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Chiral nano vaccine as well as preparation method and application thereof

A nano-vaccine and chiral technology, which is applied in the field of chiral nano-vaccine and its preparation, can solve the problems of poor cellular immune response, low safety, and weak effective presentation of antigens, and achieve strong humoral immune effect and strong ability to adsorb antigens , The effect of promoting capture efficiency

Pending Publication Date: 2022-08-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the technical problem to be solved by the present invention is to overcome the problems of weak antigen presentation, poor cellular immune response and low safety in the prior art.

Method used

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  • Chiral nano vaccine as well as preparation method and application thereof
  • Chiral nano vaccine as well as preparation method and application thereof
  • Chiral nano vaccine as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A hand -nano vaccine and its preparation methods include the following steps:

[0051] (1) Preparation of hand -hydrated nanoparticles: Soak the reaction bottle with king water for 24h, wash, and dry. Add 4ml of water to the reaction bottle, add 8mg / ml of potassium permanganate solution 1ml, 0.1ml oleic acid, 4.4 mg / ml of citrinine solution 0.3ml, 4mg / ml sodium hydroxide solution, 0.08ml of hydraulic acid, 0.08ml, and continuously stirring The color of the solution becomes black and stops stirring. Collect the reaction product 7000rpm centrifugal 5min, remove the upper clearing, sedimentation with pure water, repeatedly washing, and removing excess non -response.

[0052] Example 1 The transmitted electron microscopy and scanning electron microscopic diagram of the hand -pre -manganese nanoparticles prepared by the Example 1 figure 1 Show, figure 1 A, 1B, 1C, which are transmitted electron microscopy for L, D, DL, manganese nanoparticles; figure 1 D, 1E, 1F, are scanned ele...

Embodiment 2

[0058] A hand -nano vaccine and its preparation methods include the following steps:

[0059] (1) Preparation of hand -hydrated nanoparticles: Soak the reaction bottle with king water for 24h, wash, and dry. Add 4ml of water to the reaction bottle, add 8mg / ml of potassium permanganate solution 1ml, 0.1ml oleic acid, 4.4 mg / ml of citrinine solution (L type or D) 0.3ml, 4mg / ml hydrogen oxidation The sodium solution is 0.08ml, continuously stirred until the color of the solution becomes black, and stops stirring. Collect the reaction product 7000rpm centrifugal 5min, remove the upper clearing, sedimentation with pure water, repeatedly washing, and removing excess non -response.

[0060] (2) Preparation of hand-manganese oxide nano-vaccine: Frozen the synthetic hand-manganese nanoparticles at -30 ° C and 8Pa. ), Stir 12h, 7000rpm centrifugal 5min, remove the upper clearing, you can get a hand -to -hand manganese nano -vaccine.

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Abstract

The invention relates to a chiral nano vaccine as well as a preparation method and application thereof, and relates to the technical field of nano vaccines. The chiral nano vaccine disclosed by the invention comprises chiral metal oxide nano particles and an antigen, the chiral metal oxide nanoparticles are metal oxide nanoparticles with L chiral configuration and / or metal oxide nanoparticles with D chiral configuration. The chiral nano vaccine can activate inflammasome protein (NLRP3) and interferon gene stimulating protein (STING) at the same time, not only has a strong cellular immune effect and can promote an organism to resist tumor invasion and virus infection, but also has a strong humoral immune effect and can promote the organism to generate high-strength antibody titer, and the antibody titer can be maintained for 120 days.

Description

Technical field [0001] The invention involves the field of nano -vaccine technology, especially a hand -nano vaccine and its preparation methods and applications. Background technique [0002] Immune agents and antigen can enhance the antigenicity of the antigen and enhance the body's immune response. At present, the most commonly used agent in animal experiments is the Fahrenhaya, but the Fena fabric agent is an oil -soluble agent. It requires a complex emulsification process when mixing with water -soluble antigens, and is often accompanied by toxic side effects. Most of the adult agents used clinically are aluminum agents, but aluminum agents can only enhance the body's body fluid immunity and have poor stability. Therefore, new materials need to be used to develop safe and efficient immune agents. [0003] Due to its superior physical and chemical characteristics, nanomaterials are widely used in the field of biology. As a facilities, the surface characteristics of nanomateri...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/385A61K39/00A61P31/12A61P35/00
CPCA61K39/39A61K39/385A61K39/0005A61P35/00A61P31/12A61K2039/55505A61K2039/57A61K2039/545A61K2039/575A61K2039/62A61K2039/60Y02A50/30
Inventor 胥传来王伟炜匡华徐丽广孙茂忠吴晓玲刘丽强马伟朱建平郝昌龙宋珊珊胡拥明吴爱红郭玲玲胥欣欣
Owner JIANGNAN UNIV
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