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Mucous membrane melanoma 3D (three-dimensional) organ as well as culture method and application thereof

A technology for melanoma and culture methods, applied in biochemical equipment and methods, tumor/cancer cells, animal cells, etc., can solve problems such as lack of reference experience, and achieve high consistency in maintaining specificity, tissue morphology and genotyping Effect

Pending Publication Date: 2022-07-12
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current preclinical models of oral mucosal melanoma have been reported, such as cell lines, PDC and PDX, there is still a lack of effective reference experience for the construction of oral mucosal melanoma organoid models

Method used

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  • Mucous membrane melanoma 3D (three-dimensional) organ as well as culture method and application thereof
  • Mucous membrane melanoma 3D (three-dimensional) organ as well as culture method and application thereof
  • Mucous membrane melanoma 3D (three-dimensional) organ as well as culture method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0132] Example 1 Mucosal melanoma organoid culture

[0133] The source of the specimen was the successfully constructed mucosal melanoma PDX tumor tissue in previous studies ( Figure 5 ). The construction of PDX refers to the existing method of the present inventor's research group, that is, patient-derived biopsy or surgical tissue is subcutaneously inoculated into immunodeficient mice. The specific operation is to pour an appropriate amount of 0.04% sodium hypochlorite into a disposable sterile petri dish, and add 1% penicillin-streptomycin double antibody PBS or three serum-free medium. Take out the isolated mucosal melanoma tumor tissue, wash it in sodium hypochlorite solution for 10 seconds, quickly put it into PBS or serum-free medium to wash off the sodium hypochlorite on the tumor surface, trim the surface necrosis and fibrous tissue of the tumor tissue with sterile instruments, and replace with Wash twice with PBS or serum-free medium for 2 minutes each time, and c...

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Abstract

The invention belongs to the field of biological medicines, and discloses a mucous membrane melanoma 3D (three-dimensional) organ as well as a culture method and application thereof. The invention further discloses a culture medium for culturing the mucosal melanoma 3D organoid, and the mucosal melanoma cell organoid can be effectively formed in a 3D environment by selecting specific culture medium components according to phenotypic characteristics of mucosal melanoma cells and compounding the components according to a certain proportion. The mucous membrane melanoma organ obtained by the culture medium and the method can be used for drug screening, action target research and the like, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of organoid culture, and relates to a mucosal melanoma 3D organoid and a culture method and application thereof. Background technique [0002] Mucosal melanoma (MM) is a rare subtype of aggressive melanoma that can occur in the oral cavity, oropharynx, nasal cavity / sinus, esophagus, rectal anal canal, reproductive tract, urinary tract and other mucosal tissues middle. MM accounts for about 1% of the total melanoma. Compared with skin melanoma, melanomas that occur in the head and neck mucosa have a higher degree of malignancy, high recurrence rate and distant metastasis rate, poor prognosis and high mortality rate. Most reports showed a 5-year survival rate of less than 25%. [0003] The incidence of melanoma has a certain ethnic specificity: Caucasians are more prone to cutaneous malignant melanomas, and mucosal melanomas are relatively rare; while yellow people have more common mucosal and acral melanoma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071C12Q1/02
CPCC12N5/0693C12N5/0626G01N33/5011G01N33/5076C12N2513/00C12N2500/32C12N2501/11C12N2500/38C12N2501/15C12N2501/415C12N2503/02C12N2501/727C12N2501/119C12N2501/115C12N2501/02C12N2501/01C12N2501/39G01N2500/10
Inventor 石超吉顾子悦周榕陈宛灵徐声铭韩永张志愿
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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