Preparation method and structural characterization method of red ginseng HG type pectin
A technology of red ginseng and pectin, which is applied in the fields of instruments, nuclear magnetic resonance analysis, and material analysis, etc. It can solve the problems of difficult to accurately characterize the structure, the preparation method of red ginseng HG pectin has not been reported, and the complex composition of monosaccharides.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0060] The present invention obtains a preparation method of red ginseng HG-type pectin through a large number of experimental studies, and can prepare high-purity red ginseng HG-type pectin. The preparation method comprises the following steps:
[0061] S1. Extract the total polysaccharide of red ginseng with a molecular weight of ≥3500Da by water extraction and alcohol precipitation;
[0062] S2, the total polysaccharide is enzymolyzed with amylase to obtain an enzymolysis solution;
[0063] S3. Use weak anion exchange cellulose as the stationary phase, and sequentially use water and 0.05M, 0.1M, 0.2M, 0.3M, 0.4M, and 0.5M NaCl aqueous solutions as elution solvents, and perform a column layer on the enzymatic hydrolysis solution. For analysis, the eluates obtained with different elution solvents were collected respectively;
[0064] S4. Adjust the pH value of the eluent to be neutral with hydrochloric acid, and after concentration, dialysis to obtain a sample with a molecu...
Embodiment 1
[0074] This embodiment provides a preparation method of red ginseng HG type pectin.
[0075] 1. Preparation of non-starch polysaccharides
[0076]The red ginseng medicinal materials were crushed into coarse particles, and then further crushed with a pulverizer, passed through a 60-mesh sieve, and 4 kg of powder was added to 10 times the amount of water and placed in an ultrasonic extractor in a 70°C water bath. Ultrasonic at 40kHz (400W) After extraction for 1 hour, the extract was cooled to room temperature, and then centrifuged at 4000rpm for 20min using a centrifuge. The supernatant was collected and added with 4 times the volume (v / v) of absolute ethanol (slowly accelerated stirring) until the alcohol content in the sample was 80%. (v / v), the samples after alcohol precipitation were left to stand overnight. After about 24 hours of alcohol precipitation, the samples were centrifuged at 4000 rpm for 5 minutes, then the precipitates were collected and dried in an oven at 60 ...
Embodiment 2
[0084] This embodiment provides a structure characterization method for red ginseng HG-type pectin. The sample prepared by using the 0.2M NaCl aqueous solution as the elution solvent in Example 1 and the eluent obtained by elution with the resin bed volume for the third time (that is, figure 1 Samples marked in dashed boxes. Hereinafter, the code name RG0203 is used as the test product for the structural characterization of this embodiment.
[0085] 1. Structural characterization
[0086] 1.1 Molecular weight calibration based on HPSEC-MALLS-RID
[0087] Preparation of sample solution: Weigh about 5 mg of RG0203 obtained in Example 1, add water to prepare 5 mg / mL, centrifuge at 14,000 rpm for 10 min and filter it with a 0.22 μm filter membrane to serve as the test solution;
[0088] The LC-20AD HPSEC system, equipped with RID-20A detector and DAWN 8HELEOS MALLS detector, was used for data collection and analysis of red ginseng HG pectin in the test solution. Chromatographic...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More