not have any functional groups except hydroxyl groups.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano cellulose as well as preparation method and application thereof
  • Nano cellulose as well as preparation method and application thereof
  • Nano cellulose as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] like figure 2 As shown, a preparation method of nanocellulose, the specific steps are as follows:

[0038] (1) In the whole reaction system, add 50g / L soluble starch, 10mM arbutin primer, 20mM inorganic phosphorus (by KH 2 PO 4 and K 2 HPO 4 prepared), 10mM magnesium ion, 2g / LαGP (amino acid sequence NCBI accession number is WP_011250357.1) and 2g / L CDP (amino acid sequence NCBI accession number is WP_020457986.1), 200mM HEPES (pH7.5) buffer solution , put the whole system at 45°C in a water bath to synthesize nanocellulose;

[0039] (2) The nanocellulose obtained in step (1) was washed, centrifuged and purified 10 times with HEPES solution (same as in step (1)), and then freeze-dried.

[0040] The characterization of the novel nanocellulose synthesized by the enzyme-catalyzed method in the examples is as follows: figure 1 shown. Figure (a) shows the new nanocellulose 1 H NMR data. 4.35ppm is expressed as the 1-position hydrogen (H) in the glucose structural u...

Embodiment 2

[0044] A preparation method of nanocellulose, the specific steps are as follows:

[0045] (1) In the whole reaction system, add 100g / L soluble amylose, 50mM arbutin primer, 40mM inorganic phosphorus (by NaH 2 PO 4 and Na 2 HPO 4 prepared), 10mM magnesium ion, 5g / LαGP and 2g / L CDP, 600mM HEPES (pH7.5) buffer solution, put the whole system at 40°C in a water bath to synthesize nanocellulose;

[0046] (2) The nanocellulose obtained in step (1) was washed, centrifuged and purified 10 times with HEPES solution (same as in step (1)), and then freeze-dried.

Embodiment 3

[0048] A preparation method of nanocellulose, the specific steps are as follows:

[0049] (1) In the whole reaction system, add 10g / L soluble starch, 1mM arbutin primer, 1mM inorganic phosphorus (by KH 2 PO 4 prepared), 10mM magnesium ion, 1g / LαGP and 0.1g / L CDP, 10mM HEPES (pH 7.5) buffer solution, the whole system was placed at 45°C in a water bath to synthesize nanocellulose;

[0050] (2) The nanocellulose obtained in step (1) was washed, centrifuged and purified 10 times with HEPES solution (same as in step (1)), and then freeze-dried.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
widthaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides nanocellulose as well as a preparation method and application thereof, the nanocellulose endows the nanocellulose with a new function, is novel nanocellulose with a functional group, and can be used as an emulsifier; according to the preparation method, arbutin with a functional group is selected as a primer, starch is selected as a substrate, the novel nanocellulose is prepared under the catalytic action of an in-vitro enzyme, the preparation process of the enzyme synthesized cellulose is simple, the reaction condition is environment-friendly, and the defects of high energy consumption, environmental pollution and the like in the existing nanocellulose preparation process are well overcome.

Description

technical field [0001] The invention relates to the field of enzyme engineering, in particular to a nanocellulose and a preparation method and application thereof. Background technique [0002] As a new type of nanomaterial, nanocellulose not only has the characteristics of high specific surface area, but also has many advantages such as low density, low cost, high strength, renewable and biodegradable, and is widely used in coatings. , electronic devices and composite materials. The traditional preparation method of nanocellulose is to pulverize natural cellulose with high power and treat with strong acid and alkali, but the nanocellulose obtained by this method does not have any functional groups except hydroxyl groups. [0003] The molecular chain structure of nanocellulose prepared by in vitro enzymatic method is precise, which has obvious advantages over chemical synthesis (Chemical Reviews 2016, 116, 2307-2413). The whole enzyme-catalyzed reaction process is complete...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
CPCC12P19/04
Inventor 游淳李强子
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com