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Method for administering viable microorganism composition for poultry

A technology of active microorganisms and compositions, applied in the directions of drug combination, application, animal feed, etc., can solve the problems of long-term continuous use, poor settlement ability, and hindering practical application.

Inactive Publication Date: 2000-09-06
ASAHI CALPIS WELLNESS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some areas where chick rearing is often practiced, commercial drug-free rearing of chicks in this manner is extremely difficult due to the frequent infection of chicks with toxic bacteria that can easily spread through feedlots as described above
Also, even if drug-free chicks could be produced this way, the rate of weight gain in poultry would be slower than if reared with drugs
As a result, high yields using medicated feeding cannot be obtained
[0010] In addition, active microbial preparations currently on the market have problems such as a reduction in the number of active bacteria in the dispensing step, poor colonization ability after the formation of intestinal flora, and the need to continuously take the active microbial preparations for a long time, etc.
In particular, from an economic point of view, since the colonization ability of the intestinal flora is poor after formation, it is necessary to continuously take active microbial preparations for a long time, which seriously hinders its practical application at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A 4.5 gram portion of skim milk was dissolved in 50 grams of water, pasteurized at 100°C for 10 minutes, then cooled to room temperature. Inoculate an inoculation loop of Lactobacillus reuteri CP-720 into the solution thus prepared, and culture it statically at 37°C for 24 hours to obtain primary primers (lactic acid bacteria: 1×10 8 active microorganisms / g). Next, 15 grams of primary primers were inoculated into 500 grams of pasteurized skim milk (90% solid content by weight) at 90°C, and cultivated at 37°C for 20 hours to obtain secondary primers. The secondary primer contains 2×10 8 Viable microorganisms / g.

[0047] A medium was prepared by dissolving 200 g of casein peptone, 200 g of yeast extract, 100 g of sodium citrate and 200 g of glucose in 20 kg of water, and the pH of the medium was adjusted with 1N NaOH solution, and the medium was placed in a fermenter pasteurize at 95°C for 15 minutes, then cool to room temperature. Then 3 parts by weight of the above-...

Embodiment 2

[0050] Dissolve 200 grams of beef peptone, 60 grams of soybean peptone, 100 grams of yeast extract, 100 grams of sodium acetate, 40 grams of dipotassium hydrogen phosphate, 60 grams of diammonium citrate and 400 grams of glucose in 20 kg of water to prepare a medium, and use 1N NaOH solution adjusted the pH of the medium, which was placed in a fermenter, pasteurized at 95°C for 15 minutes, and then cooled to room temperature. Then, the secondary primer of 500 g of Lactobacillus johnsonii CP-721 pre-cultivated in the same way as in Production Example 1 was inoculated into the culture medium, and static culture was carried out at 35° C. for 18 hours.

[0051] The obtained culture solution is centrifuged so as to reclaim the microorganisms, and then freeze-dried using a dispersion medium. The culture medium solution contains 10% by weight of skim milk and 1% by weight of sodium glutamate. Pasteurization was carried out at 90° C., so 141 grams of Lactobacillus johnsonii CP-721 act...

Embodiment 3

[0053] The secondary primer of Lactobacillus reuteri CP-722 prepared by the same method as Production Example 1 was inoculated into the medium prepared and pasteurized by the same method disclosed in Production Example 1, which had The same ingredients composition and weight, and static culture at 37°C for 20 hours. The culture solution thus obtained was centrifuged to collect the cells, and then the cells were freeze-dried using a dispersion medium, which was a solution containing 10 wt% skim milk and 1 wt% sodium glutamate. The solution was previously pasteurized at 95° C., thus obtaining 151 grams of active microbial dry powder of Lactobacillus reuteri CP-722. The active microbial dry powder contains 6.8×10 10 Viable microorganisms / g.

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PUM

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Abstract

A method for administering viable microorganism compositions for poultry, comprising: administering a viable microorganism composition comprising viable lactic acid bacteria belonging to Lactobacillus reuteri and Lactobacillus johnsonii at the stage of newborn fledgling; and administering a viable microorganism composition which comprises a viable microorganism belonging to Bacillus subtilis.

Description

technical field [0001] The present invention relates to a method for administering an active microbial composition to poultry, wherein an active microbial composition useful for poultry growth is administered in order to raise poultry with high productivity while reducing the amount of medicines such as antibiotics, antibacterial agents, etc. , or without the use of drugs. Background technique [0002] In poultry rearing such as chicks, in order to reduce production costs, it is common to carry out 2 ) Intensive breeding of 40-60 birds in poultry farms. In addition, the birds are fattened to achieve rapid growth, gaining an average of 50-55 grams of body weight per day, so that the birds can be marketed approximately 2 months after hatching. Poultry are often stressed under these conditions, they often get sick, and disease often spreads among the birds. In ground feeding, the poisonous bacteria can easily spread to all the poultry in the farm as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B66C7/12A61K35/74A61K35/747A61P3/00A61P31/00B66C19/00C12N1/20
CPCC12N1/20A23K1/009A61K35/742A61K35/747A23K1/1826A23K1/182A23K10/18A23K50/70A23K50/75A61P3/00A61P31/00A61P43/00A61K2300/00
Inventor 丸田喜义宫崎博
Owner ASAHI CALPIS WELLNESS CO LTD
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