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Swinging unit

A technology of swinging device and elastic sheet, which is applied in the directions of axle suspension, transportation and packaging, mechanical equipment, etc. It can solve the problems of unreliable results, unreliable detection results, time-consuming and other problems

Inactive Publication Date: 2004-09-15
DAHON INTELLIGENCE PROPERTY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(References: "Practical Clinical Bacteriology Examination and Progress" edited by Lou Yongxin and Wang Jinliang, 1992 Tianjin Science and Technology Translation Publishing Company), the results of this method are relatively accurate but very time-consuming, and the detection results of new species are not very reliable; 2, Rapid bacterial identification methods such as API-20E, which use statistical analysis to draw identification conclusions after detecting a few biochemical indicators, relatively save time, but the results are not reliable

Method used

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Experimental program
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Effect test

Embodiment 1

[0029] Make a kit for rapid detection of algae Schwannella according to the following formula:

[0030] Reagent A: 0.1M NaCl, 10mM Tris.Cl (pH8.0), 1mM EDTA (pH8.0), 5% Triton X-100

[0031] Reagent B: 10mg / ml lysozyme, 10mM Tris.Cl (pH8.0)

[0032] Reagent C: 5.0μl 10×PCR Buffer, 0.5μl Taq (5U / μl), 35.5μl ddH 2 o

[0033] Reagent D: 4.0μl dNTP (2.5mM each), 3.0μl MgCl 2 (25mM),

[0034] 20pm VP 03 , 20pm VP 04

[0035]Reagent E: 50×TAE

[0036] Reagent F: agarose (1%) + ethidium bromide (0.5 μg / ml)

[0037] Follow the procedure below for testing:

[0038] 1. Sample processing and DNA extraction: Take about 0.1-0.5 grams of fish tissue, add 400 μl of reagent A to the sample, mash it with a sterilized toothpick, centrifuge at 12,000 g for 2 minutes, discard the supernatant, and re-use 350 μl of reagent A and 50 μl of reagent B. Suspend the precipitate and mix well. Place at room temperature for 5 minutes, then in a water bath at 100°C for 5 minutes. Cool to r...

Embodiment 2

[0044] Various solutions were prepared according to the following recipes:

[0045] Reagent A: 0.3M NaCl, 10mM Tris.Cl (pH8.0), 1mM EDTA (pH8.0),

[0046] Reagent B: 5mg / ml lysozyme, 10mM Tris.Cl (pH8.0)

[0047] Reagent C: 2.5μl 10×PCR Buffer, 0.25μl Taq (5U / μl), 35.5μl ddH 2 o

[0048] Reagent D: 2.0μl dNTP (2.5mM each), 1.5μl MgCl 2 (25mM),

[0049] 10pm VP 03 (0.5μl), 10pm VP 04 (0.5μl)

[0050] Reagent E::50×TAE

[0051] Reagent F: agarose (1.2%) + ethidium bromide (0.8 μg / ml)

[0052] Follow the procedure below:

[0053] 1. Sample Processing and DNA Extraction

[0054] Take about 0.1-0.5 g of fish tissue, add 200 μl of reagent A to the sample, mash it with a sterilized toothpick, centrifuge at 12,000 g for 2 minutes, discard the supernatant, resuspend the pellet with 150 μl of reagent A and 50 μl of reagent B, and mix well. Place at room temperature for 5 minutes, then in a water bath at 100°C for 1 minute. Cool to room temperature and centrifuge at 12000g fo...

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Abstract

A swinging unit is composed of base, force-applied body and more than two elestic leaves connected between base and force-applied body by fixing connection at one end and hanging connection at another end. When a force is applied to it, it can swing back and forth. Its advantages are great swinging amplitude and high yield strength.

Description

technical field [0001] The invention relates to a kit for rapidly detecting algae Schwannella, and further relates to a method for rapidly detecting algae Schwannella using the kit for rapidly detecting algae Schwannella. Background technique [0002] In recent years, my country's mariculture industry has developed rapidly, and the output of mariculture has increased by 5-6 times in the past 10 years. However, the large-scale development of mariculture in the previous stage was very blind and even destructive, which led to the slowdown in the growth of mariculture production in the past two years, especially the problem of disease has become more and more serious. Among them, vibriosis is one of the important pathogens that harm a variety of mariculture organisms such as fish, shrimp, shellfish and algae. Vibrio is an indigenous bacterium of marine origin, widely distributed in seawater and marine organisms; pathogenic Vibrio can cause explosive death of cultured organisms,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B62J1/02B62K25/04B62K25/28F16F1/26F16F3/00
CPCB62K25/28B62K2201/06F16F3/00B62K25/04B62J1/02F16F1/26
Inventor 韩德玮
Owner DAHON INTELLIGENCE PROPERTY CO LTD