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LC-MS/MS detection method for gallotannin in plants

A detection method and gallium technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ion saturation, narrowing the scanning range, limiting the use, etc., and achieve the effects of strong signal, reduced chemical background, and high sensitivity

Pending Publication Date: 2022-07-12
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Among them, non-targeted detection technologies represented by time-of-flight (TOF) and orbitrap (Orbitrap) can scan the ion information of metabolites, and the mass analysis results have ultra-high resolution. Ion saturation occurs, limiting its use in accurate quantitative detection of metabolites
Targeted detection technology represented by triple quadrupole (QQQ) can purposely narrow the scanning range, thereby improving the repeatability and sensitivity of target metabolite detection, combined with the multi-stage reaction monitoring (MRM) mode is widely used in metabolic Quantitative analysis of metabolites, but targeted mass detection of metabolites requires optimization of MRM conditions using known chemical standards

Method used

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  • LC-MS/MS detection method for gallotannin in plants
  • LC-MS/MS detection method for gallotannin in plants
  • LC-MS/MS detection method for gallotannin in plants

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Embodiment 1

[0085] Peony stamens, peony leaves, peony young stems, and peony root bark were taken as gallic tannin test samples. The test samples were quick-frozen in liquid nitrogen and ground to powder (particle size: 45 mesh). Weigh the ground samples from different tissue parts respectively, add methanol solution (concentration: 80%) in the ratio of 1g:10mL, and extract with ultrasonic (ultrasonic power: 500W, frequency: 40kHz) for 30min. Centrifuge at 4 °C and 10000 rpm for 10 min. After centrifugation, the supernatant was aspirated, filtered with a 0.22 μm filter, and then placed in a -80 °C refrigerator to obtain the sample to be tested.

[0086] The sample to be tested was separated by liquid chromatography, and the liquid chromatography was separated by Shimadzu LC-20A high performance liquid chromatograph. The sample volume is 2 μL, the mobile phase includes mobile phase A and mobile phase B, mobile phase A is formic acid solution with a mass concentration of 1%, mobile phase B ...

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Abstract

The invention belongs to the technical field of compound detection, and provides an LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry) detection method for gallotannin in plants, which comprises the following steps: mixing a plant gallotannin sample and a methanol solution, and sequentially extracting and centrifuging to obtain a sample to be detected; carrying out liquid chromatography separation and tandem mass spectrometry detection on a to-be-detected sample, and establishing a mass spectrometry database of gall tannin compounds; mRM quantitative ion pairs are obtained through screening according to MS / MS information, and optimized MRM mass spectrum conditions are obtained through a plant gallnut tannin sample; ion pairs and retention time of gallotannin compounds are integrated, and MRM mass spectrum conditions are optimized, so that qualitative and quantitative detection of gallotannin in plants is realized. According to the detection method disclosed by the invention, the detection process of the gallotannin compound does not depend on a chemical standard substance, the detection method has the advantages of comprehensive detection, high sensitivity, good repeatability, high accuracy, simplicity in operation and the like, and qualitative and quantitative analysis is realized at the same time.

Description

technical field [0001] The invention relates to the technical field of compound detection methods, in particular to an LC-MS / MS detection method for gallotannins in plants. Background technique [0002] Tannins are an important class of phenolic compounds, which can be divided into condensed tannins and hydrolyzed tannins according to their hydrolyzability. Gall tannins (GTs) are a series of hydrolyzed tannins whose molecular structure usually takes polyols (saccharides) as the core and several gallic acids are bound around them in the form of ester bonds. Gallic tannins are widely found in the roots, stems, leaves, flowers and fruits of dicotyledonous plants such as gall, hyssop, purple-backed longhorn, and peony. Studies have shown that gallotannin compounds have multiple biological activities such as antioxidant, antibacterial, anti-inflammatory, anti-obesity, anti-diabetic, and cardioprotective, so they are widely used in food, health products, medicine and cosmetics. ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/72
CPCG01N30/02G01N30/06G01N30/54G01N30/72
Inventor 白章振张延龙郑田恬于蕊
Owner NORTHWEST A & F UNIV
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