Li's Trichoderma strains and use thereof

A technology of Trichoderma reesei and bacterial strains, applied in the direction of fungi, methods of using microorganisms, enzymes, etc., can solve the problems of low activity and achieve high activity, fast growth, and convenient cultivation

Inactive Publication Date: 2005-01-19
HANGZHOU TIANKAI BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Usually, enzymes produced by Trichoderma and Fusarium have high C 1 Activity, Aspergillus niger cellulase is mainly C x Enzyme and beta-glucosidase, C 1 less active

Method used

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  • Li's Trichoderma strains and use thereof
  • Li's Trichoderma strains and use thereof

Examples

Experimental program
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Embodiment Construction

[0017] 1 Strain screening and mutagenesis

[0018] 41 strains that can grow on wheat bran were screened from soil humus, and the enzyme activities of β-glucanase and xylanase were measured respectively. There were 12 strains with these two enzyme activities at the same time. Planted on the Congo red lichen polysaccharide plate, 5 strains with larger transparent circles were obtained.

[0019] These 5 strains were cultured and made into a single spore suspension, which were subjected to ultraviolet (irradiation at 30 cm under a 30W ultraviolet lamp for 60s) and radiation (Co 60 Irradiation for 10 seconds) and chemical reagents (nitrosoguanidine final concentration 1mg / ml, 28 standstill for 60min) mutagenesis, after mutagenesis, the spores are spread on the primary screening plate, and the strains that form a clear transparent circle are picked. Then the enzyme activities of β-glucanase and xylanase were measured separately, and it was found that one strain had particularly high enz...

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Abstract

The present invention discloses a kind of Li's trichloderma strain and its application. Said strain is grown on the PDA solid culture medium, its formed colony is light-green byssoid, its edge is white and regular, its growth speed is rapid, its hypha is white, has septum, hyphal wall is smooth and its diameter is 2.0-5.0 micrometer. Its conidiophore is generated from short lateral branch of hypha, and opposite on the lateral branch. Said conidiophore is pitcher-shaped, vertical, colorless, its spore is green, and is ball-shaped, its diameter is 20-100 micrometers. Said invention possesses the following advantages: 1. convenient culture, its growth speed is rapid; 2. can simultaneously produce two feed enzyme preparations, beta-glucanase and xylanase with high activity.

Description

Technical field [0001] The invention relates to a Trichoderma reesei strain and its use. Background technique [0002] With the improvement of people's living standards, animal husbandry has also developed rapidly. Feed cost is the main input of animal husbandry production, accounting for 60-80% of the total cost. At present, the main energy raw material for feed on the market is corn. Among the many grains, the reason why the feed manufacturers choose corn is that the energy value and digestibility of corn are higher. In contrast, other grains such as barley, wheat, rice, etc. and their by-products contain higher anti-nutrients. Material, which reduces their feeding value. Of course, these grain feeds and their by-products also have their own advantages. First, the crude protein content is relatively high, which is usually 1-6% higher than that of corn. Second, the price is relatively low. Secondly, wheat, The secondary powder can increase the cohesive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/22C12N9/42C12N9/46
Inventor 许梓荣孙建义李卫芬
Owner HANGZHOU TIANKAI BIOLOGICAL TECH CO LTD
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