Aspirin hapten, aspirin complete antigen, aspirin antibody, preparation method and application
An aspirin, complete antigen technology, applied in the field of antibody and preparation, aspirin hapten, complete antigen, can solve the problem of lack of high-throughput, simple and rapid detection methods, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0035] The present invention provides the preparation method of the aspirin hapten derivative described in the above technical scheme, comprising the following steps:
[0036] The salicylic acid, 5-bromovaleryl chloride and Lewis acid catalyst are mixed (hereinafter referred to as the first mixture) to undergo an acylation reaction to obtain the aspirin hapten derivative of the structure described in formula 1.
[0037] In the present invention, unless otherwise specified, the raw materials used are all commercially available products well known to those skilled in the art.
[0038] In the present invention, the Lewis acid catalyst is preferably anhydrous aluminum trichloride.
[0039] In the present invention, the mass ratio of the salicylic acid and 5-bromovaleryl chloride is preferably 6.9:20.
[0040] In the present invention, the mass ratio of the salicylic acid and the Lewis acid catalyst is preferably 6.9:0.5.
[0041] In the present invention, the first mixing sequen...
Embodiment 1
[0135] according to figure 1 In the reaction equation shown, 6.9 g of salicylic acid (compound 1), 20.0 g of 5-bromovaleryl chloride (compound 2), and 0.5 g of anhydrous aluminum trichloride were added to a 50 mL three-necked flask. The temperature was raised to 60-65 °C, and the reaction was kept stirring for 1 h. TLC monitored the completion of the reaction and cooled the temperature to 30-35 °C, slowly added 30 mL of deionized water, controlled the temperature to 0-10 °C for crystallization for 1 h, and filtered to obtain 11.24 g of compound 3 crude product.
[0136] Add 11.0 g of compound 3 to 40 mL of ethanol in a 50 mL three-necked flask, heat up to 50 °C for dissolving for 30 min, filter, cool the filtrate to 0-5 °C for 2 h, filter, and vacuum dry to obtain 10.05 g of compound 3 in a yield of 67.1%. The HNMR spectrum of the aspirin hapten derivatives prepared in this example is as follows: image 3 shown.
Embodiment 2
[0138] Take 10 mg of the aspirin hapten derivative prepared in Example 1, add 1 mL of DMSO to dissolve completely, and obtain an aspirin hapten derivative solution;
[0139] Dissolve 20 mg of bovine thyroglobulin in 5 mL, 0.1 mol / L sodium bicarbonate solution to obtain a bovine thyroglobulin solution;
[0140] Mix the aspirin hapten derivative solution and bovine thyroglobulin solution, stir at room temperature for 2 hours to obtain the coupling reaction solution, dialyze the coupling reaction solution with PBS buffer solution for 4 times to obtain the aspirin complete antigen, which is frozen at -20 ℃ save.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More