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ELISA (enzyme-linked immunosorbent assay) kit for quantitatively detecting bovine immunoglobulin G and detection method thereof

A technology for quantitative detection of immunoglobulin, which is applied to the ELISA kit for quantitative detection of bovine immunoglobulin G and its detection field, can solve the problems of detection of residual bovine IgG content, etc., and achieve low cost, high sensitivity, and low detection range Effect

Pending Publication Date: 2022-08-05
上海赛唐生物技术有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The related products of this patent can only be used to detect the content of bovine colostrum in health food, but cannot be used to detect the content of residual bovine IgG in biomedical products
[0005] For the detection of bovine IgG residues in biopharmaceutical products, there is no legal regulation for this detection in the "Chinese Pharmacopoeia" and relevant departments

Method used

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  • ELISA (enzyme-linked immunosorbent assay) kit for quantitatively detecting bovine immunoglobulin G and detection method thereof
  • ELISA (enzyme-linked immunosorbent assay) kit for quantitatively detecting bovine immunoglobulin G and detection method thereof
  • ELISA (enzyme-linked immunosorbent assay) kit for quantitatively detecting bovine immunoglobulin G and detection method thereof

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Embodiment Construction

[0029] In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further described below.

[0030] 1. Experimental materials

[0031] stabilizer;

[0032] Bovine serum albumin powder, no protease / DNAse;

[0033] Trehalose, Na 2 CO 3 , NaHCO 3 , KH 2 PO 4 , K 2 HPO 4 , Na 2 HPO 4 ·12H 2 O, Tween 20, NaCl, KCl;

[0034] Washing solution, chromogenic solution A, chromogenic solution B, stop solution;

[0035] Coating antibody: rabbit anti-bovine IgG, antigen: bovine IgG Detection antibody: rabbit anti-bovine IgG;

[0036] Recycling experiment buffer1 formula: 1.25mLbuffer1 formula:

[0037]

[0038] Dissolve the above raw materials into 1.25 mL of 0.15 M PH 7.4 PBS.

[0039] Recovery experiment buffer2: CHO serum-free protein-free chemical medium;

[0040] ELISA coating buffer----(pH9.6 0.05M carbonate buffer)

[0041] Na 2 CO 3 1.59g;

[0042] NaHCO 3 2....

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Abstract

The invention provides an ELISA (enzyme-linked immuno sorbent assay) kit for quantitatively detecting bovine immune globulin G and a detection method thereof. The kit comprises an ELISA coating buffer solution, a rabbit anti-bovine immunoglobulin G antibody storage solution, an ELISA microwell plate coated with a rabbit anti-bovine immunoglobulin G antibody, a bovine immunoglobulin G storage solution, an antibody storage solution marked by rabbit anti-bovine immunoglobulin G chromogenic substrates, an ELISA concentrated washing solution, a special ELISA diluent, an ELISA chromogenic substrate A solution, an ELISA chromogenic substrate B solution and a stop solution. Through the kit, the invention further provides the ELISA kit for quantitatively detecting the bovine immunoglobulin G, which is high in detection precision and simple and operable in detection mode, and a detection method of the ELISA kit.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to an ELISA kit for quantitative detection of bovine immunoglobulin G and a detection method thereof. Background technique [0002] Bovine immunoglobulin G (Bovine immunoglobmlin G) is the main component of immunoglobulin in bovine serum, with a molecular weight of about 160kDa, of which 40-50% is distributed in serum, and the rest is distributed in tissues. IgG includes four subtypes, IgG1, IgG2, IgG3, and IgG4, and the content in serum is 17-27 mg / ml. [0003] Bovine IgG (Bovine IgG) is widely used in basic biochemistry, teaching, scientific research, biomedical production and other fields. In the production of recombinant protein drugs, bovine serum albumin is often added to mammalian cell culture media. Even if it is purified and removed by a certain process, a small amount of bovine IgG may still remain in recombinant protein drugs and other final products. As a sensitizin...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/543
CPCG01N33/6854G01N33/543G01N33/581
Inventor 李浪程梦楠叶小飞
Owner 上海赛唐生物技术有限公司
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