Method for detecting transgenic plant and products thereof

Abstract

The present invention relates to a transgenic plant and detection method of its product. Said invention uses the characteristic sequence being in transgenic plant: T-DNA left anjd right boundary sequence, CaMV 35S promotor, CaMV 35S poly A, nos promotor and nos termination sequence to design and synthesize initiator to make PCR to obtain correspondent DNA amplification product or signal of amplification product. According to the PCR result it can be used for recognizing that said detected sample is transgenic plant and its product or not.

Description

technical field [0001] The invention belongs to the technical field of transgenic plant safety management and the field of biotechnology, and in particular relates to a rapid detection method for transgenic plants and products thereof. Background technique [0002] 1. Safety management of transgenic plants [0003] A transgenic plant refers to a plant variant that artificially transfers an exogenous gene into the plant genome through a certain method, and then inherits and expresses it. The exogenous gene transferred into the plant genome is called a transgene. [0004] In 1983, humans first reported the birth of genetically modified tobacco. By 2000, the global planting area of ​​genetically modified plants had reached 44.2 million hectares. Since the birth of genetically modified plants, some people have worried about their possible ecological and food safety problems, and these concerns have been supported by some research results. In...

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Problems solved by technology

But in the past, people mainly aimed at the target gene, reporter or marker gene. The limitation is that if a specific plant does not know which gene the researcher introduces, and if a product contains multiple plant components, the above method will have great risks. Blindness, it takes a long time to determine whether it (they) are genetically modified plants or products

[0008] (2) RNA molecular hybridization (Northern hybridization), reverse transcription PCR (RT-PCR) and other analysis techniques are used to detect the expression status of the target gene at the RNA level. The defects are the same as above, and the test samples must be fresh. Plant materials, unable to detect dry, dead materials and various products made from plants

[0009] (3) Protein ELISA, Western blot and other analysis techniques use the immune reaction between antibodies and antigens to analyze the expression status of genes at the protein level. The disadvantage is that if a specific plant does not know which gene the researcher introduces, If a product contains multiple plant components, it will be difficult to determine which gene product antibody to use for detection

Although there are several reports using the CaMV 35S promoter to detect transgenic plants and their products, for CaMV-susceptible cruciferous plants, the detection of the CaMV 35S promoter alone may cause important errors