Two-way rotary hot wind exchanging type real time fluorescent quantitative poly enzyme chain reaction analyzer

Abstract

The invention discloses a real-time fluorescence quantitative polymerase chain reaction analyzer of a bidirectional rotating hot air exchange type, which comprises a box body, a motor and a main shaft are arranged in the box body, and a hot air exchange circulation device is arranged at the upper end of the box body, and the hot air exchange circulation device Including the upper wind room arranged on the upper part of the box body, the upper wind room is equipped with an air inlet pipe with a hole at the top, a powerful fan and a heater group are arranged in the air inlet pipe, the powerful fan is located at the upper end of the heater group, and the heater group is equipped with Overheating protection device, the air outlet of the air inlet pipe is equipped with an amplification chamber, the amplification chamber is located in the box and communicated with the upper air chamber through a porous ring-shaped air return port, the amplification chamber is equipped with a temperature sensor, and the upper air chamber is equipped with a controllable fan unit and safety guards. The invention has the characteristics of automatic monitoring, rapidity, accuracy, non-pollution, convenient operation, low price, easy popularization, etc., and is suitable for the needs of grassroots medical and health, epidemic prevention, animal and plant detection and other units.

Description

technical field [0001] The invention is applicable to an automatic and rapid quantitative analysis device for polymerase chain reaction (PCR) original target gene concentration in the fields of medicine and biology, and more specifically relates to a real-time fluorescence quantitative polymerase chain reaction analyzer of bidirectional rotating hot air exchange type. Background technique [0002] PCR is the abbreviation of polymerase chain reaction (Polymerase chain reaction), which is a molecular biology technique that amplifies a large amount of specific DNA fragments in vitro in a short period of time. Ordinary PCR instruments first place the target gene in a thermal cycle device controlled by a microcomputer automatic control circuit for heating and cooling, and complete the denaturation, renaturation and extension required by PCR, so that the target gene is amplified millions of times and completes PCR amplification. Afterwards, the separation an...

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Problems solved by technology

The mode and device of this amplification have the same deficiency as the previous patent

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