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Rice gene OsSMG6 and application thereof

A gene and rice technology, applied in the fields of genetic engineering and plant genetic breeding, can solve the problems of slow growth of large-scale rice production

Active Publication Date: 2022-08-02
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the past two decades, due to the limited germplasm resources in my country, the large-scale rice yield has grown slowly (Yang Shihua et al., 2010). With the continuous growth of my country's population and the decrease of arable land, how to achieve the third leap in rice yield It is a new challenge facing rice research institutes today

Method used

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  • Rice gene OsSMG6 and application thereof
  • Rice gene OsSMG6 and application thereof
  • Rice gene OsSMG6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Gene selection and material creation

[0059] Epigenetic modification is a heritable mechanism that regulates gene expression levels formed during the evolution of multicellular organisms. This genetic mechanism functions throughout an organism's life. Epigenetic modifications include DNA methylation, histone modification and chromatin remodeling. Histone modifications can be divided into histone acetylation, methylation, phosphorylation, ubiquitination and SUMOylation, which change chromatin structure. Regulate gene expression. In particular, histone methylation and demethylation modifications play an important role in panicle development. It regulates the level of methylation modification of genes H3K4, H3K27 and H3K36, promotes or inhibits the expression of downstream target genes, and participates in regulating the growth and development of rice panicle meristems, thereby changing rice grain and yield.

[0060] Histone modification mainly occurs on hist...

Embodiment 2

[0072] Example 2 Grain phenotype detection

[0073] The grain type and grain weight of wild-type, knockout, and overexpression transgenic plants were investigated. Compared with wild-type, knockout transgenic plants pCas9-1 and pCas9-2 decreased the grain length, increased grain width, increased grain thickness, and maintained 1000-grain weight. constant( image 3 , A and C-F). Compared with the wild-type phenotype, the overexpressed transgenic plants OE-2 and OE-19 had significantly lower 1000-grain weight, but there was no significant difference in grain length and grain width. Therefore, we investigated the grain thickness trait and the results showed that the overexpression plants Grain thickness was significantly reduced ( image 3 , A and C-F). At the same time, the investigation of grain type traits of brown rice found that compared with wild-type plants, knockout transgenic plants showed decreased grain length and increased grain width, while overexpression plants d...

Embodiment 3

[0074] Example 3 Detection of other agronomic characters

[0075] In the investigation of other agronomic traits, it was found that OsSMG6 also affected plant height and heading date ( Figure 4 , A and C-D). Compared with the wild type, the plant height of the knockout plants was increased and the heading date was delayed; while the plant height of the overexpression plants was decreased and the heading date was earlier. Comparing the internodes of the main stem and stem, the results showed that in the I internode and the III internode, the knockout plants were extremely significantly increased, while the overexpression plants were significantly reduced ( Figure 4 B). In addition, compared with the wild type, the transgenic plants showed no difference in the number of flag leaves and effective panicles; the width of flag leaves and panicle length of the overexpressed plants were significantly reduced ( Figure 4 , E-H). This phenomenon indicates that OsSMG6 has pleiotrop...

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Abstract

The invention provides a rice gene OsSMG6 and application thereof. The invention provides a rice gene OsSMG6 and application thereof. The invention clones a novel gene OsSMG6 (the gene has multiple effects due to one factor and also has influence on growth and development of plants while regulating and controlling the grain shape and the grain weight) for controlling the grain shape and the grain weight of the rice, and the function loss leads to shortening of the grain length, widening of the grain width, increase of the grain thickness, unchanged grain weight, increase of the plant height and delay of the heading period of the rice; the grain length and the grain width of the overexpressed transgenic plant are not changed, the grain thickness is reduced, and the grain weight is reduced; however, the brown rice is shortened in grain length, narrowed in grain width, shortened in ear length, narrowed in flag leaf, reduced in plant height and advanced in heading period. The OsSMG6 gene has important significance on research on a molecular mechanism of rice grain shape formation and influence on rice development, a high-quality gene resource is provided for rice variety improvement and breeding, and the rice breeding process is greatly promoted.

Description

technical field [0001] The present invention relates to the field of genetic engineering and plant genetics and breeding, in particular to the rice gene OsSMG6 and its application. Background technique [0002] Rice (Oryza sativa L.) is one of the most important commercial food crops, providing a staple food source for more than half of the world's population. my country has a long history of rice cultivation, the current total rice output ranks first in the world, and more than 65% of the population eats rice as the staple food. These phenomena all show that my country's rice production is closely related to world food security (Li Maomao et al., 2008; Xu Kuangdi and Shen Guofang). , 2002). Since the 1950s, through the application and innovation of high-quality germplasm resources, the yield level of rice has made two qualitative leaps. The cloning of the semi-dwarf gene sd1 and its application in rice breeding have successively bred semi-dwarf high-yield varieties suitabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46A01H1/02
CPCC07K14/415C12N15/8261C12N15/8218A01H1/021Y02A40/146
Inventor 张迎信程式华薛炮吴玮勋曹立勇陈代波刘群恩
Owner CHINA NAT RICE RES INST