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Microsecond pulse reversible electroporation system for improving concentration of molecules in cells

A microsecond pulse and electroporation technology, which is applied to the acquisition/recognition of microscopic objects, enzymatic/microbiological devices, methods for stress-stimulated microbial growth, etc., can solve the problem of weak killing effect on cancer cells and achieve the elimination of exogenous Effects of interference, promotion, and increase in secretion

Pending Publication Date: 2022-07-15
ZHENGZHOU CHIZHIHENG ELECTRONICS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the killing effect of the active ingredients of traditional Chinese medicine on cancer cells is very weak and has a significant time dependence.

Method used

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  • Microsecond pulse reversible electroporation system for improving concentration of molecules in cells
  • Microsecond pulse reversible electroporation system for improving concentration of molecules in cells
  • Microsecond pulse reversible electroporation system for improving concentration of molecules in cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] like Figure 1-3 As shown, a microsecond pulse reversible electroporation system for increasing the concentration of molecules in cells includes a microsecond pulse generating device and a signal generator, the signal generator is connected with the microsecond pulse generating device, and the signal generator sends a trigger signal , the microsecond pulse generating device is stimulated by a trigger signal to send out a pulse signal, the microsecond pulse device is connected with a motor cup, the motor cup is filled with cell suspension, and the microsecond pulse generating device penetrates the cells through the motor cup; the motor A current probe and a high-voltage probe are arranged on the wire between the cup and the microsecond pulse generating device. The current probe and the high-voltage probe are both connected with an oscilloscope. The cell breakdown current and breakdown voltage are extracted by the oscilloscope, and the breakdown current is extracted. and ...

Embodiment 2

[0047] like Figure 4-7 As shown, on the basis of Example 1, in the process of cell suspension perforation, in order to further promote the effect of the microsecond pulse and the active ingredients of traditional Chinese medicine on tumor cells, increase the apoptosis rate of tumor cells, and improve the effect of drugs on tumor cells. Inhibitory effect of cell activity, in cells with hydrophilic micropores formed by electroporation in the cell suspension, the perforation area A of the micropores satisfies, A=λ·πr 2 dS / 2; in the above formula, λ is the relationship coefficient, the value range is 0.36-0.88; r is the cell radius, d is the cell spacing, and S is the cell surface area. After cell breakdown, the cell membrane tension coefficient δ changes, and the cell membrane tension coefficient is related to the cell perforation area. The tension coefficient δ of the cell membrane satisfies the perforation area A, δ=2δ 1 -((2δ 1 -δ 0 ) / (1-A / S)); in the above formula, δ 1 ...

Embodiment 3

[0055] On the basis of Example 1, the processing method of the system includes: step 1, centrifuging the tumor cells, then directly adding the medicated culture medium of the corresponding concentration set in the experimental group, and putting them into the electric shock cup for electroporation treatment; Step 2, immediately after the treatment in step 1, it was added to each duplicate well of the 96-well plate, and then the medicated medium with the same drug concentration was added to supplement the volume to 100 μl / well, incubated for 24 hours, and then the follow-up detection was carried out; During the process and subsequent incubation, the cells are in the environment of the drug concentration set in the experiment.

[0056]The processing method of the data processing module includes: step 1, the reference data set uses the center of the cell as a label, preprocesses the sub-graph containing only a single center point as the cell label, and obtains the sub-graph contai...

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Abstract

The microsecond pulse reversible electroporation system comprises a microsecond pulse generation device and a signal generator, the signal generator is connected with the microsecond pulse generation device, the signal generator sends out a trigger signal, the microsecond pulse generation device is excited by the trigger signal to send out a pulse signal, and the pulse signal is transmitted to the microsecond pulse generation device. The microsecond pulse generating equipment is connected with a motor cup, a cell suspension is filled in the motor cup, and the microsecond pulse generating equipment breaks down cells through the motor cup; a current probe and a high-voltage probe are arranged on a wire between the motor cup and the microsecond pulse generation equipment, the current probe and the high-voltage probe are both connected with an oscilloscope, and the oscilloscope is used for carrying out feature extraction on cell breakdown current and breakdown voltage to extract time domain and frequency domain information of the breakdown current and the breakdown voltage; the influence on tumor cells when the microsecond pulse and the traditional Chinese medicine effective components jointly act is promoted, the apoptosis rate of the tumor cells is increased, and the activity inhibition effect of the medicine on the tumor cells is improved.

Description

technical field [0001] The invention belongs to the field of medical auxiliary equipment, in particular to a microsecond pulse reversible electroporation system for increasing the concentration of molecules in cells. Background technique [0002] Since anhydrous alcohol injection was used in the treatment of liver tumors in the 1970s, techniques including radiofrequency ablation, microwave ablation, and cryoablation have been rapidly applied in the treatment of various malignant tumors. in palliative care patients who cannot receive complete tumor resection for these reasons. With the development of imaging technology, these percutaneous minimally invasive techniques have improved the prognosis of patients with only limited damage, indicating that thermal ablation techniques such as radiofrequency ablation and microwave ablation have shown good efficacy in clinical treatment. . However, these techniques still have problems such as lack of selectivity for tissue destruction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/34C12M1/00G06V20/69G06V10/82G06N3/04
CPCC12M35/02C12M41/36G06N3/045
Inventor 陈传亮张建华吕彦鹏杨延钧李颖越金雯雯陈科元
Owner ZHENGZHOU CHIZHIHENG ELECTRONICS TECH CO LTD
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