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Olfactory ensheathing cell photo-curing nerve repair hydrogel as well as preparation method and application thereof

A technology for olfactory ensheathing cells and nerve repair, applied in the field of olfactory ensheathing cell photocured nerve repair hydrogel and its preparation, can solve the problems of damaged spinal cord treatment effect, low survival rate, poor quality, etc., achieve good repair effect, improve Survival rate, effect of inducing nerve fiber regeneration

Pending Publication Date: 2022-07-22
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems existing in the above-mentioned prior art, provide a kind of olfactory ensheathing cell light-cured nerve repair hydrogel and its preparation method and application, to solve the existing olfactory ensheathing cell in the actual transplantation treatment process, Low survival rate and poor response to treatment of injured spinal cord

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  • Olfactory ensheathing cell photo-curing nerve repair hydrogel as well as preparation method and application thereof
  • Olfactory ensheathing cell photo-curing nerve repair hydrogel as well as preparation method and application thereof
  • Olfactory ensheathing cell photo-curing nerve repair hydrogel as well as preparation method and application thereof

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Embodiment Construction

[0039] Below in conjunction with accompanying drawing, the present invention is described in further detail:

[0040] The invention provides a preparation method of olfactory ensheathing cell light-cured nerve repair hydrogel, which specifically includes the following steps:

[0041] Step 1, preparing a hydrogel composite scaffold;

[0042] In step 1, the hydrogel composite scaffold is prepared, and the specific operations are as follows:

[0043] Step 11, the preparation mass concentration is 10% methacrylic anhydride gelatin GelMA (sterile water used here), which is solid at 25° room temperature, heated in a 100° water bath for 30s, and GelMA is liquid after heating ; Dilute to a concentration of 5% with a special culture medium for olfactory ensheathing cells, and adjust the pH value to 6.5-7.5 with 40 mmol / L NaOH solution, for later use;

[0044] Step 12, prepare polyether F127 diacrylate (sterilized water used here) with a mass concentration of 40%, which is solid at ro...

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Abstract

The invention provides a preparation method of olfactory ensheathing cell photocuring nerve repair hydrogel. The preparation method specifically comprises the following steps: preparing a hydrogel composite scaffold; mixing the hydrogel composite scaffold with a DMEM / F-12 culture medium containing fetal calf serum to obtain a composite culture medium; and culturing the olfactory ensheathing cells by using the composite culture medium to obtain the olfactory ensheathing cell light-cured neural restoration hydrogel. Compared with traditional olfactory ensheathing cells, the olfactory ensheathing cell photo-curing nerve repair hydrogel provided by the invention can effectively improve the survival rate of the olfactory ensheathing cells, also can fill spinal cord tissue defects and integrate with peripheral tissues, inhibit inflammatory response and glial scar formation, induce nerve fiber regeneration, and improve the survival rate of the olfactory ensheathing cells. The recovery of the motion function after spinal cord injury can be promoted to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering materials and preparation, and in particular relates to an olfactory ensheathing cell light-cured nerve repair hydrogel and a preparation method and application thereof. Background technique [0002] In spinal cord injury, synaptic connections are lost, demyelination and axonal damage disrupt signaling, and neurons suffer primary mechanical damage, secondary to inflammation, lipid peroxidation, ischemia, free radical production , glial scarring, axonal demyelination, cell necrosis and apoptotic cell destruction, etc., seriously inhibit the repair, regeneration and myelination of nerve function. [0003] For nerve fiber regeneration, pathway reconstruction and functional recovery in spinal cord injury, a commonly used treatment method is to implant differentiated glial cells, such as olfactory ensheathing cells (OECs), which is beneficial to nerve regeneration. Olfactory ensheathing cel...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/38A61L27/22C12N5/079
CPCC12N5/0622A61L27/52A61L27/3895A61L27/3878A61L27/3804A61L27/222C12N2533/54C12N2533/30A61L2430/32
Inventor 郭栋贺西京杨于冰程志坚李家良李嘉熙谭清华王放常素娥
Owner XI AN JIAOTONG UNIV
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