Carboxyethyl chitosan derivative/oxidized konjac mannan injectable hydrogel as well as preparation method and application thereof

A technology of konjac mannan and carboxyethyl chitosan, which is applied in application, surgery, surgical adhesives, etc., can solve the problems of limited application and poor water solubility, and achieve excellent tissue adhesion, prevent tissue adhesion, and excellent Effect of anti-infective properties

Active Publication Date: 2022-07-29
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its strong intermolecular hydrogen bonds lead to its poor water solubility, which limits its further application

Method used

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  • Carboxyethyl chitosan derivative/oxidized konjac mannan injectable hydrogel as well as preparation method and application thereof
  • Carboxyethyl chitosan derivative/oxidized konjac mannan injectable hydrogel as well as preparation method and application thereof
  • Carboxyethyl chitosan derivative/oxidized konjac mannan injectable hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A carboxyethyl chitosan derivative / oxidized konjac mannan injectable hydrogel is carried out according to the following steps:

[0031] S1: Dissolve 0.4g chitosan powder (2.5mmol) in 20ml deionized water containing 3.4ml acrylic acid (5mmol) solution, stir until the solution is clear and transparent and add 0.114ml 2-dodecyl acrylate (0.4mmol) , after 3 days of reaction at 40°C, the reaction solution was put into a dialysis bag (MW=8000), firstly dialyzed with absolute ethanol for 3 days, then with deionized water for 3 days, and finally freeze-dried to obtain dodecyl-modified carboxyethyl Chitosan (DCEC);

[0032] S2: Dissolve 1 g of konjac mannan (6.25 mmol) in 100 ml of water, add 0.67 g of sodium periodate (3.125 mmol), react for 5 h at 25°C in the dark, add 1.5 ml of ethylene glycol, and continue the reaction for 1 h. The solution was put into a dialysis bag (MW=8000), and lyophilized after 3 days of deionized water dialysis to obtain oxidized konjac mannan (OKGM)...

Embodiment 2

[0042] A carboxyethyl chitosan derivative / oxidized konjac mannan injectable hydrogel is carried out according to the following steps:

[0043] S1: Dissolve 0.4g chitosan powder (2.5mmol) in 20ml deionized water containing 3.4ml acrylic acid (5mmol) solution, stir until the solution is clear and transparent, add 0.085ml 2-dodecyl acrylate (0.31mmol) After 3 days of reaction at 40°C, the reaction solution was put into a dialysis bag (MW=8000), firstly dialyzed with absolute ethanol for 3 days, then with deionized water for 3 days, and finally freeze-dried to obtain dodecyl-modified carboxyethyl Chitosan (DCEC);

[0044] S2: Dissolve 1 g of konjac mannan (6.25 mmol) in 100 ml of water, add 0.67 g of sodium periodate (3.125 mmol), add 1.5 ml of ethylene glycol after reacting for 5 h at 25°C in the dark, and continue the reaction for 1 h. The solution was put into a dialysis bag (MW=8000), and lyophilized after 3 days of deionized water dialysis to obtain oxidized konjac mannan (O...

Embodiment 3

[0048] A carboxyethyl chitosan derivative / oxidized konjac mannan injectable hydrogel is carried out according to the following steps:

[0049] S1: Dissolve 0.4g chitosan powder (2.5mmol) in 20ml deionized water containing 3.4ml acrylic acid (5mmol) solution, stir until the solution is clear and transparent and add 0.114ml 2-dodecyl acrylate (0.4mmol) , after 3 days of reaction at 40°C, the reaction solution was put into a dialysis bag (MW=8000), firstly dialyzed with absolute ethanol for 3 days, then with deionized water for 3 days, and finally freeze-dried to obtain dodecyl-modified carboxyethyl Chitosan (DCEC);

[0050] S2: Dissolve 1 g of konjac mannan (6.25 mmol) in 100 ml of water, add 1.34 g of sodium periodate (6.25 mmol), react for 5 h at 25°C in the dark, add 1.5 ml of ethylene glycol, continue to react for 1 h and then react The solution was put into a dialysis bag (MW=8000), and lyophilized after 3 days of deionized water dialysis to obtain oxidized konjac mannan (...

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PUM

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Abstract

The invention provides a carboxyethyl chitosan derivative / oxidized konjak mannan injectable hydrogel and a preparation method and application thereof. The preparation method comprises the following steps: mixing a dodecyl modified carboxyethyl chitosan solution and an oxidized konjak mannan solution according to a volume ratio of (3-5): 1; the carboxyethyl chitosan derivative / oxidized konjak mannan injectable hydrogel is obtained. The carboxyethyl chitosan derivative / oxidized konjak mannan injectable hydrogel prepared by the invention has dual effects of closing wounds to realize hemostasis and preventing post-operation tissue adhesion as a physical barrier in the aspect of hemostasis, and also has excellent tissue adhesion, blood cell coagulation hemostasis and cell recruitment functions.

Description

technical field [0001] The invention relates to the technical field of biological materials, and more particularly to a carboxyethyl chitosan derivative / oxidized konjac mannan injectable hydrogel and a preparation method and application thereof. Background technique [0002] Excessive blood loss and hemorrhagic shock are the main causes of traumatic death, accounting for as high as 30-40%. The high mortality rate due to uncontrolled bleeding suggests that when an accident occurs, effective hemostasis should be administered promptly to the patient. In addition to accidents, bleeding from surgery (such as liver surgery, abdominal surgery, etc.) cannot be ignored in medical situations. Improper treatment can lead to adverse consequences such as prolonged operation time and increased blood transfusion. In major surgery, excessive blood loss can even lead to death. Additionally, infection from bleeding may cause an inflammatory response and slow wound healing. Therefore, in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/04A61L24/00A61L31/04A61L31/14C08J3/075C08L5/08C08L5/02C08B37/08C08B37/02
CPCA61L24/043A61L24/0031A61L24/001A61L31/041A61L31/042A61L31/145A61L31/14C08J3/075C08B37/003C08B37/009A61L2400/06A61L2400/04C08J2305/08C08J2405/02C08L5/08C08L5/02
Inventor 杨建海魏祥雨刘文广
Owner TIANJIN UNIV
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