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Production method of DHA (docosahexaenoic acid) algal oil rich in medium-chain fatty acid

A medium-chain fatty acid and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as ketone body poisoning, achieve the effect of increasing content, simple cultivation method, and low cost

Pending Publication Date: 2022-08-05
青岛即食未来生命科技有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, medium-chain fatty acids are not essential fatty acids, and excessive intake of them will lead to excessive ketone body poisoning, and cannot replace long-chain fatty acids to provide fatty acid nutrition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Taking Cryprhecodinium cohnii as the fermentation strain, in the fermentation process, 5% of the carbon source is replaced with medium-chain triglycerides, and the rest are cultivated according to the normal fermentation process, and after the cultivation is completed, it is concentrated by a centrifuge, The microalgae is broken by enzymatic wall-breaking, and the fat is extracted, and then through degumming, deacidification, decolorization, deodorization and other refining processes, DHA algal oil is finally obtained. The sum of , capric acid and lauric acid was 2.8%, and the DHA content of medium and long chain glycerides was 3.2%.

Embodiment 2

[0021] Taking Cryprhecodinium cohnii as the fermentation strain, coconut oil was used to replace 15% of the carbon source in the fermentation process, and the rest were cultivated according to the normal fermentation process. The wall breaks the microalgae, extracts the fat, and then undergoes degumming, deacidification, decolorization, deodorization and other refining processes to finally obtain DHA algae oil, and gas phase detection is used in the finished DHA algae oil. The sum of lauric acid is 3.5%, and the DHA content of medium and long chain glycerides is 4.5%.

Embodiment 3

[0023] Taking Schizochytrium sp. as the fermentation strain, in the fermentation process, 8% of the carbon source was replaced by caprylic acid glyceride, and the rest were cultivated according to the normal fermentation process. The wall-breaking method breaks the microalgae, extracts the fat, and then undergoes degumming, deacidification, decolorization, deodorization and other refining processes, and finally obtains DHA algal oil. The finished DHA algal oil is detected by gas phase detection. The sum of acid and lauric acid was 5.4%, and the DHA content of medium and long chain glycerides was 6.2%.

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Abstract

The invention provides a production method of DHA (docosahexaenoic acid) algal oil rich in medium-chain fatty acid, which comprises the following steps: an inducer is added in a microalgae culture process, and the inducer comprises at least one of hexanoic acid, octanoic acid, decanoic acid, coconut oil, palm kernel oil, soybean oil and medium-chain triglyceride. According to the production method of the DHA algal oil rich in medium-chain fatty acid, the specific attractant is added in the microalgae culture process, the content of the medium-chain fatty acid in the algal oil obtained through fermentation is increased, the culture mode is simple, the cost is low, the economic value of the algal oil is increased, and good market prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a method for producing DHA algal oil rich in medium-chain fatty acids. Background technique [0002] Docosahexaenoic acid (DHA) is a very important polyunsaturated fatty acid for the human body, commonly known as brain gold. According to the "Food Safety Law of the People's Republic of China" and the "Administrative Measures for New Resource Foods", approved sources from Schizochytrium sp. or Ulkenia amoeboida or Cryprhecodinium cohnii ) species are bacterial species raw materials, and the algal oil DHA produced by processes such as fermentation, separation, and purification is used as a new resource food. There are two main sources of DHA. One is from deep-sea fish oil and the other is from microalgae. Compared with DHA from deep-sea fish oil, DHA from algae oil has the advantages of plant origin, low content of harmful substances, low content of EPA and less ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/6409C12P7/6463C12R1/89
CPCC12P7/6409C12P7/6463C12R2001/89
Inventor 白长军邵振文王永富李浩赵汉杰罗丹
Owner 青岛即食未来生命科技有限公司
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